NucleoSpin Plasmid Transfection-grade

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Nucleic acid purification

Tech Note

Endotoxins are copurified during plasmid preparations from E. coli lysates and interfere with eukaryotic cell viability, making endotoxin reduction an essential step prior to cell transfection. NucleoSpin Plasmid Transfection-grade incorporates an innovative technology to reduce endotoxins from bacterial lysates compared to standard plasmid miniprep kits. The NucleoSpin Plasmid Transfection-grade and NucleoSpin 96 Plasmid Transfection-grade kits are designed for rapid, manual or automated, small-scale purification of transfection-grade plasmid DNA from E. coli.

If your cells are especially sensitive to endotoxin, see also Nucleobond Xtra EF products for the lowest possible endotoxin levels in your plasmid preps.

At-a-glance Cell viability Choose the right kit

At-a-glance

Technology	Silica-membrane and endotoxin-reduction technology
Format	Mini spin columns/96-well plates
Endotoxin content	<50 EU/μg DNA (EU = Endotoxin Units)
Sample material	Up to 5-ml bacterial culture
Vector size	<15 kbp
Typical yield	5–20 μg
Elution volume	30–200 μl
A₂₆₀/A₂₈₀	1.80–1.85
Preparation time	14 min/6 preps or 45 min/96 preps
Binding capacity	35 μg

Cell viability

Choose the right kit

Assessing endotoxin content of plasmids purified with various Macharey-Nagel products.

Order Information

Cat. #	Product	Size	Price
740490.50	NucleoSpin® Plasmid Transfection-grade	50 Preps	USD $105.00
NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Different plasmid purification technologies yield endotoxin levels appropriate for individual applications Different plasmid purification technologies yield endotoxin levels appropriate for individual applications. A quantitative chromogenic Limulus Amebocyte Lysate (LAL) test was used to assess endotoxin content. As indicated, endotoxin content depends strongly on the plasmid purification technology used. The NucleoSpin Plasmid Transfection-grade kit yields a plasmid solution containing sufficiently low endotoxin levels for direct transfection into common cell lines. . Back Compatibility of eluted DNA with transfection host cells Compatibility of eluted DNA with transfection host cells. A pCMV-GFP plasmid (kindly provided by PlasmidFactory GmbH und Co. KG, Bielefeld, Germany) was purified from E. coli using the NucleoSpin Plasmid Transfection-grade kit. The purified plasmids were transfected into HEK239 cells by lipofection (Lipofectamine 2000) or nucleofection (Lonza), with a >90% transfection ratio in both cases. Survival rates of cells transfected with the eluted DNA were compared to controls containing no added DNA. The similar ratios observed indicated that cell survival is not affected by addition of DNA eluates purified with the NucleoSpin Plasmid Transfection-grade kit. . Back The NucleoSpin Plasmid Transfection-grade procedure The NucleoSpin Plasmid Transfection-grade procedure. Back 740490.50: NucleoSpin Plasmid Transfection-grade
740490.250	NucleoSpin® Plasmid Transfection-grade	250 Preps	USD $440.00
NucleoSpin Plasmid Transfection-grade is a fast miniprep kit that uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740490.250: NucleoSpin Plasmid Transfection-grade Back Different plasmid purification technologies yield endotoxin levels appropriate for individual applications Different plasmid purification technologies yield endotoxin levels appropriate for individual applications. A quantitative chromogenic Limulus Amebocyte Lysate (LAL) test was used to assess endotoxin content. As indicated, endotoxin content depends strongly on the plasmid purification technology used. The NucleoSpin Plasmid Transfection-grade kit yields a plasmid solution containing sufficiently low endotoxin levels for direct transfection into common cell lines. . Back Compatibility of eluted DNA with transfection host cells Compatibility of eluted DNA with transfection host cells. A pCMV-GFP plasmid (kindly provided by PlasmidFactory GmbH und Co. KG, Bielefeld, Germany) was purified from E. coli using the NucleoSpin Plasmid Transfection-grade kit. The purified plasmids were transfected into HEK239 cells by lipofection (Lipofectamine 2000) or nucleofection (Lonza), with a >90% transfection ratio in both cases. Survival rates of cells transfected with the eluted DNA were compared to controls containing no added DNA. The similar ratios observed indicated that cell survival is not affected by addition of DNA eluates purified with the NucleoSpin Plasmid Transfection-grade kit. . Back The NucleoSpin Plasmid Transfection-grade procedure The NucleoSpin Plasmid Transfection-grade procedure.
740491.1	NucleoSpin® 96 Plasmid Transfection-grade	96 Preps	USD $364.00
The NucleoSpin 96 Plasmid Transfection-grade kit uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates, in a 96-well-plate format. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740491.1: NucleoSpin 96 Plasmid Transfection-grade Back NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor. Plasmid DNA was isolated from 3 ml of an E. coli culture (A600=2) with the NucleoSpin 96 Plasmid Transfection-grade kit and a 96-well format kit from Competitor Z. DNA yield was measured by absorption readings. Back NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor. Plasmid DNA was isolated from 3 ml of an E. coli culture (A600=2) with the NucleoSpin 96 Plasmid Transfection-grade kit and a 96-well format kit from Competitor Z. DNA purity was assessed by absorption readings. An A_260/A280 ratio of 1.8 was obtained for plasmid DNA isolated with the NucleoSpin 96 Plasmid Transfection-grade kit, indicating the absence of contaminating compounds (e.g., RNA or proteins). Back The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields. High-copy plasmid DNA was isolated from 3 ml of an E. coli culture with the NucleoSpin 96 Plasmid Transfection-grade kit, and the eluates were loaded on a 1% TAE agarose gel and stained with ethidium bromide. Lane M: 1 kb ladder (Thermo Scientific). Lanes 1–23: Eluted plasmid samples. Back The NucleoSpin Plasmid 96 Transfection-grade procedure The NucleoSpin Plasmid 96 Transfection-grade procedure.
740491.24	NucleoSpin® 96 Plasmid Transfection-grade	24 x 96 Preps	USD $6385.00
NucleoSpin 96 Plasmid Transfection-grade kit (24 x 96 prep version) uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates, in a 96-well-plate format. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740491.24: NucleoSpin 96 Plasmid Transfection-grade Back NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor. Plasmid DNA was isolated from 3 ml of an E. coli culture (A600=2) with the NucleoSpin 96 Plasmid Transfection-grade kit and a 96-well format kit from Competitor Z. DNA yield was measured by absorption readings. Back NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor. Plasmid DNA was isolated from 3 ml of an E. coli culture (A600=2) with the NucleoSpin 96 Plasmid Transfection-grade kit and a 96-well format kit from Competitor Z. DNA purity was assessed by absorption readings. An A_260/A280 ratio of 1.8 was obtained for plasmid DNA isolated with the NucleoSpin 96 Plasmid Transfection-grade kit, indicating the absence of contaminating compounds (e.g., RNA or proteins). Back The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields. High-copy plasmid DNA was isolated from 3 ml of an E. coli culture with the NucleoSpin 96 Plasmid Transfection-grade kit, and the eluates were loaded on a 1% TAE agarose gel and stained with ethidium bromide. Lane M: 1 kb ladder (Thermo Scientific). Lanes 1–23: Eluted plasmid samples. Back The NucleoSpin Plasmid 96 Transfection-grade procedure The NucleoSpin Plasmid 96 Transfection-grade procedure.
740492.4	NucleoSpin® 96 Plasmid Transfection-grade Core Kit	4 x 96 Preps	USD $1135.00
The NucleoSpin 96 Plasmid Transfection-grade kit uses a new technology developed by Macherey-Nagel to reduce the level of endotoxins co-purified during plasmid preparations from bacterial lysates, in a 96-well-plate format. Since endotoxins interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor. Plasmid DNA was isolated from 3 ml of an E. coli culture (A600=2) with the NucleoSpin 96 Plasmid Transfection-grade kit and a 96-well format kit from Competitor Z. DNA yield was measured by absorption readings. Back NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor. Plasmid DNA was isolated from 3 ml of an E. coli culture (A600=2) with the NucleoSpin 96 Plasmid Transfection-grade kit and a 96-well format kit from Competitor Z. DNA purity was assessed by absorption readings. An A_260/A280 ratio of 1.8 was obtained for plasmid DNA isolated with the NucleoSpin 96 Plasmid Transfection-grade kit, indicating the absence of contaminating compounds (e.g., RNA or proteins). Back The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields. High-copy plasmid DNA was isolated from 3 ml of an E. coli culture with the NucleoSpin 96 Plasmid Transfection-grade kit, and the eluates were loaded on a 1% TAE agarose gel and stained with ethidium bromide. Lane M: 1 kb ladder (Thermo Scientific). Lanes 1–23: Eluted plasmid samples. Back The NucleoSpin Plasmid 96 Transfection-grade procedure The NucleoSpin Plasmid 96 Transfection-grade procedure. Back 740492.4: NucleoSpin 96 Plasmid Transfection-grade Core Kit

Tips for successully using transfection reagents

Tips for a successful transfection

This guide will help you select the right reagent and find useful tips for designing a successful transfection experiment. Tips include: how to plate your cells at the right density, how to make sure you are using plasmid DNA of sufficiently high quality, and how to assess transfection efficiency.

Transfection tips Plasmid transfection reagents

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Tech Note

At-a-glance

Cell viability

Choose the right kit

Order Information

Notice to purchaser

Different plasmid purification technologies yield endotoxin levels appropriate for individual applications

Compatibility of eluted DNA with transfection host cells

The NucleoSpin Plasmid Transfection-grade procedure

740490.50: NucleoSpin Plasmid Transfection-grade

Notice to purchaser

740490.250: NucleoSpin Plasmid Transfection-grade

Different plasmid purification technologies yield endotoxin levels appropriate for individual applications

Compatibility of eluted DNA with transfection host cells

The NucleoSpin Plasmid Transfection-grade procedure

Notice to purchaser

740491.1: NucleoSpin 96 Plasmid Transfection-grade

NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor

NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor

The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields

The NucleoSpin Plasmid 96 Transfection-grade procedure

Notice to purchaser

740491.24: NucleoSpin 96 Plasmid Transfection-grade

NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor

NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor

The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields

The NucleoSpin Plasmid 96 Transfection-grade procedure

Notice to purchaser

NucleoSpin 96 Plasmid Transfection-grade provides higher yields of transfection-grade plasmid DNA than a competitor

NucleoSpin 96 Plasmid Transfection-grade yields higher-quality plasmid DNA than a competitor

The NucleoSpin 96 Plasmid Transfection-grade kit provides reproducible yields

The NucleoSpin Plasmid 96 Transfection-grade procedure

740492.4: NucleoSpin 96 Plasmid Transfection-grade Core Kit