|Technology||Silica-membrane and endotoxin-reduction technology|
|Format||Mini spin columns/96-well plates|
|Endotoxin content||<50 EU/μg DNA (EU = Endotoxin Units)|
|Sample material||Up to 5-ml bacterial culture|
|Vector size||<15 kbp|
|Typical yield||5–20 μg|
|Elution volume||30–200 μl|
|Preparation time||14 min/6 preps or 45 min/96 preps|
|Binding capacity||35 μg|
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NucleoSpin Plasmid Transfection-grade
Endotoxins are copurified during plasmid preparations from E. coli lysates and interfere with eukaryotic cell viability, making endotoxin reduction an essential step prior to cell transfection. NucleoSpin Plasmid Transfection-grade incorporates an innovative technology to reduce endotoxins from bacterial lysates compared to standard plasmid miniprep kits. The NucleoSpin Plasmid Transfection-grade and NucleoSpin 96 Plasmid Transfection-grade kits are designed for rapid, manual or automated, small-scale purification of transfection-grade plasmid DNA from E. coli.
If your cells are especially sensitive to endotoxin, see also Nucleobond Xtra EF products for the lowest possible endotoxin levels in your plasmid preps.
Tips for a successful transfection
This guide will help you select the right reagent and find useful tips for designing a successful transfection experiment. Tips include: how to plate your cells at the right density, how to make sure you are using plasmid DNA of sufficiently high quality, and how to assess transfection efficiency.Transfection tips Plasmid transfection reagents
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