*For human stool samples, approximately 200 mg should be used. For animal stool samples, depending on the species, a smaller amount of sample material may be required for optimal results.

Figure 1. NucleoSpin DNA Stool provides higher quality DNA and better yields than competitor kits. DNA was isolated from human stool samples with the NucleoSpin DNA Stool kit or with competitor products (MO, Z, Q). Panel A. DNA was extracted according to manufacturers' protocols, and 5% of the eluates were subjected to gel electrophoresis. Panel B. The yield and quality of the DNA isolated from the samples shown in Panel A were assessed using UV absorption measurements. The genomic DNA isolated with the NucleoSpin DNA Stool kit showed superior yields and quality.

Figure 2. PCR inhibitors were efficiently removed from stool samples derived from different species using NucleoSpin DNA Stool. DNA was isolated from human and animal feces samples with the NucleoSpin DNA Stool kit. 5 μl of undiluted eluate served as the template for the amplification of a 1.5-kb fragment from the bacterial 16S rRNA gene in an end-point PCR reaction (35 cycles). This fragment was successfully amplified from the undiluted DNA extracted from the various sample sources using the NucleoSpin DNA Stool kit, indicating the successful removal of PCR inhibitors. Lane 1. GeneRuler 1 kb DNA Ladder (Thermo Fisher Scientific). Lane 2. Feline. Lane 3. Sheep. Lane 4. Rabbit. Lane 5. Mouse. Lane 6. Human.

Figure 3. The NucleoSpin DNA Stool procedure. Samples can be disrupted using MN Bead Tubes and an MN Bead Tube Holder, or common disruption devices such as a swing mill (see user manuals for details on MN Bead Tube processing and procedures).