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Overviews RNA purification from stool
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Product overview

Genomic DNA from stool samples

  • Suitable for any stool sample—high-quality DNA from the stool of carnivores, omnivores, and herbivores
  • Includes MN Tubes for efficient lysis—compatible with most common disruption devices
  • NucleoSpin Inhibitor Removal Columns ensure superior DNA purity

NucleoSpin DNA Stool is a kit for purification of genomic DNA from bacterial and epithelial cells found in stool samples. The isolated DNA is used for analysis of the microbiome or genotyping of the donor.

At-a-glance Application data Protocol

At-a-glance  

Technology Silica membrane technology combined with MN Bead Tubes Type A
Format Mini spin columns
Starting material Approximately 200 mg (180–220 mg)* stool samples (fresh or frozen)
Fragment size 200 bp–approximately 50 kb
Typical yield Depends on sample type, quality, and water content
A260/A280 1.7–1.9
Elution volume 30–100 µl
Preparation time 60 min/10 preps
Binding capacity 50 µg

*For human stool samples, approximately 200 mg should be used. For animal stool samples, depending on the species, a smaller amount of sample material may be required for optimal results.

Application data  

High genomic DNA yield and purity from human stool samples

Higher quality DNA and better yields with NucleoSpin DNA Stool

Figure 1. NucleoSpin DNA Stool provides higher quality DNA and better yields than competitor kits. DNA was isolated from human stool samples with the NucleoSpin DNA Stool kit or with competitor products (MO, Z, Q). Panel A. DNA was extracted according to manufacturers' protocols, and 5% of the eluates were subjected to gel electrophoresis. Panel B. The yield and quality of the DNA isolated from the samples shown in Panel A were assessed using UV absorption measurements. The genomic DNA isolated with the NucleoSpin DNA Stool kit showed superior yields and quality.

Efficient removal of PCR inhibitors from various sample sources

Efficient removal of PCR inhibitors from different stool sample sources

Figure 2. PCR inhibitors were efficiently removed from stool samples derived from different species using NucleoSpin DNA Stool. DNA was isolated from human and animal feces samples with the NucleoSpin DNA Stool kit. 5 μl of undiluted eluate served as the template for the amplification of a 1.5-kb fragment from the bacterial 16S rRNA gene in an end-point PCR reaction (35 cycles). This fragment was successfully amplified from the undiluted DNA extracted from the various sample sources using the NucleoSpin DNA Stool kit, indicating the successful removal of PCR inhibitors. Lane 1. GeneRuler 1 kb DNA Ladder (Thermo Fisher Scientific). Lane 2. Feline. Lane 3. Sheep. Lane 4. Rabbit. Lane 5. Mouse. Lane 6. Human.

Protocol  

The NucleoSpin DNA Stool kit utilizes MN Bead Tubes Type A (ceramic beads; included in kit) in combination with a common disruption device, e.g., an MN Bead Tube Holder, or a swing mill to lyse different cell types and tissues within a single procedure. Alternative MN Bead Tubes can be ordered separately if needed. NucleoSpin Inhibitor Removal Columns are included for the convenient elimination of contaminants.

DNA isolation using NucleoSpin DNA Stool

Figure 3. The NucleoSpin DNA Stool procedure. Samples can be disrupted using MN Bead Tubes and an MN Bead Tube Holder, or common disruption devices such as a swing mill (see user manuals for details on MN Bead Tube processing and procedures).

Related Products

Cat. # Product Size License Quantity Details
740472.50 NucleoSpin® DNA Stool 50 Preps USD $299.00

Miniprep kit (50 preps) for the purification of genomic DNA from bacterial and epithelial cells in stool samples. The isolated DNA can be used for analysis of the microbiome or genotyping of the donor. The NucleoSpin DNA Stool kit utilizes MN Bead Tubes Type A (ceramic beads; included in kit) in combination with a common disruption device, e.g., the MN Bead Tube Holder (Cat. # 740469), or a swing mill to lyse different cell types and tissues within a single procedure. MN Bead Tubes Type A (Cat. # 740786.50) can also be ordered separately if needed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Back

740472.50: NucleoSpin DNA Stool

740472.50: NucleoSpin DNA Stool

Required Products

Cat. # Product Size Price License Quantity Details
740469 MN Bead Tube Holder Each USD $41.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

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Back

740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740472.10 NucleoSpin® DNA Stool 10 Preps USD $78.00

Miniprep kit (10 preps) for the purification of genomic DNA from bacterial and epithelial cells in stool samples. The isolated DNA can be used for analysis of the microbiome or genotyping of the donor. The NucleoSpin DNA Stool kit utilizes MN Bead Tubes Type A (ceramic beads; included in kit) in combination with a common disruption device, e.g., the MN Bead Tube Holder (Cat. # 740469), or a swing mill to lyse different cell types and tissues within a single procedure. MN Bead Tubes Type A (Cat. # 740786.50) can also be ordered separately if needed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

740472.10: NucleoSpin DNA Stool

740472.10: NucleoSpin DNA Stool
740472.250 NucleoSpin® DNA Stool 250 Preps USD $1330.00

Miniprep kit (250 preps) for the purification of genomic DNA from bacterial and epithelial cells in stool samples. The isolated DNA can be used for analysis of the microbiome or genotyping of the donor. The NucleoSpin DNA Stool kit utilizes MN Bead Tubes Type A (ceramic beads; included in kit) in combination with a common disruption device, e.g., the MN Bead Tube Holder (Cat. # 740469), or a swing mill to lyse different cell types and tissues within a single procedure. MN Bead Tubes Type A (Cat. # 740786.50) can also be ordered separately if needed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

740472.250: NucleoSpin DNA Stool

740472.250: NucleoSpin DNA Stool
740786.50 MN Bead Tubes Type A 50 Preps USD $125.00

Pack of fifty 2-ml tubes containing ceramic beads for sample homogenization. Use in combination with the MN Bead Tube Holder (Cat. # 740469) and NucleoSpin Soil (e.g., Cat. # 740780.50) or Nucleospin Stool (Cat. # 740472.50), miniprep kits for isolation of total DNA from soil, sediments, or stool samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

740786.50: NucleoSpin Bead Tubes Type A

740786.50: NucleoSpin Bead Tubes Type A

Required Products

Cat. # Product Size Price License Quantity Details
740469 MN Bead Tube Holder Each USD $41.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

You May Also Like Image Data

Back

740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740469 MN Bead Tube Holder Each USD $41.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

You May Also Like Image Data

Back

740469: MN Bead Tube Holder

740469: MN Bead Tube Holder


Purify DNA from small samples like microdissected tissue, small blood samples, or small amounts of cells.

Designed for extra small samples

Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.

Learn more All genomic DNA kits

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  • Try BcaBEST DNA Polymerase ver.2.0
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  • Baculovirus titration kits early access program
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