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Home › Learning centers › Nucleic acid purification › RNA purification › NucleoSpin miRNA Plasma

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Tech Note

NucleoSpin miRNA Plasma

Reliable miRNA isolation—NucleoSpin miRNA Plasma

  • Efficient isolation of small RNA from plasma or serum samples—superior recovery for small RNA
  • Increased sensitivity in downstream applications
  • Optional on-column DNA digestion—rDNase included
  • Optional DNA isolation—genomic DNA (<1,000 bp) can be isolated from plasma/serum by omitting optional DNA digestion
  • Simple and fast procedure—without phenol/chloroform
Introduction Results Methods

Introduction  

The NucleoSpin miRNA Plasma procedure is a simple and fast method to isolate small RNA >15 nt, such as micro RNA (miRNA), from serum or plasma. The DNA can be isolated as well or digested on-column to eliminate interference of miRNA-encoding DNA with downstream applications.

Product at a glance

Results  

Higher yield, higher quality, quicker procedure

The performance of NucleoSpin miRNA Plasma was compared with competitor kits (below). MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 μl of human blood plasma. Competitor Q: sample volumes of 1,000 μl and 200 μl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to Q´s supplementary protocols. Competitor A: 300 μl of plasma was processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).

Higher miRNA yields

Purified miRNA (2 μl of each eluate) was used as template in quantitative realtime RT-PCR targeting miR-16 miRNA (Applied Biosystems, TaqMan MicroRNA RT Kit, hsa-miR-16 MicroRNA Assay). The results below show that CT values are lowest for NucleoSpin miRNA Plasma, indicating highest miRNA yields. As a result, NucleoSpin miRNA Plasma shows superior performance with or without optional DNase digestion.

Higher miRNA quality

Residual genomic DNA was estimated by qPCR of a 102-bp fragment of the elongation factor 1 gene using the 2x DyNAmo Capillary Master Mix (Finnzymes). There was no genomic DNA background detectable when performing the optional DNase digestion of the NucleoSpin miRNA Plasma kit. The results show that CT values are very high for NucleoSpin miRNA Plasma, indicating very low gDNA background.

Quicker procedure

By performing 10 preparations each, the quickest results were obtained by NucleoSpin miRNA Plasma both with and without rDNase digestion.

Consistent scaleup of sample volume

The standard sample volume of 300 μl can be scaled up to 900 μl by multiple loading. Purified miRNA was used as template in quantitative real-time RT-PCR for miR-16 miRNA (Applied Biosystems, TaqMan MicroRNA RT Kit, hsa-miR-16 MicroRNA Assay). The results show that CT values decrease with increasing sample volume, indicating a consistent rise in miRNA yields.

Methods  

Related products

Cat. # Product Size Price License Quantity Details
740981.10 NucleoSpin® miRNA Plasma 10 Preps $75.20

NucleoSpin miRNA Plasma (10) 10 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase EQ Excepted Quantities or

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits
Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits. MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 µl human blood plasma. Competitor Q: sample volumes of 1000 µl and 200 µl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to competitor Q`s supplementary protocols. Competitor A: 300 µl plasma were processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).When each kit was used to perform 10 preps, the fastest results were obtained using NucleoSpin miRNA Plasma, both with and without rDNase digestion.

Back

NucleoSpin miRNA Plasma procedure

NucleoSpin miRNA Plasma procedure
NucleoSpin miRNA Plasma procedure.
740981.250 NucleoSpin® miRNA Plasma 250 Preps $1300.00

NucleoSpin miRNA Plasma (250) 250 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits
Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits. MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 µl human blood plasma. Competitor Q: sample volumes of 1000 µl and 200 µl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to competitor Q`s supplementary protocols. Competitor A: 300 µl plasma were processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).When each kit was used to perform 10 preps, the fastest results were obtained using NucleoSpin miRNA Plasma, both with and without rDNase digestion.

Back

NucleoSpin miRNA Plasma procedure

NucleoSpin miRNA Plasma procedure
NucleoSpin miRNA Plasma procedure.
740981.50 NucleoSpin® miRNA Plasma 50 Preps $300.00

NucleoSpin miRNA Plasma (50) 50 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase EQ Excepted Quantities or

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits
Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits. MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 µl human blood plasma. Competitor Q: sample volumes of 1000 µl and 200 µl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to competitor Q`s supplementary protocols. Competitor A: 300 µl plasma were processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).When each kit was used to perform 10 preps, the fastest results were obtained using NucleoSpin miRNA Plasma, both with and without rDNase digestion.

Back

NucleoSpin miRNA Plasma procedure

NucleoSpin miRNA Plasma procedure
NucleoSpin miRNA Plasma procedure.

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