NucleoSpin miRNA Plasma

Home › Learning centers › Nucleic acid purification › RNA purification › NucleoSpin miRNA Plasma

Nucleic acid purification

New products

Need help?

Tech Note

NucleoSpin miRNA Plasma

Reliable miRNA isolation—NucleoSpin miRNA Plasma

Efficient isolation of small RNA from plasma or serum samples—superior recovery for small RNA
Increased sensitivity in downstream applications
Optional on-column DNA digestion—rDNase included
Optional DNA isolation—genomic DNA (<1,000 bp) can be isolated from plasma/serum by omitting optional DNA digestion
Simple and fast procedure—without phenol/chloroform

Introduction Results Methods

Introduction

The NucleoSpin miRNA Plasma procedure is a simple and fast method to isolate small RNA >15 nt, such as micro RNA (miRNA), from serum or plasma. The DNA can be isolated as well or digested on-column to eliminate interference of miRNA-encoding DNA with downstream applications.

Product at a glance

Results

Higher yield, higher quality, quicker procedure

The performance of NucleoSpin miRNA Plasma was compared with competitor kits (below). MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 μl of human blood plasma. Competitor Q: sample volumes of 1,000 μl and 200 μl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to Q´s supplementary protocols. Competitor A: 300 μl of plasma was processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).

Higher miRNA yields

Purified miRNA (2 μl of each eluate) was used as template in quantitative realtime RT-PCR targeting miR-16 miRNA (Applied Biosystems, TaqMan MicroRNA RT Kit, hsa-miR-16 MicroRNA Assay). The results below show that CT values are lowest for NucleoSpin miRNA Plasma, indicating highest miRNA yields. As a result, NucleoSpin miRNA Plasma shows superior performance with or without optional DNase digestion.

Higher miRNA quality

Residual genomic DNA was estimated by qPCR of a 102-bp fragment of the elongation factor 1 gene using the 2x DyNAmo Capillary Master Mix (Finnzymes). There was no genomic DNA background detectable when performing the optional DNase digestion of the NucleoSpin miRNA Plasma kit. The results show that CT values are very high for NucleoSpin miRNA Plasma, indicating very low gDNA background.

Quicker procedure

By performing 10 preparations each, the quickest results were obtained by NucleoSpin miRNA Plasma both with and without rDNase digestion.

Consistent scaleup of sample volume

The standard sample volume of 300 μl can be scaled up to 900 μl by multiple loading. Purified miRNA was used as template in quantitative real-time RT-PCR for miR-16 miRNA (Applied Biosystems, TaqMan MicroRNA RT Kit, hsa-miR-16 MicroRNA Assay). The results show that CT values decrease with increasing sample volume, indicating a consistent rise in miRNA yields.

Methods

Related products

Cat. #	Product	Size	Price
740981.10	NucleoSpin® miRNA Plasma	10 Preps	$74.00
NucleoSpin miRNA Plasma (10) 10 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase EQ Excepted Quantities or Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits. MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 µl human blood plasma. Competitor Q: sample volumes of 1000 µl and 200 µl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to competitor Q`s supplementary protocols. Competitor A: 300 µl plasma were processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).When each kit was used to perform 10 preps, the fastest results were obtained using NucleoSpin miRNA Plasma, both with and without rDNase digestion. Back NucleoSpin miRNA Plasma procedure NucleoSpin miRNA Plasma procedure.
740981.250	NucleoSpin® miRNA Plasma	250 Preps	$1278.00
NucleoSpin miRNA Plasma (250) 250 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits. MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 µl human blood plasma. Competitor Q: sample volumes of 1000 µl and 200 µl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to competitor Q`s supplementary protocols. Competitor A: 300 µl plasma were processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).When each kit was used to perform 10 preps, the fastest results were obtained using NucleoSpin miRNA Plasma, both with and without rDNase digestion. Back NucleoSpin miRNA Plasma procedure NucleoSpin miRNA Plasma procedure.
740981.50	NucleoSpin® miRNA Plasma	50 Preps	$295.00
NucleoSpin miRNA Plasma (50) 50 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase EQ Excepted Quantities or Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits. MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 µl human blood plasma. Competitor Q: sample volumes of 1000 µl and 200 µl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to competitor Q`s supplementary protocols. Competitor A: 300 µl plasma were processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).When each kit was used to perform 10 preps, the fastest results were obtained using NucleoSpin miRNA Plasma, both with and without rDNase digestion. Back NucleoSpin miRNA Plasma procedure NucleoSpin miRNA Plasma procedure.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2018 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.