| Technology | Silica membrane |
| Format | Two columns: XS column for DNA removal XS column for RNA isolation |
| Starting material | Cultured cells (1-105) Human/animal tissue (<5 mg) |
| Typical yield | HeLa cells (105): 0.5–2 μg HeLa cells (101): 0.05–0.2 ng Mouse liver (0.5 μg): 2.5–8 ng Mouse brain (0.5 μg): 0.1–0.5 ng |
| Elution volume | 5–30 μl |
| A260/A280 | 1.9–2.2 |
| Preparation time | 18 min/6 preps |
| Binding capacity | 110 μg |
Tech Note
Rapid purification of total RNA from extra small samples
NucleoSpin RNA Plus XS enables efficient isolation of high-quality RNA from extra small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS allows enzyme-free DNA removal, saving 15 min of prep time compared to classical DNA digestions. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that omits reducing agents such as ß-mercaptoethanol or TCEP, avoiding unpleasant smells and health hazards.
- Developed for extra small samples down to single cell analysis
- DNA removal column eliminates the need for time-consuming DNA digestion
- Novel lysis buffer contains no ß-mercaptoethanol or TCEP
- RNA isolation from small amounts of cultured cells, human/animal tissue
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays, RNA-sequencing
At-a-glance
Column design
Figure 1. The NucleoSpin gDNA Removal Column XS (left) allows DNA removal without digestion, while the NucleoSpin RNA Plus XS Column (right) allows elution volumes down to 5 μl.
Figure 2. Design of the NucleoSpin RNA Plus XS column. The innovative funnel-shaped thrust ring allows standard miniprep spin columns to hold a silica membrane of very small diameter. The small surface of the NucleoSpin RNA Plus XS membrane provides efficient elution in only 5–30 μl, resulting in highly concentrated RNA that is ready for all typical downstream applications.
Sensitivity down to single cell analysis
Figure 3. NucleoSpin RNA Plus XS enables reliable isolation of high-quality RNA from extra small samples down to a single cell. RNA was isolated from HeLa cells (104 to 101 cells) using NucleoSpin RNA Plus XS (MN) and four competitor products (Q+, Q, I, Z), and analyzed by qRT-PCR (top panel). RNA was isolated from HeLa cells (105 cells to 1 cell) using NucleoSpin RNA Plus XS and analyzed by qRT-PCR (bottom panel).
Reliable DNA removal
Figure 4. Reliable genomic DNA removal in comparison to competitor products. RNA was isolated from HeLa cells (104 to 101 cells) in triplicates using NucleoSpin RNA Plus XS (MN) and four leading competitor products (Q+, Q, I, Z). Residual genomic DNA was measured by qPCR.
Reproducible yield
Figure 5. NucleoSpin RNA Plus XS combines reliable high sensitivity with reproducible high yields. Total RNA was extracted from HeLa cells (105 cells to 1 cell) in triplicates using NucleoSpin RNA Plus XS. Elution was performed with 10 μl, of which 2 μl were subjected to one-tube, one-step qRT-PCR. NucleoSpin RNA Plus XS allows efficient isolation from HeLa cell sample amounts over several orders of magnitude, down to 1 cell.
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