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Tech Note

Rapid purification of total RNA from extra small samples

NucleoSpin RNA Plus XS enables efficient isolation of high-quality RNA from extra small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS allows enzyme-free DNA removal, saving 15 min of prep time compared to classical DNA digestions. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that omits reducing agents such as ß-mercaptoethanol or TCEP, avoiding unpleasant smells and health hazards.

  • Developed for extra small samples down to single cell analysis
  • DNA removal column eliminates the need for time-consuming DNA digestion
  • Novel lysis buffer contains no ß-mercaptoethanol or TCEP
  • RNA isolation from small amounts of cultured cells, human/animal tissue
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays, RNA-sequencing
At-a-glance Column design Sensitivity down to single cell analysis Reliable DNA removal Reproducible yield

At-a-glance  

Technology Silica membrane
Format Two columns:
XS column for DNA removal
XS column for RNA isolation
Starting material Cultured cells (1-105)
Human/animal tissue (<5 mg)
Typical yield HeLa cells (105): 0.5–2 μg
HeLa cells (101): 0.05–0.2 ng
Mouse liver (0.5 μg): 2.5–8 ng
Mouse brain (0.5 μg): 0.1–0.5 ng
Elution volume 5–30 μl
A260/A280 1.9–2.2
Preparation time 18 min/6 preps
Binding capacity 110 μg

Column design  

NucleoSpin RNA Plus XS ColumnNucleoSpin gDNA Removal Column XS

Figure 1. The NucleoSpin gDNA Removal Column XS (left) allows DNA removal without digestion, while the NucleoSpin RNA Plus XS Column (right) allows elution volumes down to 5 μl.

 NucleoSpin RNA Plus XS membrane

Figure 2. Design of the NucleoSpin RNA Plus XS column. The innovative funnel-shaped thrust ring allows standard miniprep spin columns to hold a silica membrane of very small diameter. The small surface of the NucleoSpin RNA Plus XS membrane provides efficient elution in only 5–30 μl, resulting in highly concentrated RNA that is ready for all typical downstream applications.

Sensitivity down to single cell analysis  

RNA was isolated from HeLa cells (104 to 101 cells) with NucleoSpin RNA Plus XS (MN) and four competitor products    

Figure 3. NucleoSpin RNA Plus XS enables reliable isolation of high-quality RNA from extra small samples down to a single cell. RNA was isolated from HeLa cells (104 to 101 cells) using NucleoSpin RNA Plus XS (MN) and four competitor products (Q+, Q, I, Z), and analyzed by qRT-PCR (top panel). RNA was isolated from HeLa cells (105 cells to 1 cell) using NucleoSpin RNA Plus XS and analyzed by qRT-PCR (bottom panel).

Reliable DNA removal  

Reliable genomic DNA removal in comparison to competitor products.

Figure 4. Reliable genomic DNA removal in comparison to competitor products. RNA was isolated from HeLa cells (104 to 101 cells) in triplicates using NucleoSpin RNA Plus XS (MN) and four leading competitor products (Q+, Q, I, Z). Residual genomic DNA was measured by qPCR.

Reproducible yield  

NucleoSpin RNA Plus XS combines reliable high sensitivity with reproducible high yields.

Figure 5. NucleoSpin RNA Plus XS combines reliable high sensitivity with reproducible high yields. Total RNA was extracted from HeLa cells (105 cells to 1 cell) in triplicates using NucleoSpin RNA Plus XS. Elution was performed with 10 μl, of which 2 μl were subjected to one-tube, one-step qRT-PCR. NucleoSpin RNA Plus XS allows efficient isolation from HeLa cell sample amounts over several orders of magnitude, down to 1 cell.

Related Products

Cat. # Product Size License Quantity Details
740990.250 NucleoSpin® RNA Plus XS 250 Preps USD $1905.00

The NucleoSpin RNA Plus XS kit (250 preps) is optimized for efficient isolation of high-quality RNA from extra-small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS provides enzyme-free DNA removal, saving 15 min in prep time compared to standard DNA digestion methods. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that eliminates the need for reducing agents such as beta-mercaptoethanol or TCEP, avoiding unpleasant odors and health concerns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of HeLa cells using NucleoSpin RNA Plus XS or NucleoSpin RNA XS, respectively, by following the standard protocol for each kit (the former employs a gDNA removal column while the latter includes an on-column DNase digestion step). The amount of residual genomic DNA in the purified sample varied depending on the input amount, but for the input range of 10–10,000 cells, the NucleoSpin RNA Plus XS kit provided more efficient removal of genomic DNA than the NucleoSpin RNA XS kit.

Back

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of mouse liver tissue using NucleoSpin RNA Plus XS or kits from Competitor Q/Q+, I, and Z, respectively. Yields of total RNA obtained with NucleoSpin RNA Plus XS were greater than or comparable to yields obtained with each competitor kit for each of the input amounts processed.

Back

740990.250: NucleoSpin RNA Plus XS

740990.250: NucleoSpin RNA Plus XS
740990.50 NucleoSpin® RNA Plus XS 50 Preps USD $431.00

The NucleoSpin RNA Plus XS kit (50 preps) is optimized for efficient isolation of high-quality RNA from extra-small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS provides enzyme-free DNA removal, saving 15 min in prep time compared to standard DNA digestion methods. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that eliminates the need for reducing agents such as beta-mercaptoethanol or TCEP, avoiding unpleasant odors and health concerns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of HeLa cells using NucleoSpin RNA Plus XS or NucleoSpin RNA XS, respectively, by following the standard protocol for each kit (the former employs a gDNA removal column while the latter includes an on-column DNase digestion step). The amount of residual genomic DNA in the purified sample varied depending on the input amount, but for the input range of 10–10,000 cells, the NucleoSpin RNA Plus XS kit provided more efficient removal of genomic DNA than the NucleoSpin RNA XS kit.

Back

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of mouse liver tissue using NucleoSpin RNA Plus XS or kits from Competitor Q/Q+, I, and Z, respectively. Yields of total RNA obtained with NucleoSpin RNA Plus XS were greater than or comparable to yields obtained with each competitor kit for each of the input amounts processed.

Back

740990.50: NucleoSpin RNA Plus XS

740990.50: NucleoSpin RNA Plus XS
740990.10 NucleoSpin® RNA Plus XS 10 Preps USD $101.00

The NucleoSpin RNA Plus XS kit (10 preps) is optimized for efficient isolation of high-quality RNA from extra-small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS provides enzyme-free DNA removal, saving 15 min in prep time compared to standard DNA digestion methods. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that eliminates the need for reducing agents such as beta-mercaptoethanol or TCEP, avoiding unpleasant odors and health concerns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of HeLa cells using NucleoSpin RNA Plus XS or NucleoSpin RNA XS, respectively, by following the standard protocol for each kit (the former employs a gDNA removal column while the latter includes an on-column DNase digestion step). The amount of residual genomic DNA in the purified sample varied depending on the input amount, but for the input range of 10–10,000 cells, the NucleoSpin RNA Plus XS kit provided more efficient removal of genomic DNA than the NucleoSpin RNA XS kit.

Back

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of mouse liver tissue using NucleoSpin RNA Plus XS or kits from Competitor Q/Q+, I, and Z, respectively. Yields of total RNA obtained with NucleoSpin RNA Plus XS were greater than or comparable to yields obtained with each competitor kit for each of the input amounts processed.

Back

740990.10: NucleoSpin RNA Plus XS

740990.10: NucleoSpin RNA Plus XS

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