Technology | Silica membrane |
Format | Two columns: XS column for DNA removal XS column for RNA isolation |
Starting material | Cultured cells (1-105) Human/animal tissue (<5 mg) |
Typical yield | HeLa cells (105): 0.5–2 μg HeLa cells (101): 0.05–0.2 ng Mouse liver (0.5 μg): 2.5–8 ng Mouse brain (0.5 μg): 0.1–0.5 ng |
Elution volume | 5–30 μl |
A260/A280 | 1.9–2.2 |
Preparation time | 18 min/6 preps |
Binding capacity | 110 μg |
Tech Note
Rapid purification of total RNA from extra small samples
NucleoSpin RNA Plus XS enables efficient isolation of high-quality RNA from extra small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS allows enzyme-free DNA removal, saving 15 min of prep time compared to classical DNA digestions. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that omits reducing agents such as ß-mercaptoethanol or TCEP, avoiding unpleasant smells and health hazards.
- Developed for extra small samples down to single cell analysis
- DNA removal column eliminates the need for time-consuming DNA digestion
- Novel lysis buffer contains no ß-mercaptoethanol or TCEP
- RNA isolation from small amounts of cultured cells, human/animal tissue
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays, RNA-sequencing
At-a-glance
Column design
Sensitivity down to single cell analysis
Reliable DNA removal
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