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Overviews Single-prep DNA purification from soil
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NucleoBond RNA Soil product page NucleoBond RNA Soil product page
Overviews Single-prep DNA purification from soil
Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Product overview

NucleoBond RNA Soil and NucleoBond RNA Soil Mini

Metagenomic analyses of soil and sediment samples are often hampered by insufficient lysis of microbial cells and the relatively low amounts of RNA present in these sample types. Developed with these challenges in mind, the NucleoBond RNA Soil kit accommodates sample inputs as large as 2 g and employs ceramic beads (MN Bead Tubes Type A) to aid in processing of hard-to-lyse microorganisms, typically yielding 1–10 µg of high-quality RNA per sample. For greater experimental flexibility, NucleoBond RNA Soil Mini provides the same purification technology in a smaller format.

  • Anion exchange technology to optimize RNA yield and purity—suitable for metagenomic studies
  • Combination of mechanical homogenization and chemical lysis allows processing of large sample amounts
  • Parallel preparation of RNA and DNA in one hour when combined with the DNA Set for NucleoBond RNA Soil or DNA Set for NucleoBond RNA Soil Mini

At-a-glance High yield and quality Parallel DNA isolation Mini format available Protocol

At-a-glance  

NucleoBond RNA Soil NucleoBond RNA Soil Mini
Technology Anion exchange chromatography
Format Gravity flow columns combined with MN Bead Tubes Type A
Starting material <2 g of soil 0.25–0.5 g soil
Fragment size >100 nt
Typical yield 1–10 µg 0.25–2.5 µg
A260/A280 1.7–2.1 1.5–2.0
A260/A230 1.8–2.5 —
Typical RIN (RNA integrity number) >8.5 >7.0
Elution volume 100 µl 50–100 µl
Preparation time 60 min/6 preps 60 min/12 preps
Binding capacity 600 µg 30 µg

High yield and quality  

High RNA yield with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, forest soil) were purified in duplicate with NucleoBond RNA Soil (MN) using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 μl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit.

High RNA quality with NucleoBond RNA Soil

Superior RT-PCR performance with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil (MN) and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower CP values relative to corresponding samples prepared using kit "Q".

Parallel DNA isolation  

efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil (MN) following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

Mini format available  

Yields sufficient RNA for qPCR analysis

NucleoBond RNA Soil Mini yields sufficient RNA for qPCR analysis. Two different soil samples were purified in duplicate using NucleoBond RNA Soil Mini in comparison with NucleoBond RNA Soil. RNA was eluted in 100 µl and analyzed photometrically, and 15 µl of each eluate was used for gel electrophoresis (1% TAE). RNA yields obtained with NucleoBond RNA Soil Mini were proportional to the reduced input amount for this format relative to yields obtained with NucleoBond RNA Soil, and sufficient for downstream applications such as qRT-PCR.

Efficient parallel DNA isolation

NucleoBond RNA Soil Mini provides efficient parallel DNA isolation. Two different soil samples were purified in duplicate using NucleoBond RNA Soil Mini in comparison with NucleoBond RNA Soil. DNA was eluted in 100 µl and measured photometrically, and 15 µl of each eluate was applied to a 1% TAE gel for electrophoresis analysis. DNA yields obtained using NucleoBond RNA Soil Mini with the DNA Set for NucleoBond RNA Soil Mini were proportional to the reduced input amount for this format relative to yields obtained with NucleoBond RNA Soil and the corresponding DNA Set for NucleoBond RNA Soil.

Protocol  

NucleoBond RNA Soil procedure

Protocol overview for NucleoBond RNA Soil*.

Organisms in each soil sample are lysed by bead beating in the presence of Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol. Buffer OPT can be included in the lysis step to reduce the adsorption of nucleic acids to clay and mineral soil components but will also increase contamination with humic acids (if present). Performance cannot be predicted and depends on the soil composition, but as a rule of thumb a sample with a high content of organic and humic components (e.g., forest soil) should be lysed without Buffer OPT while predominantly mineral soils (e.g., river sediments and clay) should be lysed with Buffer OPT. Unlysed components are sedimented by centrifugation.

In the protocol for NucleoBond RNA Soil, the supernatants of the four bead tubes are transferred and combined into one fresh 15-ml tube and mixed with Binding Buffer E2. The incubation and centrifugation steps that follow can be used to prepare the NucleoBond RNA Columns. The columns including filters are fixed with the supplied Plastic Washers on top of a 50-ml tube or laboratory flask or any NucleoBond Rack. Filters and columns are equilibrated by Buffer EQU to pre-wet the filters and to prepare the anion exchange chromatography columns. Once the columns are equilibrated, the clear supernatant of the centrifuged samples is loaded onto the filters in the purification columns. Undissolved particles and some of the soluble macro molecules will be held back by the filters. Wash Buffer E3 is used to flush the filter columns and to wash the nucleic acids from the filters onto the column matrix. Nucleic acids and soluble polyanionic molecules including some fraction of humic substances (if present) will bind to the anion exchange surface. A brown layer might be visible on top of the silica matrix. After the washing step, the filter is removed, and the anion exchange column is washed with Buffer E4 to remove contaminants. RNA is eluted by Buffer ERNA. If isolation of RNA and DNA is desired, DNA can be eluted with Buffer EDNA, included with the DNA Set for NucleoBond RNA Soil (Cat. # 740141.20 available separately; see below) from the same column. Isopropanol is added to the eluates and the mixture is bound to NucleoSpin Finisher Columns by centrifugation. The columns are washed with Buffer E5 and dried. Samples are then eluted in RNase-free H2O.

*NucleoBond RNA Soil Mini uses a similar protocol, but on a smaller scale.

Related products

Cat. # Product Size License Quantity Details
740140.20 NucleoBond® RNA Soil 20 Preps USD $405.00

The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure.

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High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit.

Back

Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q".

Back

NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

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740140.2: NucleoBond RNA Soil

740140.2: NucleoBond RNA Soil
740141.20 DNA Set for NucleoBond® RNA Soil 20 Preps USD $80.00

DNA Set for NucleoBond RNA Soil is a 20-prep elution set designed to be used in conjunction with NucleoBond RNA Soil (Cat. # 740140.20) for parallel isolation of DNA and RNA from the same soil sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. DNA Set for NucleoBond RNA Soil typically yields 5–50 µg of pure DNA from 1–2 g of soil. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure.

Back

High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit.

Back

Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q".

Back

NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

Required Products

Cat. # Product Size Price License Quantity Details
740140.20 NucleoBond® RNA Soil 20 Preps USD $405.00

The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

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NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure.

Back

High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit.

Back

Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q".

Back

NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

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740140.2: NucleoBond RNA Soil

740140.2: NucleoBond RNA Soil
740786.50 MN Bead Tubes Type A 50 Preps USD $125.00

Pack of fifty 2-ml tubes containing ceramic beads for sample homogenization. Use in combination with the MN Bead Tube Holder (Cat. # 740469) and NucleoSpin Soil (e.g., Cat. # 740780.50) or Nucleospin Stool (Cat. # 740472.50), miniprep kits for isolation of total DNA from soil, sediments, or stool samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740786.50: NucleoSpin Bead Tubes Type A

740786.50: NucleoSpin Bead Tubes Type A

Required Products

Cat. # Product Size Price License Quantity Details
740469 MN Bead Tube Holder Each USD $41.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

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740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740469 MN Bead Tube Holder Each USD $41.00

Blue rubber foam adapter for processing MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740142.10 NucleoBond® RNA Soil Mini 10 Preps USD $161.00

The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies. including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 10 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

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NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.

740142.50 NucleoBond® RNA Soil Mini 50 Preps USD $750.00

The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

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NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.

740143.10 DNA Set for NucleoBond® RNA Soil Mini 10 Preps USD $31.00

DNA Set for NucleoBond RNA Soil Mini is a 10-prep elution set designed to be used in conjunction with NucleoBond RNA Soil Mini (Cat. # 740142.10) for parallel isolation of DNA and RNA from the same soil sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. DNA Set for NucleoBond RNA Soil Mini typically yields 1.25–12.5 µg of pure DNA from 0.25–0.5 g of soil. The kit includes sufficient quantities of reagents and materials for processing of 10 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

Required Products

Cat. # Product Size Price License Quantity Details
740142.10 NucleoBond® RNA Soil Mini 10 Preps USD $161.00

The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies. including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 10 samples.

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NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

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NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.

740143.50 DNA Set for NucleoBond® RNA Soil Mini 50 Preps USD $141.00

DNA Set for NucleoBond RNA Soil Mini is a 50-prep elution set designed to be used in conjunction with NucleoBond RNA Soil Mini (Cat. # 740142.50) for parallel isolation of DNA and RNA from the same soil sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. DNA Set for NucleoBond RNA Soil Mini typically yields 1.25–12.5 µg of pure DNA from 0.25–0.5 g of soil. The kit includes sufficient quantities of reagents and materials for processing of 50 samples.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

Required Products

Cat. # Product Size Price License Quantity Details
740142.50 NucleoBond® RNA Soil Mini 50 Preps USD $750.00

The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 50 samples.

Documents You May Also Like Image Data

Back

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

Back

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.


RNA purification mini prep kits that stop RNA degradation and prevent genomic DNA contamination

The better way to isolate RNA

Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.

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  • cDNA synthesis kits
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  • Purified total RNA and mRNA
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