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NucleoBond accessories & consumables

Accessories for Nucleobond nucleic acid purification kits

Accessories and consumables for NucleoBond anion-exchange columns: folded filters and individual columns.

Cat. #	Product	Size
740577	NucleoBond® Folded Filters XL	50 Filters	USD $57.00
NucleoBond Folded Filters XL (50) folded filters for NucleoBond AX 500 and AX 2000 Columns pack of 50 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740577: NucleoBond Folded Filters XL
740561	NucleoBond® Folded Filters	50 Filters	USD $46.00
NucleoBond Folded Filters (50) folded filters for NucleoBond AX 100 Columns pack of 50 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740561: NucleoBond Folded Filters
740553.5	NucleoBond® Bottle Top Filter Type 2	5 Filters	USD $105.00
NucleoBond Bottle Top Filter Type 2 (5) 5 NucleoBond Bottle Top Filters for use with NucleoBond AX 10000 Columns pack of 5 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoBond Bottle Top Filter Type 2—for use with NucleoBond AX 10000 cartridges NucleoBond Bottle Top Filter Type 2—for use with NucleoBond AX 10000 cartridges. Back 740553.5: NucleoBond Bottle Top Filter Type 2
740547.5	NucleoBond® Bottle Top Filter Type 1	5 Filters	USD $92.00
NucleoBond Bottle Top Filter Type 1 (5) NucleoBond Bottle Top Filters for use with NucleoBond AX 2000 Columns pack of 5 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoBond Bottle Top Filter Type 1—for use with NucleoBond AX 2000 cartridges NucleoBond Bottle Top Filter Type 1—for use with NucleoBond AX 2000 cartridges. Back 740547.5: NucleoBond Bottle Top Filter Type 1
740546	NucleoBond® AXG 500	10 Columns	USD $240.00
NucleoBond AXG 500 Columns (10) columns (no buffers provided) for the isolation of genomic DNA from cells, tissue, and bacteria. Combine NucleoBond AXG columns and the corresponding buffer set (III or IV) for highest flexibility. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740546: NucleoBond AXG 500
740545	NucleoBond® AXG 100	20 Columns	USD $214.00
NucleoBond AXG 100 Columns (20) columns (no buffers provided) for the isolation of genomic DNA from cells, tissue, and bacteria. Combine NucleoBond AXG columns and the corresponding buffer set (III or IV) for highest flexibility. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Purification of genomic DNA from enterobacteria with NucleoBond AXG 100 Purification of genomic DNA from enterobacteria with NucleoBond AXG 100. Lane 1. Molecular weight marker. Lane 2. Purified genomic DNA. Back 740545: NucleoBond AXG 100
740544	NucleoBond® AXG 20	20 Columns	USD $151.00
NucleoBond AXG 20 Columns (20) columns (no buffers provided) for the isolation of genomic DNA from cells, tissue, and bacteria. Combine NucleoBond AXG columns and the corresponding buffer set (III or IV) for highest flexibility. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740544: NucleoBond AXG 20
740534	NucleoBond® AX 10,000	5 Columns	USD $408.00
NucleoBond AX 10000 Columns (5) columns for the isolation of plasmid DNA pack of 5 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740534: NucleoBond AX 10,000
740531.50	NucleoBond® AX 500	50 Columns	USD $890.00
NucleoBond AX 500 Columns (50) columns for the isolation of plasmid DNA pack of 50 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740531.50: NucleoBond AX 500
740531	NucleoBond® AX 500	10 Columns	USD $185.00
NucleoBond AX 500 Columns (10) columns for the isolation of plasmid DNA pack of 10 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740531: NucleoBond AX 500
740525	NucleoBond® AX 2000	10 Columns	USD $387.00
NucleoBond AX 2000 Columns (10) columns for the isolation of plasmid DNA pack of 10 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740525: NucleoBond AX 2000
740521.100	NucleoBond® AX 100	100 Columns	USD $820.00
NucleoBond AX 100 Columns (100) columns for the isolation of plasmid DNA pack of 100 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740521.100: NucleoBond AX 100
740521	NucleoBond® AX 100	20 Columns	USD $192.00
NucleoBond AX 100 Columns (20) columns for the isolation of plasmid DNA pack of 20 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740521: NucleoBond AX 100
740520.20	NucleoBond® Finalizer Plus	20 Finalizers	USD $87.00
NucleoBond Finalizer Plus (20) filters for plasmid DNA precipitation, to be used with NucleoBond PC 100, PC 500, and Xtra Midi(EF) kits - 20 NucleoBond Finalizers, 20 syringes of 30 mL, and 20 syringes of 1 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740520.20: NucleoBond Finalizer Plus
740519.20	NucleoBond® Finalizer	20 Finalizers	USD $48.00
NucleoBond Finalizer (20) filters for plasmid DNA precipitation, to be used with NucleoBond PC 100, PC 500, and Xtra Midi(EF) kits - 20 NucleoBond Finalizers, 2 syringes of 30 mL, and 2 syringes of 1 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740519.20: NucleoBond Finalizer
740511	NucleoBond® AX 20	20 Columns	USD $124.00
NucleoBond AX 20 Columns (20) columns for the isolation of plasmid DNA pack of 20 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740511: NucleoBond AX 20
740419.20	NucleoBond® Finalizer Large Plus	20 Finalizers	USD $107.00
NucleoBond Finalizer Large Plus (20 sets) filters for plasmid DNA precipitation, to be used with NucleoBond Xtra Maxi kits - 20 NucleoBond Finalizers Large, 20 syringes of 30 mL, and 20 syringes of 1 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740419.20: NucleoBond Finalizer Large Plus
740418.20	NucleoBond® Finalizer Large	20 Finalizers	USD $70.00
NucleoBond Finalizer Large (20) filters for plasmid DNA precipitation, to be used with NucleoBond Xtra Maxi kits - 20 NucleoBond Finalizers Large, 2 syringes of 30 mL, and 2 syringes of 1 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740418.20: NucleoBond Finalizer Large
740414.10S	NucleoBond® Xtra Maxi Columns	10 Columns	USD $203.00
Columns for NucleoBond Xtra Plasmid DNA Maxi preps. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Resources Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back 740414.10S: NucleoBond Xtra Maxi Columns Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740410.10S	NucleoBond® Xtra Midi Columns	10 Columns	USD $87.00
Columns for NucleoBond Xtra Midi plasmid DNA purification kit. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back 740410.10S: NucleoBond Xtra Midi Columns Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740414.100S	NucleoBond® Xtra Maxi Columns	100 Columns	USD $1405.00
Columns for NucleoBond Xtra Maxi plasmid DNA purification kit. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740414.100S: NucleoBond Xtra Maxi Columns

Overview

In accordance with Macherey-Nagel's Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.

More Information

Filters & finalizers
Product name	Quantity	Specification	Cat. #
NucleoBond Folded Filters	50	For use with NucleoBond AX 100 Columns	740561
NucleoBond Folded Filters XL	50	For use with NucleoBond AX 500 and AX 2000 Columns	740577
NucleoBond Bottle Top Filter Type 1	5	For use with NucleoBond AX 2000 cartridges	740547.5
NucleoBond Bottle Top Filter Type 2	5	For use with NucleoBond AX 10000 cartridges	740553.5
NucleoBond Finalizer	20	For use with NucleoBond Xtra Midi and Midi EF (20 Finalizers, 2 x 30 ml syringes & 2 x 1 ml syringes)	740519.20
NucleoBond Finalizer Plus	20	For use with NucleoBond Xtra Midi and Midi EF (20 Finalizers, 20 x 30 ml syringes & 20 x 1 ml syringes)	740520.20
NucleoBond Finalizer Large	20	For use with NucleoBond Xtra Maxi and Maxi EF (20 Finalizers, 2 x 30 ml syringes & 2 x 1 ml syringes)	740518.20
NucleoBond Finalizer Large Plus	20	For use with NucleoBond Xtra Maxi and Maxi EF (20 Finalizers, 20 x 30 ml syringes & 20 x 1 ml syringes)	740419.20

Columns
Product name	Quantity	Specification	Cat. #
NucleoBond Xtra Midi Columns	10	Columns with filters for Midi preps	740410.10S
NucleoBond Xtra Maxi Columns	10	Columns with filters for Maxi preps	740414.10S
NucleoBond Xtra Maxi Columns	100	Columns with filters for Maxi preps	740414.100S
NucleoBond AX 20	20	Anion exchange columns for purification of transfection-grade plasmid DNA—Mini preps	740511
NucleoBond AX 100	20	Anion exchange columns for purification of transfection-grade plasmid DNA—Midi preps	740521
NucleoBond AX 100	100	Anion exchange columns for purification of transfection-grade plasmid DNA—Midi preps	740521.100
NucleoBond AX 500	10	Anion exchange columns for purification of transfection-grade plasmid DNA—Maxi preps	740531
NucleoBond AX 500	50	Anion exchange columns for purification of transfection-grade plasmid DNA—Maxi preps	740531.50
NucleoBond AX 2000	10	Anion exchange columns for purification of transfection-grade plasmid DNA—Mega preps	740525
NucleoBond AX 10,000	5	Anion exchange columns for purification of transfection-grade plasmid DNA—Gigapreps	740534
NucleoBond AXG 20	20	Anion exchange columns for genomic DNA isolation from tissue and cells—Mini preps	740544
NucleoBond AXG 100	20	Anion exchange columns for genomic DNA isolation from tissue and cells—Midi preps	740545
NucleoBond AXG 500	10	Anion exchange columns for genomic DNA isolation from tissue and cells—Maxi preps	740546

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Isolate up to 700 μg of transfection-grade plasmid DNA in only 35 minutes

Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes

Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold.

Learn more Other plasmid kits

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

NucleoBond accessories & consumables

Notice to purchaser

740577: NucleoBond Folded Filters XL

Notice to purchaser

740561: NucleoBond Folded Filters

Notice to purchaser

NucleoBond Bottle Top Filter Type 2—for use with NucleoBond AX 10000 cartridges

740553.5: NucleoBond Bottle Top Filter Type 2

Notice to purchaser

NucleoBond Bottle Top Filter Type 1—for use with NucleoBond AX 2000 cartridges

740547.5: NucleoBond Bottle Top Filter Type 1

Notice to purchaser

740546: NucleoBond AXG 500

Notice to purchaser

Purification of genomic DNA from enterobacteria with NucleoBond AXG 100

740545: NucleoBond AXG 100

Notice to purchaser

740544: NucleoBond AXG 20

Notice to purchaser

740534: NucleoBond AX 10,000

Notice to purchaser

740531.50: NucleoBond AX 500

Notice to purchaser

740531: NucleoBond AX 500

Notice to purchaser

740525: NucleoBond AX 2000

Notice to purchaser

740521.100: NucleoBond AX 100

Notice to purchaser

740521: NucleoBond AX 100

Notice to purchaser

740520.20: NucleoBond Finalizer Plus

Notice to purchaser

740519.20: NucleoBond Finalizer

Notice to purchaser

740511: NucleoBond AX 20

Notice to purchaser

740419.20: NucleoBond Finalizer Large Plus

Notice to purchaser

740418.20: NucleoBond Finalizer Large

Notice to purchaser

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

740414.10S: NucleoBond Xtra Maxi Columns

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Notice to purchaser

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

740410.10S: NucleoBond Xtra Midi Columns

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Notice to purchaser

740414.100S: NucleoBond Xtra Maxi Columns

Overview

More Information

Additional product information