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Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Data showing the use of various kits to purify plasmids Data and information on plasmid purification kits
Home › Products › Nucleic acid purification › Accessories and components › Single-prep accessories & consumables › NucleoBond

Accessories and components

  • HTP accessories
    • NucleoMag Sep
    • NucleoVac 24 Vacuum Manifold
    • NucleoVac 96 Vacuum Manifold
    • Vacuum and robot accessories
  • Single-prep accessories & consumables
    • NucleoSpin
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  • Buffers & enzymes
    • NucleoType DirectLyse Reagent
    • NucleoSpin Plasmid
    • NucleoBond Plasmid
    • NucleoBond EF and RS
    • RNA isolation
    • NucleoSpin RNA/DNA Buffer Set
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    • Genomic DNA isolation
    • Enzymes and protein quantification
  • HTP consumables
    • 8-well strips
    • 96-well plates
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Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Data showing the use of various kits to purify plasmids Data and information on plasmid purification kits

NucleoBond accessories & consumables

Accessories for Nucleobond nucleic acid purification kits

Accessories and consumables for NucleoBond anion-exchange columns: folded filters and individual columns.

Cat. # Product Size Price License Quantity Details
740410.10S NucleoBond® Xtra Midi Columns 10 Columns USD $94.00

Columns for NucleoBond Xtra Midi plasmid DNA purification kit.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits
Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits.

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740410.10S: NucleoBond Xtra Midi Columns

740410.10S: NucleoBond Xtra Midi Columns

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Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume.

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NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

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Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).

740414.10S NucleoBond® Xtra Maxi Columns 10 Columns USD $220.00

Columns for NucleoBond Xtra Plasmid DNA Maxi preps.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

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NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

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740414.10S: NucleoBond Xtra Maxi Columns

740414.10S: NucleoBond Xtra Maxi Columns

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Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).

740418.20 NucleoBond® Finalizer Large 20 Finalizers USD $75.00

NucleoBond Finalizer Large (20) filters for plasmid DNA precipitation, to be used with NucleoBond Xtra Maxi kits - 20 NucleoBond Finalizers Large, 2 syringes of 30 mL, and 2 syringes of 1 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740418.20: NucleoBond Finalizer Large

740418.20: NucleoBond Finalizer Large
740419.20 NucleoBond® Finalizer Large Plus 20 Finalizers USD $117.00

NucleoBond Finalizer Large Plus (20 sets) filters for plasmid DNA precipitation, to be used with NucleoBond Xtra Maxi kits - 20 NucleoBond Finalizers Large, 20 syringes of 30 mL, and 20 syringes of 1 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740419.20: NucleoBond Finalizer Large Plus

740419.20: NucleoBond Finalizer Large Plus
740511 NucleoBond® AX 20 20 Columns USD $134.00

NucleoBond AX 20 Columns (20) columns for the isolation of plasmid DNA pack of 20

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740511: NucleoBond AX 20

740511: NucleoBond AX 20
740519.20 NucleoBond® Finalizer 20 Finalizers USD $52.00

NucleoBond Finalizer (20) filters for plasmid DNA precipitation, to be used with NucleoBond PC 100, PC 500, and Xtra Midi(EF) kits - 20 NucleoBond Finalizers, 2 syringes of 30 mL, and 2 syringes of 1 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740519.20: NucleoBond Finalizer

740519.20: NucleoBond Finalizer
740520.20 NucleoBond® Finalizer Plus 20 Finalizers USD $94.00

NucleoBond Finalizer Plus (20) filters for plasmid DNA precipitation, to be used with NucleoBond PC 100, PC 500, and Xtra Midi(EF) kits - 20 NucleoBond Finalizers, 20 syringes of 30 mL, and 20 syringes of 1 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740520.20: NucleoBond Finalizer Plus

740520.20: NucleoBond Finalizer Plus
740521 NucleoBond® AX 100 20 Columns USD $208.00

NucleoBond AX 100 Columns (20) columns for the isolation of plasmid DNA pack of 20

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740521: NucleoBond AX 100

740521: NucleoBond AX 100
740521.100 NucleoBond® AX 100 100 Columns USD $885.00

NucleoBond AX 100 Columns (100) columns for the isolation of plasmid DNA pack of 100

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740521.100: NucleoBond AX 100

740521.100: NucleoBond AX 100
740525 NucleoBond® AX 2000 10 Columns USD $418.00

NucleoBond AX 2000 Columns (10) columns for the isolation of plasmid DNA pack of 10

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740525: NucleoBond AX 2000

740525: NucleoBond AX 2000
740531 NucleoBond® AX 500 10 Columns USD $200.00

NucleoBond AX 500 Columns (10) columns for the isolation of plasmid DNA pack of 10

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740531: NucleoBond AX 500

740531: NucleoBond AX 500
740531.50 NucleoBond® AX 500 50 Columns USD $965.00

NucleoBond AX 500 Columns (50) columns for the isolation of plasmid DNA pack of 50

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740531.50: NucleoBond AX 500

740531.50: NucleoBond AX 500
740534 NucleoBond® AX 10,000 5 Columns USD $441.00

NucleoBond AX 10000 Columns (5) columns for the isolation of plasmid DNA pack of 5

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740534: NucleoBond AX 10,000

740534: NucleoBond AX 10,000
740544 NucleoBond® AXG 20 20 Columns USD $164.00

NucleoBond AXG 20 Columns (20) columns (no buffers provided) for the isolation of genomic DNA from cells, tissue, and bacteria. Combine NucleoBond AXG columns and the corresponding buffer set (III or IV) for highest flexibility.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740544: NucleoBond AXG 20

740544: NucleoBond AXG 20
740545 NucleoBond® AXG 100 20 Columns USD $232.00

NucleoBond AXG 100 Columns (20) columns (no buffers provided) for the isolation of genomic DNA from cells, tissue, and bacteria. Combine NucleoBond AXG columns and the corresponding buffer set (III or IV) for highest flexibility.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Purification of genomic DNA from enterobacteria with NucleoBond AXG 100

Purification of genomic DNA from enterobacteria with NucleoBond AXG 100
Purification of genomic DNA from enterobacteria with NucleoBond AXG 100. Lane 1. Molecular weight marker. Lane 2. Purified genomic DNA.

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740545: NucleoBond AXG 100

740545: NucleoBond AXG 100
740546 NucleoBond® AXG 500 10 Columns USD $260.00

NucleoBond AXG 500 Columns (10) columns (no buffers provided) for the isolation of genomic DNA from cells, tissue, and bacteria. Combine NucleoBond AXG columns and the corresponding buffer set (III or IV) for highest flexibility.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740546: NucleoBond AXG 500

740546: NucleoBond AXG 500
740547.5 NucleoBond® Bottle Top Filter Type 1 5 Filters USD $99.00

NucleoBond Bottle Top Filter Type 1 (5) NucleoBond Bottle Top Filters for use with NucleoBond AX 2000 Columns pack of 5

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond Bottle Top Filter Type 1—for use with NucleoBond AX 2000 cartridges

NucleoBond Bottle Top Filter Type 1—for use with NucleoBond AX 2000 cartridges

NucleoBond Bottle Top Filter Type 1—for use with NucleoBond AX 2000 cartridges.

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740547.5: NucleoBond Bottle Top Filter Type 1

740547.5: NucleoBond Bottle Top Filter Type 1
740553.5 NucleoBond® Bottle Top Filter Type 2 5 Filters USD $115.00

NucleoBond Bottle Top Filter Type 2 (5) 5 NucleoBond Bottle Top Filters for use with NucleoBond AX 10000 Columns pack of 5

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoBond Bottle Top Filter Type 2—for use with NucleoBond AX 10000 cartridges

NucleoBond Bottle Top Filter Type 2—for use with NucleoBond AX 10000 cartridges

NucleoBond Bottle Top Filter Type 2—for use with NucleoBond AX 10000 cartridges.

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740553.5: NucleoBond Bottle Top Filter Type 2

740553.5: NucleoBond Bottle Top Filter Type 2
740561 NucleoBond® Folded Filters 50 Filters USD $50.00

NucleoBond Folded Filters (50) folded filters for NucleoBond AX 100 Columns pack of 50

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740561: NucleoBond Folded Filters

740561: NucleoBond Folded Filters
740577 NucleoBond® Folded Filters XL 50 Filters USD $61.00

NucleoBond Folded Filters XL (50) folded filters for NucleoBond AX 500 and AX 2000 Columns pack of 50

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740577: NucleoBond Folded Filters XL

740577: NucleoBond Folded Filters XL
740414.100S NucleoBond® Xtra Maxi Columns 100 Columns USD $1520.00

Columns for NucleoBond Xtra Maxi plasmid DNA purification kit.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740414.100S: NucleoBond Xtra Maxi Columns

740414.100S: NucleoBond Xtra Maxi Columns

NucleoBond racks Racks compatible with NucleoBond columns
Technical notes Data: plasmid preps for transfection
Purification kits for isolating DNA from cultured cells, blood, tissue, plants and hard to lyse samples such as stool and soil Genomic DNA purification kits

Overview

In accordance with Macherey-Nagel's Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.

More Information

Filters & finalizers
Product name Quantity Specification Cat. #
NucleoBond Folded Filters 50 For use with NucleoBond AX 100 Columns 740561
NucleoBond Folded Filters XL 50 For use with NucleoBond AX 500 and AX 2000 Columns 740577
NucleoBond Bottle Top Filter Type 1 5 For use with NucleoBond AX 2000 cartridges 740547.5
NucleoBond Bottle Top Filter Type 2 5 For use with NucleoBond AX 10000 cartridges 740553.5
NucleoBond Finalizer 20 For use with NucleoBond Xtra Midi and Midi EF (20 Finalizers, 2 x 30 ml syringes & 2 x 1 ml syringes) 740519.20
NucleoBond Finalizer Plus 20 For use with NucleoBond Xtra Midi and Midi EF (20 Finalizers, 20 x 30 ml syringes & 20 x 1 ml syringes) 740520.20
NucleoBond Finalizer Large 20 For use with NucleoBond Xtra Maxi and Maxi EF (20 Finalizers, 2 x 30 ml syringes & 2 x 1 ml syringes) 740518.20
NucleoBond Finalizer Large Plus 20 For use with NucleoBond Xtra Maxi and Maxi EF (20 Finalizers, 20 x 30 ml syringes & 20 x 1 ml syringes) 740419.20

Columns
Product name Quantity Specification Cat. #
NucleoBond Xtra Midi Columns 10 Columns with filters for Midi preps 740410.10S
NucleoBond Xtra Maxi Columns 10 Columns with filters for Maxi preps 740414.10S
NucleoBond Xtra Maxi Columns 100 Columns with filters for Maxi preps 740414.100S
NucleoBond AX 20 20 Anion exchange columns for purification of transfection-grade plasmid DNA—Mini preps 740511
NucleoBond AX 100 20 Anion exchange columns for purification of transfection-grade plasmid DNA—Midi preps 740521
NucleoBond AX 100 100 Anion exchange columns for purification of transfection-grade plasmid DNA—Midi preps 740521.100
NucleoBond AX 500 10 Anion exchange columns for purification of transfection-grade plasmid DNA—Maxi preps 740531
NucleoBond AX 500 50 Anion exchange columns for purification of transfection-grade plasmid DNA—Maxi preps 740531.50
NucleoBond AX 2000 10 Anion exchange columns for purification of transfection-grade plasmid DNA—Mega preps 740525
NucleoBond AX 10,000 5 Anion exchange columns for purification of transfection-grade plasmid DNA—Gigapreps 740534
NucleoBond AXG 20 20 Anion exchange columns for genomic DNA isolation from tissue and cells—Mini preps 740544
NucleoBond AXG 100 20 Anion exchange columns for genomic DNA isolation from tissue and cells—Midi preps 740545
NucleoBond AXG 500 10 Anion exchange columns for genomic DNA isolation from tissue and cells—Maxi preps 740546

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Isolate up to 700 μg of transfection-grade plasmid DNA in only 35 minutes

Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes

Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold. 

Learn more Other plasmid kits

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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