Plasmid DNA purification

NucleoSpin buffers for plasmid isolation with silica membrane miniprep spin columns.

Choose buffers for silica membrane miniprep spin columns when isolating plasmids using NucleoSpin Plasmid kits.

Cat. #	Product	Size	Price
740953	NucleoSpin® Plasmid Buffer Set	Each	USD $53.00
NucleoSpin Plasmid Buffer Set for the isolation of low-copy plasmids, only applicable with NucleoSpin Plasmid kits, Buffers A1, A2, A3, RNase A sufficient for 300 preps Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740953: NucleoSpin Plasmid Buffer Set
740917.1	Buffer AE	1,000 mL	USD $85.00
Buffer AE (1000 mL) Elution Buffer AE bottle of 1000 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740917.1: Buffer AE
740916.1	Buffer AW	1,000 mL	USD $170.00
Buffer AW (1000 mL) Wash Buffer AW bottle of 1000 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740916.1: Buffer AW
740914.1	Buffer A4 Concentrate	200 mL	USD $106.00
Buffer A4 Concentrate (200 mL) Wash Buffer A4 Concentrate, for 1000 mL Buffer A4 bottle of 200 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740914.1: Buffer A4 Concentrate
740914	Buffer A4 Concentrate	20 mL	USD $36.00
Buffer A4 Concentrate (25 mL) Wash Buffer A4 Concentrate, for 125 mL Buffer A4 bottle of 25 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740914: Buffer A4 Concentrate
740913.1	Buffer A3	1,000 mL	USD $170.00
Buffer A3 (1000 mL) Neutralization Buffer A3 bottle of 1000 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740913.1: Buffer A3
740911.1	Buffer A1, without RNase A	1,000 mL	USD $91.00
Buffer A1 (1000 mL) Resuspension Buffer A1, without RNase A bottle of 1000 mL Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740911.1: Buffer A1, without RNase A
740995	Buffer AQ Concentrate	25 mL	USD $41.00
Buffer AQ Concentrate is a wash buffer provided with NucleoSpin Plasmid QuickPure kits for rapid plasmid purification. Cat. # 740995 consists of a bottle of 25 ml of concentrated Buffer AQ, which should be diluted to a working solution of 125 ml prior to use. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740995: Buffer AQ Concentrate

More Information

In accordance with Macherey-Nagel´s Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.

Product name	Size	Specification	Cat. #
NucleoSpin Plasmid Buffer Set	1 set	For isolation of low-copy plasmids Only applicable to NucleoSpin Plasmid kits. Set includes Buffers A1, A2, A3, and RNase A. Suitable for 300 minipreps	740953
Buffer A1, without RNase A	1,000 ml	Resuspension buffer, without RNase A	740911.1
Buffer A2	1,000 ml	Lysis buffer	740912.1
Buffer A3	1,000 ml	Neutralization buffer	740913.1
Buffer AQ Concentrate	25 ml	Wash buffer concentrate (for 125 ml of Buffer AQ)	740995
Buffer A4 Concentrate	20 ml	Wash buffer concentrate (for 100 ml of Buffer A4)	740914
Buffer A4 Concentrate	200 ml	Wash buffer concentrate (for 1,000 ml of Buffer A4)	740914.1
Buffer AE	1,000 ml	Elution buffer	740917.1
Buffer AW	1,000 ml	Wash buffer	740916.1

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Tip for a successful transfection—remove endotoxins!

Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.

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Plasmid DNA purification

Notice to purchaser

740953: NucleoSpin Plasmid Buffer Set

Notice to purchaser

740917.1: Buffer AE

Notice to purchaser

740916.1: Buffer AW

Notice to purchaser

740914.1: Buffer A4 Concentrate

Notice to purchaser

740914: Buffer A4 Concentrate

Notice to purchaser

740913.1: Buffer A3

Notice to purchaser

740911.1: Buffer A1, without RNase A

Notice to purchaser

740995: Buffer AQ Concentrate

More Information

Additional product information