Plasmid DNA purification
Choose buffers for silica membrane miniprep spin columns when isolating plasmids using NucleoSpin Plasmid kits.
More Information
In accordance with Macherey-Nagel´s Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.
| Product name | Size | Specification | Cat. # |
| NucleoSpin Plasmid Buffer Set |
1 set |
For isolation of low-copy plasmids |
740953 |
| Buffer A1, without RNase A |
1,000 ml | Resuspension buffer, without RNase A | 740911.1 |
| Buffer A2 | 1,000 ml | Lysis buffer | 740912.1 |
| Buffer A3 | 1,000 ml | Neutralization buffer | 740913.1 |
| Buffer AQ Concentrate | 25 ml | Wash buffer concentrate (for 125 ml of Buffer AQ) | 740995 |
| Buffer A4 Concentrate | 20 ml | Wash buffer concentrate (for 100 ml of Buffer A4) | 740914 |
| Buffer A4 Concentrate | 200 ml | Wash buffer concentrate (for 1,000 ml of Buffer A4) | 740914.1 |
| Buffer AE | 1,000 ml | Elution buffer | 740917.1 |
| Buffer AW | 1,000 ml | Wash buffer | 740916.1 |
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tip for a successful transfection—remove endotoxins!
Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.
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