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RNA isolation—NucleoSpin buffers

Buffers and reagents for RNA isolation using NucleoSpin silica membrane technology.

Cat. # Product Size License Quantity Details
740963 rDNase Set Each USD $84.00 *

The rDNase Set is intended for performing RNA minipreps using NucleoSpin RNA kits. Cat. # 740963 consists of one vial of RNase-free recombinant DNase (lyophilized), plus 7 ml Reaction Buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740961.500 Buffer RA1 500 mL USD $172.00 *

Buffer RA1 is a lysis buffer included with NucleoSpin RNA and NucleoSpin RNA XS kits. Cat. # 740961.500 consists of a 500-ml bottle of Buffer RA1.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740961 Buffer RA1 50 mL USD $35.00 *

Buffer RA1 is a lysis buffer included with NucleoSpin RNA and NucleoSpin RNA XS kits. Cat. # 740961 consists of a 60-ml bottle of Buffer RA1.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740960 Buffer RA4 Concentrate 300 mL USD $92.00 *

Buffer RA4 Concentrate consists of a 300-ml bottle of concentrate that makes 1000 ml of Buffer RA4.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740941 Protein Solving Buffer Set PSB/TCEP Each USD $118.00 *

Protein Solving Buffer Set PSB/TCEP can be used with NucleoSpin RNA/Protein and NucleoSpin TriPrep kits. Cat. # 740941 consists of one 7.5 ml bottle of Buffer PSB and 107 mg of the reducing agent TCEP.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740936.500 Buffer RAP 500 mL USD $179.00 *

NucleoSpin RNA lysis buffer for extraction of total RNA from plant samples with silica membrane miniprep spin columns. Contains guanidinium-HCl. Cat. # 740936.500 consists of 500 ml of Buffer RAP.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740936.50 Buffer RAP 50 mL USD $36.00 *

NucleoSpin RNA lysis buffer for extraction of total RNA from plant samples with silica membrane miniprep spin columns. Contains guanidinium-HCl. Cat. # 740936.50 consists of 50 ml of Buffer RAP.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740934.500 Buffer RP1 500 mL USD $174.00 *

Buffer RP1 is a NucleoSpin RNA lysis buffer for total RNA extraction with silica membrane miniprep spin columns. Cat. # 740934.500 consists of a 500-ml bottle of Buffer RP1.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740934.50 Buffer RP1 50 mL USD $32.00 *

Buffer RP1 is a NucleoSpin RNA lysis buffer for total RNA extraction with silica membrane miniprep spin columns. Cat. # 740934.50 consists of a 50-ml bottle of Buffer RP1.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740385.125 Buffer RL1 125 mL USD $63.00 *

Buffer RL1 is a lysis buffer for extraction of total RNA from plant samples with a high detergent concentration. Cat. # 740385.125 consists of a 125-ml bottle of Lysis Buffer RL1.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740360.25 Buffer RA3 Concentrate 25 mL USD $28.00 *

NucleoSpin RNA lysis buffers for total RNA extraction with silica membrane miniprep spin columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740367.25 Buffer MPP 25 mL USD $45.00 *

Buffer MPP is a protein precipitation buffer included and used with the NucleoSpin miRNA Plasma kit.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740202.32 Buffer DL 100 mL USD $36.00 *

Buffer DL is a lysis buffer included with NucleoSpin kits for RNA isolation from blood. Cat. # 740202.32 consists of a bottle containing 100 ml of Buffer DL.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740364.80 Buffer RAW2 80 mL USD $42.00 *

Buffer RAW2 is a wash buffer included with NucleoSpin RNA kits. Cat. # 740364.80 consists of a bottle containing 80 ml of Buffer RAW2.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740994.100 Buffer MW2 Concentrate 100 mL USD $62.00 *

Buffer MW2 Concentrate is a wash buffer used for RNA purification that is included in NucleoSpin miRNA kits. Cat. # 740994.100 consists of a bottle of 100 ml of concentrated Buffer MW2, which should be diluted to a working solution of 500 ml prior to use.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740834.60 Reaction Buffer for rDNase 60 mL USD $32.00 *

Reaction Buffer for rDNase is provided with many RNA purification kits as a reaction buffer for DNase-mediated DNA digestion. Cat. # 740834.60 consists of 60 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740906.125 Buffer LBP 125 mL USD $95.00 *

Buffer LBP is a lysis buffer included with NucleoSpin RNA Plus kits for purification of total RNA. Cat. # 740906.125 consists of a bottle containing 125 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740368.30 Buffer LB1 30 mL USD $40.00 *

Buffer LB1 is a lysis buffer included with NucleoSpin RNA Plus XS kits for purification of total RNA from extra small samples. Cat. # 740368.30 consists of a bottle containing 30 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740369.30 Buffer LB2 30 mL USD $40.00 *

Buffer LB2 is a lysis buffer included with NucleoSpin RNA Plus XS kits for purification of total RNA from extra small samples. Cat. # 740369.30 consists of a bottle containing 30 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740514 Carrier RNA (Lyophilized) 300 ug USD $35.00 *

Carrier RNA (Lyophilized) can be used to increase the yield of RNA purified from low inputs. It can be used with the NucleoSpin® RNA XS, RNA Virus kits, or NucleoMag® Pathogen kit.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740359.80 Buffer RA2 80 mL USD $42.00 *

Buffer RA2 is a wash buffer included with NucleoSpin RNA, NucleoSpin RNA Midi, NucleoSpin RNA XS, NucleoSpin 8/96 RNA, and NucleoSpin RNA Plant kits. Cat. # 740359.80 consists of a 80-ml bottle of Buffer RA2.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740361.150 Buffer RAW 150 mL USD $59.00 *

Buffer RAW is a wash buffer included with NucleoSpin RNA Virus kits. Cat. # 740361.150 consists of a 150-ml bottle of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740365.75 Buffer MLP 75 mL USD $57.00 *

Buffer MLP is a lysis buffer included with NucleoSpin miRNA Plasma kits. Cat. # 740365.75 consists of a 75-ml bottle of Buffer MLP.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740342.125 Buffer MDB 125 mL USD $36.00 *

Buffer MDB is a membrane-desalting buffer included with NucleoSpin RNA, NucleoSpin RNA XS, NucleoSpin RNA Blood, and NucleoSpin miRNA kits. Cat. # 740342.125 consists of a 125-ml bottle of Buffer MDB.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740405.60 Buffer MX 60 mL USD $98.00 *

Buffer MX is a binding buffer included with NucleoSpin miRNA and NucleoSpin totalRNA FFPE kits. Cat. # 740405.60 consists of a 60-ml bottle of Buffer MX.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740907.30 Binding Solution BS 30 mL USD $31.00 *

Binding Solution BS is a binding buffer included with NucleoSpin RNA Plus kits. Cat. # 740907.30 consists of a 30-ml bottle of Binding Solution BS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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744872 Carrier RNA Buffer 2 mL USD $26.10 *

Carrier RNA Buffer can be used to solubilize Carrier RNA (Lyophilized), cat. # 740514. The resulting solution can be used to increase the yield of RNA purified from low inputs. It can be used with the NucleoSpin® RNA XS, RNA Virus kits, or NucleoMag® Pathogen kit.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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More Information

In accordance with Macherey-Nagel's Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.

Product name Size Specification Cat. #
Buffer DL 100 ml Lysis buffer included with NucleoSpin kits for RNA isolation from blood 740202.32
Buffer MLP 75 ml Lysis buffer included with NucleoSpin miRNA Plasma kits 740365.75
Buffer MPP 25 ml Protein precipitation buffer included with NucleoSpin miRNA Plasma kits 740367.25
Buffer RA1 50 ml Lysis buffer included with NucleoSpin RNA and NucleoSpin RNA XS kits 740961
500 ml 740961.500
Buffer LBP 125 ml Lysis buffer included with NucleoSpin RNA Plus kits 740906.125
Buffer LB1 30 ml Lysis buffers included with NucleoSpin RNA Plus XS kits 740368.30
Buffer LB2 30 ml 740369.30
Binding Solution BS 30 ml Binding buffer included with NucleoSpin RNA Plus kits 740907.30
Buffer RA2 80 ml Wash buffers included with NucleoSpin RNA kits 740359.80
Buffer RA3 Concentrate 25 ml 740360.25
Buffer RA4 Concentrate 300 ml 740960
Buffer RAP 50 ml Lysis buffer for extraction of total RNA from plant samples—contains guanidinium-HCl 740936.50
500 ml 740936.500
Buffer RL1 50 ml Lysis buffer for extraction of total RNA from plant samples—has a high detergent concentration 740385.50
125 ml 740385.125
Buffer RP1 50 ml Lysis buffer 740934.50
500 ml 740934.500
Buffer MX 60 ml Binding buffer included with NucleoSpin miRNA and NucleoSpin totalRNA FFPE kits 740405.60
Buffer RAW 150 ml Wash buffer included with NucleoSpin RNA Virus kits 740361.150
Buffer RAW2 80 ml

Wash buffer included with NucleoSpin RNA kits

740364.80
Buffer MW2 Concentrate 100 ml

Wash buffer concentrate (for 500 ml of Buffer MW2)

740944.100
Protein Solving Buffer Set PSB/TCEP 1 set

7.5 ml Buffer PSB and 107 mg TCEP (reducing agent). Only applicable to NucleoSpin RNA/Protein kits.

740941
rDNase Set 1 set Recombinant DNase and Reaction Buffer for rDNase. Sufficient for 50 total RNA minipreps. 740963
Reaction Buffer for rDNase 60 ml Reaction buffer for rDNase (included with many RNA purification kits) 740834.60
Carrier RNA (Lyophilized) 0.3 mg Lyophilized carrier RNA to be used with NucleoSpin RNA kits 740514
Carrier RNA Buffer 2 ml Buffer used to solubilize Carrier RNA (Lyophilized) 744872
Buffer MDB 125 ml Membrane-desalting buffer included with NucleoSpin RNA kits 740342.125

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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