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  • ‹ Back to Buffers & enzymes
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Technical notes and data highlighting the performance of our nucleic acid purification kits Learning center

DNA cleanup accessories

Extra buffers sold separately for NucleoTrap and NucleoSpin Gel and PCR Kits

Choose additional buffers, sold separately, for use with NucleoTrap and NucleoSpin Gel and PCR Clean-Up kits for isolation of highly pure DNA fragments from agarose gels and aqueous solutions.

Cat. # Product Size Price License Quantity Details
740614.100 Buffer NT 100 mL USD $43.00

Buffer NT (75 mL) Binding Buffer NT bottle of 75 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740614.100: Buffer NT

740614.100: Buffer NT
740614.1 Buffer NT 1,000 mL USD $165.00

NucleoSpin Extract II & NucleoTrap Buffers for DNA clean-up spin columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740614.1: Buffer NT

740614.1: Buffer NT
740596.100 Buffer NT1 100 mL USD $34.00

Buffer NT1 (100 mL) Binding Buffer NT1 bottle of 100 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740596.100: Buffer NT1

740596.100: Buffer NT1
740597 Buffer NT2 100 mL USD $35.00

Buffer NT2 (100 mL) Binding Buffer NT2 1 bottle of 100 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740597: Buffer NT2

740597: Buffer NT2
740598 Buffer NT3 Concentrate 25 mL USD $35.00

Buffer NT3 Concentrate (25 mL) Wash Buffer NT3 Concentrate, for 125 mL Buffer NT3 bottle of 25 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740598: Buffer NT3 Concentrate

740598: Buffer NT3 Concentrate
740595.150 Buffer NTB 150 mL USD $32.00

For clean-up of SDS-containing samples when using NucleoSpin Gel and PCR kits. Includes binding Buffer NTB, 1 bottle of 150 mL.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740595.150: Buffer NTB

740595.150: Buffer NTB

Required Products

Cat. # Product Size Price License Quantity Details
740609.250 NucleoSpin® Gel and PCR Clean-Up 250 Preps USD $414.00

NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers

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Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes
Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes.

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The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup  kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

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740609.250: NucleoSpin Gel and PCR Clean-Up

740609.250: NucleoSpin Gel and PCR Clean-Up
740595.1 Buffer NTB 1,000 mL USD $142.00

For clean-up of SDS-containing samples when using NucleoSpin Gel and PCR kits. Includes binding Buffer NTB, 1 bottle of 1000 mL.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740595.1: Buffer NTB

740595.1: Buffer NTB

Required Products

Cat. # Product Size Price License Quantity Details
740609.250 NucleoSpin® Gel and PCR Clean-Up 250 Preps USD $414.00

NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers

Documents You May Also Like Image Data

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Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes
Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes.

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The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup  kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

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740609.250: NucleoSpin Gel and PCR Clean-Up

740609.250: NucleoSpin Gel and PCR Clean-Up
740654.100 Buffer NTC 125 mL USD $38.00

For clean-up of single stranded DNA when combined with NucleoSpin Gel and PCR Clean-up Kits. Includes 1 bottle of 125 mL.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740654.100: Buffer NTC

740654.100: Buffer NTC

Required Products

Cat. # Product Size Price License Quantity Details
740609.250 NucleoSpin® Gel and PCR Clean-Up 250 Preps USD $414.00

NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers

Documents You May Also Like Image Data

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Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes
Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes.

Back

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup  kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

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740609.250: NucleoSpin Gel and PCR Clean-Up

740609.250: NucleoSpin Gel and PCR Clean-Up
740305.120 Buffer NTI 120 mL USD $53.00

Buffer NTI is a binding buffer developed for use with NucleoSpin Gel and PCR Clean-up kits. Included in the buffer is a pH indicator that enables users to verify whether conditions on the NucleoSpin column are optimal for binding DNA purified from agarose gels or PCR reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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740305.120: Buffer NTI

740305.120: Buffer NTI

Required Products

Cat. # Product Size Price License Quantity Details
740609.250 NucleoSpin® Gel and PCR Clean-Up 250 Preps USD $414.00

NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers

Documents You May Also Like Image Data

Back

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes
Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes.

Back

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup  kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

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740609.250: NucleoSpin Gel and PCR Clean-Up

740609.250: NucleoSpin Gel and PCR Clean-Up
740609.50 NucleoSpin® Gel and PCR Clean-Up 50 Preps USD $90.00

NucleoSpin Gel and PCR Clean-up (50) 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers

Documents You May Also Like Image Data

Back

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes
Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes.

Back

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup  kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

Back

740609.50: NucleoSpin Gel and PCR Clean-Up

740609.50: NucleoSpin Gel and PCR Clean-Up
740609.240C NucleoSpin® Gel and PCR Clean-Up 240 Preps USD $414.00

250 prep clean-up Kit for use on QIAcube includes NucleoSpin® Gel and PCR Clean-up Columns, buffers.

Documents You May Also Like Image Data

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740609.240C: NucleoSpin Gel and PCR Clean-Up

740609.240C: NucleoSpin Gel and PCR Clean-Up
740609.12C NucleoSpin® Gel and PCR Clean-Up (Sample-Not for Sale) 12 Preps USD $79.00

NucleoSpin® Gel and PCR Clean-Up (Sample-Not for Sale)

Documents Image Data

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740609.12C: NucleoSpin Gel and PCR Clean-Up (Sample-Not for Sale)

740609.12C: NucleoSpin Gel and PCR Clean-Up (Sample-Not for Sale)
740609.10 NucleoSpin® Gel and PCR Clean-Up Kit (Sample-Not for Sale) 10 Each USD $25.10

NucleoSpin® Gel and PCR Clean-Up Kit (Sample-Not for Sale)

Documents Image Data

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740609.10: NucleoSpin Gel and PCR Clean-Up Kit (Sample-Not for Sale)

740609.10: NucleoSpin Gel and PCR Clean-Up Kit (Sample-Not for Sale)

DNA cleanup kits product page section Gel and PCR cleanup kits
Data showing the use of various kits to extract DNA frm agarose, purify PCR products or clean-up RNA preps Cleanup and extraction kits
Purification of PCR fragments in an 8-well strip format NucleoSpin 8-well PCR cleanup kit

More Information

In accordance with Macherey Nagel's Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.

Product name Size Specification Cat. #
Buffer NT 100 ml
1,000 ml
Binding buffer 740614.100
740614.1
Buffer NTI 120 ml Binding buffer with pH indicator 740305.120
Buffer NT1 100 ml Binding buffer 740596.100
Buffer NT2 100 ml Binding/wash buffer 740597
Buffer NT3 Concentrate 20 ml Wash buffer concentrate
(for 100 ml of Buffer NT3)
740598
Buffer NTB 150 ml
1,000 ml
Binding buffer
For clean up of samples that contain SDS
740595.150
740595.1
Buffer NTC 100 ml Binding buffer
For clean up of single-stranded DNA
740654.100

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


DNA cleanup: two applications in one kit

The NucleoSpin Gel and PCR Clean-Up procedure is the easiest way to purify DNA fragments from agarose gels or PCR reactions. The kit includes one buffer for both applications, which contains a pH indicator that displays the correct pH for optimal kit performance. The purification procedure for samples from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

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