DNA cleanup accessories

Buffer NTI is a binding buffer developed for use with NucleoSpin Gel and PCR Clean-up kits. Included in the buffer is a pH indicator that enables users to verify whether conditions on the NucleoSpin column are optimal for binding DNA purified from agarose gels or PCR reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. #	Product	Size	Price
740609.250	NucleoSpin® Gel and PCR Clean-Up	250 Preps	USD $505.00
NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers Documents You May Also Like Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back 740609.250: NucleoSpin Gel and PCR Clean-Up Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).
740609.50	NucleoSpin® Gel and PCR Clean-Up	50 Preps	USD $111.00
NucleoSpin Gel and PCR Clean-up (50) 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers Documents You May Also Like Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5). Back 740609.50: NucleoSpin Gel and PCR Clean-Up
740609.240C	NucleoSpin® Gel and PCR Clean-Up	240 Preps	USD $505.00
250 prep clean-up Kit for use on QIAcube includes NucleoSpin® Gel and PCR Clean-up Columns, buffers. Documents You May Also Like Image Data Back 740609.240C: NucleoSpin Gel and PCR Clean-Up
740609.10	NucleoSpin® Gel and PCR Clean-Up Kit (Sample-Not for Sale)	10 Each	USD $31.00
NucleoSpin® Gel and PCR Clean-Up Kit (Sample-Not for Sale) Documents Image Data Back 740609.10: NucleoSpin Gel and PCR Clean-Up Kit (Sample-Not for Sale)
740609.12C	NucleoSpin® Gel and PCR Clean-Up (Sample-Not for Sale)	12 Preps	USD $94.00
NucleoSpin® Gel and PCR Clean-Up (Sample-Not for Sale) Documents Image Data Back 740609.12C: NucleoSpin Gel and PCR Clean-Up (Sample-Not for Sale)

For clean-up of single stranded DNA when combined with NucleoSpin Gel and PCR Clean-up Kits. Includes 1 bottle of 125 mL.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740609.250	NucleoSpin® Gel and PCR Clean-Up	250 Preps	USD $505.00
NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers Documents You May Also Like Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back 740609.250: NucleoSpin Gel and PCR Clean-Up Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

Buffer NT (75 mL) Binding Buffer NT bottle of 75 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

For clean-up of SDS-containing samples when using NucleoSpin Gel and PCR kits. Includes binding Buffer NTB, 1 bottle of 1000 mL.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740609.250	NucleoSpin® Gel and PCR Clean-Up	250 Preps	USD $505.00
NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers Documents You May Also Like Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back 740609.250: NucleoSpin Gel and PCR Clean-Up Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

For clean-up of SDS-containing samples when using NucleoSpin Gel and PCR kits. Includes binding Buffer NTB, 1 bottle of 150 mL.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740609.250	NucleoSpin® Gel and PCR Clean-Up	250 Preps	USD $505.00
NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers Documents You May Also Like Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back 740609.250: NucleoSpin Gel and PCR Clean-Up Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

Buffer NT1 (100 mL) Binding Buffer NT1 bottle of 100 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Buffer NT2 (100 mL) Binding Buffer NT2 1 bottle of 100 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Buffer NT3 Concentrate (25 mL) Wash Buffer NT3 Concentrate, for 125 mL Buffer NT3 bottle of 25 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Product name	Size	Specification	Cat. #
Buffer NT	100 ml 1,000 ml	Binding buffer	740614.100 740614.1
Buffer NTI	120 ml	Binding buffer with pH indicator	740305.120
Buffer NT1	100 ml	Binding buffer	740596.100
Buffer NT2	100 ml	Binding/wash buffer	740597
Buffer NT3 Concentrate	20 ml	Wash buffer concentrate (for 100 ml of Buffer NT3)	740598
Buffer NTB	150 ml 1,000 ml	Binding buffer For clean up of samples that contain SDS	740595.150 740595.1
Buffer NTC	100 ml	Binding buffer For clean up of single-stranded DNA	740654.100

Accessories and components

DNA cleanup accessories

Notice to purchaser

Notice to purchaser

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More Information

Additional product information

DNA cleanup accessories

Notice to purchaser

740305.120: Buffer NTI

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

740609.250: NucleoSpin Gel and PCR Clean-Up

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

740609.50: NucleoSpin Gel and PCR Clean-Up

740609.240C: NucleoSpin Gel and PCR Clean-Up

740609.10: NucleoSpin Gel and PCR Clean-Up Kit (Sample-Not for Sale)

740609.12C: NucleoSpin Gel and PCR Clean-Up (Sample-Not for Sale)

Notice to purchaser

740654.100: Buffer NTC

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

740609.250: NucleoSpin Gel and PCR Clean-Up

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

Notice to purchaser

740614.100: Buffer NT

Notice to purchaser

740595.1: Buffer NTB

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

740609.250: NucleoSpin Gel and PCR Clean-Up

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

Notice to purchaser

740595.150: Buffer NTB

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

740609.250: NucleoSpin Gel and PCR Clean-Up

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

Notice to purchaser

740596.100: Buffer NT1

Notice to purchaser

740597: Buffer NT2

Notice to purchaser

740598: Buffer NT3 Concentrate

More Information

Additional product information