PCR cleanup and gel extraction kits

Purify DNA fragments from agarose gels or PCR reactions with a kit containing a buffer with a pH indicator for optimal performance.

With the NucleoSpin Gel and PCR Clean-Up kits, you get two applications in one. The supplied columns, which are suitable for gel extraction as well as PCR purification, bind DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded onto the column, contaminants are removed by simple wash steps with ethanolic Wash Buffer NT3—providing rapid DNA purification. You can isolate fragments from 50 bp–20 kb in minimal elution volumes (as low as 15 µl for the mini kit, 200 µl for the midi kit, and 1 ml for the maxi kit) with recovery rates of 70–95%. Downstream applications include sequencing, ligation, labeling, cloning, and restriction enzyme digestion.

The NucleoSpin Gel and PCR Clean-Up kit was formerly known as the NucleoSpin Extract II kit.

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Cat. #	Product	Size	Price
740986.20	NucleoSpin® Gel & PCR Clean-up Midi	20 Preps	$202.00
The NucleoSpin Gel & PCR Clean-up Midi kit includes spin columns, collection tubes, and buffers for purification of DNA from PCR reactions or agarose gels. The kit enables recovery of DNA fragments ranging in size from 50 bp–20 kb, and can be used to process up to 4 ml of PCR reaction mixture or 4 g of agarose gel in one prep. The binding capacity of the midi spin column is 75 µg, and typical elution volumes range from 200–400 µl. The kit employs a user-friendly workflow that enables processing of 6 samples in ~25 minutes. Cat. # 740986.20 includes sufficient quantities of reagents and materials for performing 20 preps. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Complete primer removal down to 65 bp using NucleoSpin Gel and PCR Clean-up Midi Complete primer removal down to 65 bp using NucleoSpin Gel and PCR Clean-up Midi. PCR products of 252, 164, 100, and 65 bp were spiked with primers and purified with NucleoSpin Gel and PCR Clean-up Midi. Elution was performed using 400 µl of elution buffer. Shown in the image are the samples prior to (Lane 1) and after (Lane 2) PCR purification on a 2% TAE agarose gel. Back Gel extraction using NucleoSpin Gel and PCR Clean-up Midi Gel extraction using NucleoSpin Gel and PCR Clean-up Midi. pGEM T Easy vectors containing two different inserts were digested using EcoRI and analyzed by electrophoresis. Digested inserts and vectors were cut out of the gel and purified with NucleoSpin Gel and PCR Clean-up Midi. Gel purification was analyzed on a 1% TAE agarose gel. Samples in each lane are as follows: 1. pGEM T Easy with 645-bp insert prior to digestion 2. pGEM T Easy vector backbone and 645-bp insert after digestion 3. 645-bp insert after gel extraction 4. pGEM T Easy vector backbone after gel extraction 5. pGEM T Easy with 982-bp insert prior to digestion 6. pGEM T Easy vector backbone and 982-bp insert after digestion 7. 982-bp insert after gel extraction 8. pGEM T Easy vector backbone after gel extraction
740609.50	NucleoSpin® Gel and PCR Clean-Up	50 Preps	$85.00
NucleoSpin Gel and PCR Clean-up (50) 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).
740609.250	NucleoSpin® Gel and PCR Clean-Up	250 Preps	$389.00
NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).
740609.240C	NucleoSpin® Gel and PCR Clean-Up	240 Preps	$389.00
250 prep clean-up Kit for use on QIAcube includes NucleoSpin® Gel and PCR Clean-up Columns, buffers. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like
740610.20	NucleoSpin® Gel and PCR Clean-up Maxi	20 Preps	$308.00
The NucleoSpin Gel & PCR Clean-up Maxi kit includes spin columns, collection tubes, and buffers for purification of DNA from PCR reactions or agarose gels. The kit enables recovery of DNA fragments ranging in size from 50 bp–20 kb, and can be used to process up to 10 ml of PCR reaction mixture or 10 g of agarose gel in one prep. The binding capacity of the maxi spin column is 250 µg, and purified DNA is typically eluted in a volume of 1 ml. The kit employs a user-friendly workflow that enables processing of 6 samples in ~30 minutes. Cat. # 740610.20 includes sufficient quantities of reagents and materials for performing 20 preps. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents

Overview

One kit for PCR cleanup and gel extraction
Highly flexible—kit can be used for:
- Small to large fragments (50 bp to 20 kb)
- Cleanup from agarose or polyacrylamide gels
- Low and standard elution volumes—elute in as little as 15 µl (mini), 200 µl (midi), or 1 ml (maxi)
- Restriction digest cleanup
- Samples containing detergent (kit has no detergent sensitivity)
Fast—no isopropanol precipitation step for PCR purification
Binding buffer with pH indicator for confirmation of optimal kit performance
Effective removal of primers

More Information

	NucleoSpin Gel and PCR Clean-Up	NucleoSpin Gel and PCR Clean-up Midi	NucleoSpin Gel and PCR Clean-up Maxi
Technology	Silica membrane	Silica membrane	Silica membrane
Format	Mini spin columns	Midi spin columns	Maxi spin columns
Starting material	<400 µl PCR reaction mixture <400 mg TAE/TBE agarose gel	<4 ml PCR reaction mixture <4 g TAE/TBE agarose gel	<10 ml PCR reaction mixture <10 g TAE/TBE agarose gel
Fragment size	50 bp–20 kb	50 bp–20 kb	50 bp–20 kb
Typical recovery	70–95%	70–95%	70–95%
A_260/280	1.8–1.9	1.75–1.85	1.75–1.85
Elution volume	15–30 µl	200–400 µl	1 ml
Preparation time	10 min/6 preps	25 min/6 preps	30 min/6 preps
Binding capacity	25 µg	75 µg	250 µg

Applications

Purified DNA is suitable for:

Sequencing
Ligation
Labeling
Cloning
Restriction enzyme digestion

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

DNA cleanup: two applications in one kit

The NucleoSpin Gel and PCR Clean-Up procedure is the easiest way to purify DNA fragments from agarose gels or PCR reactions. The kit includes one buffer for both applications, which contains a pH indicator that displays the correct pH for optimal kit performance. The purification procedure for samples from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities.

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PCR cleanup and gel extraction kits

Notice to purchaser

Complete primer removal down to 65 bp using NucleoSpin Gel and PCR Clean-up Midi

Gel extraction using NucleoSpin Gel and PCR Clean-up Midi

Notice to purchaser

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

Notice to purchaser

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

Notice to purchaser

Notice to purchaser

Overview

More Information

Applications

Additional product information