PCR cleanup and gel-extraction kits
With the NucleoSpin Gel and PCR Clean-Up kits, you get two applications in one. The supplied column, which is suitable for gel extraction as well as PCR purification, binds DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded directly onto the NucleoSpin Gel and PCR Clean-Up kit column, only a single wash step using Buffer NT3, an optimized buffer, is necessary—providing rapid DNA purification. You can isolate fragments from 50 bp–20 kb in minimal elution volumes (as low as 15 µl for the mini kit, and 200 µl for the midi kit) with a recovery of 70–95%.
With the NucleoSpin Gel and PCR Clean-Up kits, you get two applications in one. The supplied column, which is suitable for gel extraction as well as PCR purification, binds DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded directly onto the NucleoSpin Gel and PCR Clean-Up kit column, only a single wash step using Buffer NT3, an optimized buffer, is necessary—providing rapid DNA purification. You can isolate fragments from 50 bp–20 kb in minimal elution volumes (as low as 15 µl for the mini kit, and 200 µl for the midi kit) with a recovery of 70–95%. Downstream applications include sequencing, ligation, labeling, cloning, and restriction enzyme digestion.
The NucleoSpin Gel and PCR Clean-Up kit was formerly known as the NucleoSpin Extract II kit.
Overview
- One kit for PCR cleanup and gel extraction
- Highly flexible—kit can be used for:
- Small to large fragments (50 bp to 20 kb)
- Low and standard elution volumes—elute in as little as 15 µl (mini) or 200 µl (midi)
- Restriction-digest cleanup
- Samples containing detergent (kit has no detergent sensitivity)
- Fast—no isopropanol precipitation step for PCR purification
- Effective removal of primers
More Information
| NucleoSpin Gel and PCR Clean-Up (Cat # 740609.50, 740609.250, 740609.240C) | NucleoSpin Gel and PCR Clean-Up Midi (Cat # 740986.20) | |
| Technology | Silica membrane | Silica membrane |
| Format | Mini spin columns | Midi spin columns |
| Starting material | <400 µl PCR reaction mixture <400 mg TAE/TBE agarose gel |
<4 ml PCR reaction mixture <4 g TAE/TBE agarose gel |
| Fragment size | 50 bp–20 kb | 50 bp–20 kb |
| Typical recovery | 70–95% | 70–95% |
| A260/280 | 1.8–1.9 | 1.75–1.85 |
| Elution volume | 15–30 µl | 200–400 µl |
| Preparation time | 10 min/6 preps | 25 min/6 preps |
| Binding capacity | 25 µg | 75 µg |
Applications
Purified DNA is suitable for:
- Sequencing
- Ligation
- Labeling
- Cloning
- Restriction enzyme digestion
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
DNA cleanup: two applications in one kit
The NucleoSpin Gel and PCR Clean-Up procedure is the easiest way to purify DNA fragments from agarose gels or PCR reactions. The kit includes one buffer for both applications, which contains a pH indicator that displays the correct pH for optimal kit performance. The purification procedure for samples from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities.
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