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Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder

Endotoxin-free plasmid DNA maxipreps—NucleoBond Xtra Maxi EF/Maxi Plus EF

Transfection-grade plasmid purification kits

NucleoBond Xtra EF kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA, Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included special column filter, designed for high filter flow rates and rapid lysate clearing—no centrifugation is required.

NucleoBond Xtra EF kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA, Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included special column filter, designed for high filter flow rates and rapid lysate clearing—no centrifugation is required. Additionally, NucleoBond Xtra has improved silica material with higher binding capacity, leading to typical yields of 400 μg (Midi) and 1000 μg (Maxi). 

NucleoBond Xtra Finalizers: The finalizers, provided with the Plus kits, replace the time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

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Cat. # Product Size License Quantity Details
740426.50 NucleoBond® Xtra Maxi Plus EF 50 Preps USD $2130.00

NucleoBond Xtra Maxi Plus EF 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits
NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

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The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

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740426.50: NucleoBond Xtra Maxi Plus EF

740426.50: NucleoBond Xtra Maxi Plus EF
740426.10 NucleoBond® Xtra Maxi Plus EF 10 Preps USD $447.00

NucleoBond Xtra Maxi Plus EF 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits
NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

Back

740426.10: NucleoBond Xtra Maxi Plus EF

740426.10: NucleoBond Xtra Maxi Plus EF
740424.50 NucleoBond® Xtra Maxi EF 50 Preps USD $1885.00

NucleoBond Xtra Maxi EF 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

Back

740424.50: NucleoBond Xtra Maxi EF

740424.50: NucleoBond Xtra Maxi EF
740424.10 NucleoBond® Xtra Maxi EF 10 Preps USD $378.00

NucleoBond Xtra Maxi EF 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

740424.10: NucleoBond Xtra Maxi EF

740424.10: NucleoBond Xtra Maxi EF

Technical notes and data highlighting the performance of our nucleic acid purification kits Learning center
Transfection reagents Transfection products
Transfection grade plasmid purifcation kits Transfection-grade plasmid kits

Overview

  • Xtra fast—reduce prep time by up to 68% (103 minutes for Maxi EF and 48 minutes for Maxi Plus EF)
  • Xtra high yields—obtain up to 100% more transfection-grade plasmid DNA. Typical yield: up to 1,000 µg
  • Xtra efficient endotoxin removal—obtain DNA with less than 0.05 EU/µg
  • Xtra flexible—available with or without the NucleoBond Finalizer (for ultrafast purification)

More Information

NucleoBond Xtra Maxi EF

NucleoBond Xtra Maxi Plus EF

Technology

Anion exchange

Anion exchange

Format

Maxi gravity-flow columns

Maxi gravity-flow columns

Lysate clarification

Column filters (included)

Column filters (included)

Starting material

<600 ml (high copy)
<1,200 ml (low copy)

<600 ml (high copy)
<1,200 ml (low copy)

Vector size

<300 kb

<300 kb

Typical yield

>1,000 µg

>1,000 µg

A260/280

1.80–1.95

1.80–1.95

Endotoxin level

<0.05 EU/µg DNA

<0.05 EU/µg DNA

Isopropanol precipitation

Centrifugation

NucleoBond Finalizer

Preparation time

103 min/4 preps

48 min/4 preps

Applications

Purified DNA is suitable for:

  • Transfection of highly sensitive cells
  • Lentivirus production

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


plasmid transfection

Tip for a successful transfection—remove endotoxins!

Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.

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