Maxipreps for plasmid DNA purification—NucleoBond Xtra Maxi/Maxi Plus
NucleoBond Xtra Maxi and Maxi Plus are a new generation of anion-exchange plasmid purification kits based on the patented NucleoBond technology. Typical yields of >1,000 µg of ultra-pure plasmid DNA can be obtained in about half the time compared to other maxi kits based on anion-exchange chromatography. The entire plasmid-purification procedure takes 80 minutes with NucleoBond Xtra Maxi and 34 minutes with NucleoBond Xtra Maxi Plus.
NucleoBond Xtra Maxi and Maxi Plus are a new generation of anion-exchange plasmid purification kits based on the patented NucleoBond technology. Typical yields of >1,000 µg of ultra-pure plasmid DNA can be obtained in about half the time compared to other maxi kits based on anion-exchange chromatography. The entire plasmid-purification procedure takes 80 minutes with NucleoBond Xtra Maxi and 34 minutes with NucleoBond Xtra Maxi Plus.
NucleoBond Xtra Filters: Specially designed column filters are supplied inserted in the NucleoBond Xtra Columns, allowing a convenient and time-saving parallel clarification of bacterial lysate and loading onto the column—minimizing the risk of clogging.
NucleoBond Xtra Columns: Enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for a fast purification. The improved silica material is based on the anion-exchange group MAE.
NucleoBond Xtra Buffers: Optimized buffer compositions lead to improved alkaline lysis and increased column flow rates. Get more DNA in a shorter processing time.
NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.
Overview
- Xtra fast—reduce prep time by up to 60%
- Xtra high yields—obtain up to 100% more transfection-grade DNA. Typical yield: 1,000 µg
- Xtra purity—obtain the highest purity via proven anion-exchange technology
- Xtra flexible—choose from Midiprep/Maxiprep, with or without NucleoBond Finalizer (for ultra-fast purification)
More Information
| NucleoBond Xtra Maxi | NucleoBond Xtra Maxi Plus | |
| Technology | Anion exchange | Anion exchange |
| Format | Maxi | Maxi |
| Lysate clarification | Column filters | Column filters |
| Starting material | < 600 ml (high-copy-number) < 1,200 ml (low-copy-number) |
< 600 ml (high-copy-number) < 1,200 ml (low-copy-number) |
| Vector size | <300 kb | <300 kb |
| Typical yield | >1,000 µg | >1,000 µg |
| A260/280 | 1.80–1.95 | 1.80–1.95 |
| Isopropanol precipitation | Centrifugation | NucleoBond Finalizer |
| Preparation time | 80 min/4 preps | 34 min/4 preps |
Applications
- Transfecting sensitive cells
- Sequencing
- Cloning
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
See what our customers are saying about NucleoBond Xtra Maxi!
"NucleoBond Xtra Maxi kit gave me an excellent yield of plasmid. The average yield of plasmids were 1.5ug/ul. I may recommend this product to my colleague if he/she has a trouble in the preparation of plasmids."
-Sungpil Cho, UNIVERSITY OF UTAH
"Very impressive how easy to avoid clogs and saved lots of time!"
-Jenny Shu,WASHINGTON UNIV IN ST LOUIS
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