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Plasmid purification kits

Maxipreps for plasmid DNA purification—NucleoBond Xtra Maxi/Maxi Plus

Ultra-fast, transfection-grade plasmid DNA by gravity flow, high yield >1 mg

NucleoBond Xtra Maxi and Maxi Plus are a new generation of anion-exchange plasmid purification kits based on the patented NucleoBond technology. Typical yields of >1,000 µg of ultra-pure plasmid DNA can be obtained in about half the time compared to other maxi kits based on anion-exchange chromatography. The entire plasmid-purification procedure takes 80 minutes with NucleoBond Xtra Maxi and 34 minutes with NucleoBond Xtra Maxi Plus.

NucleoBond Xtra Filters: Specially designed column filters are supplied inserted in the NucleoBond Xtra Columns, allowing a convenient and time-saving parallel clarification of bacterial lysate and loading onto the column—minimizing the risk of clogging.

NucleoBond Xtra Columns: Enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for a fast purification. The improved silica material is based on the anion-exchange group MAE.

NucleoBond Xtra Buffers: Optimized buffer compositions lead to improved alkaline lysis and increased column flow rates. Get more DNA in a shorter processing time.

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

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Cat. #	Product	Size	Price
740414.50	NucleoBond® Xtra Maxi	50 Preps	$1089.00
NucleoBond Xtra Maxi (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Resources Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.
740414.100	NucleoBond® Xtra Maxi	100 Preps	$2030.00
Use NucleoBond Xtra Maxi and Maxi Plus to purify plasmid DNA in 30 min without the use of vacuum or centrifugation, Typical yield >1,000 µg of ultra-pure plasmid DNA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.
740414.10	NucleoBond® Xtra Maxi	10 Preps	$231.00
NucleoBond Xtra Maxi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Resources Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.
740424.50	NucleoBond® Xtra Maxi EF	50 Preps	$1618.00
NucleoBond Xtra Maxi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.
740424.10	NucleoBond® Xtra Maxi EF	10 Preps	$324.00
NucleoBond Xtra Maxi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.
740416.50	NucleoBond® Xtra Maxi Plus	50 Preps	$1217.00
NucleoBond Xtra Maxi Plus (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Resources Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.
740416.10	NucleoBond® Xtra Maxi Plus	10 Preps	$254.00
NucleoBond Xtra Maxi Plus (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Resources Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.
740426.50	NucleoBond® Xtra Maxi Plus EF	50 Preps	$1825.00
NucleoBond Xtra Maxi Plus EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.
740426.10	NucleoBond® Xtra Maxi Plus EF	10 Preps	$383.00
NucleoBond Xtra Maxi Plus EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

Overview

Xtra fast—reduce prep time by up to 60%
Xtra high yields—obtain up to 100% more transfection-grade DNA. Typical yield: 1,000 µg
Xtra purity—obtain the highest purity via proven anion-exchange technology
Xtra flexible—choose from Midiprep/Maxiprep, with or without NucleoBond Finalizer (for ultra-fast purification)

More Information

	NucleoBond Xtra Maxi	NucleoBond Xtra Maxi Plus
Technology	Anion exchange	Anion exchange
Format	Maxi	Maxi
Lysate clarification	Column filters	Column filters
Starting material	< 600 ml (high-copy-number) < 1,200 ml (low-copy-number)	< 600 ml (high-copy-number) < 1,200 ml (low-copy-number)
Vector size	<300 kb	<300 kb
Typical yield	>1,000 µg	>1,000 µg
A_260/280	1.80–1.95	1.80–1.95
Isopropanol precipitation	Centrifugation	NucleoBond Finalizer
Preparation time	80 min/4 preps	34 min/4 preps

Applications

Transfecting sensitive cells
Sequencing
Cloning

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

See what our customers are saying about NucleoBond Xtra Maxi!

"NucleoBond Xtra Maxi kit gave me an excellent yield of plasmid. The average yield of plasmids were 1.5ug/ul. I may recommend this product to my colleague if he/she has a trouble in the preparation of plasmids."
-Sungpil Cho, UNIVERSITY OF UTAH

"Very impressive how easy to avoid clogs and saved lots of time!"
-Jenny Shu,WASHINGTON UNIV IN ST LOUIS

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Maxipreps for plasmid DNA purification—NucleoBond Xtra Maxi/Maxi Plus

Notice to purchaser

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

Notice to purchaser

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

Notice to purchaser

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

Notice to purchaser

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

Notice to purchaser

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

Notice to purchaser

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

Notice to purchaser

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

Notice to purchaser

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

Notice to purchaser

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

Overview

More Information

Applications

Additional product information

See what our customers are saying about NucleoBond Xtra Maxi!