BAC DNA isolation in a 96-well plate format—NucleoSpin 96 Flash
The NucleoSpin 96 Flash kit lets you rapidly isolate BAC DNA from bacterial cultures using a high-throughput method. Process samples in a 96-well format using either a vacuum manifold or a tabletop centrifuge. Obtain BAC yields of <1 µg from 1.3–3.9 ml of E. coli culture or up to 8 µg of high-copy-number plasmid from 1.1–1.3 ml of E. coli culture.
Overview
- Suitable for high-throughput purification of large constructs (BACs, cosmids)
- Vectors up to 300 kb can be purified
- Manual or automated processing
More Information
| Technology | Alkaline lysis with subsequent filtration and precipitation |
| Format | 96-well plates |
| Processing | Manual or automated; vacuum or centrifugation |
| Lysate clarification | 96-well plate filter plates |
| Starting material | 1.1–1.3 ml of E. coli culture 1.3–3.9 ml of E. coli culture (BACs) |
| Vector size | <250 kb |
| Typical yield | 8 µg from 1.1–1.3 ml of E. coli culture (high-copy plasmid) <1 µg from 1.3–3.9 ml of E. coli culture (BACs) |
| Preparation time | 90 min/2 plates |
Applications
The purified DNA is suitable for:
- Sequencing
- PCR
- Restriction digest
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tip for a successful transfection—remove endotoxins!
Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.
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