Endotoxin-free plasmid midiprep DNA purification—NucleoBond Xtra Midi EF/Midi Plus EF
NucleoBond PC EF Kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA. Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included special column filter, designed for high filter flow rates, and rapid lysate clearing, no centrifugation is required.
NucleoBond PC EF Kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA. Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included special column filter, designed for high filter flow rates, and rapid lysate clearing, no centrifugation is required. Additionally NucleoBond Xtra has improved silica material with higher binding capacity, leading to typical yields of 400 μg (Midi) and 1000 μg (Maxi).
NucleoBond Xtra Finalizers: The finalizers, provided with the Plus kits, replace the time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.
Overview
- Xtra fast—reduce prep time by up to 68% (100 minutes for Midi EF and 45 minutes for Midi Plus EF)
- Xtra high yields—obtain up to 100% more transfection-grade plasmid DNA. Typical yield: 250–500 µg
- Xtra efficient endotoxin removal—obtain DNA with less than 0.05 EU/µg
- Xtra flexible—with or without NucleoBond Finalizer (for ultrafast purification)
More Information
| NucleoBond Xtra Midi EF | NucleoBond Xtra Midi Plus EF | |
| Technology |
Anion exchange |
Anion exchange |
| Format |
Midi gravity-flow columns |
Midi gravity-flow columns |
| Lysate clarification |
Column filters (included) |
Column filters (included) |
| Starting material |
<200 ml (high copy) |
<200 ml (high copy) |
| Vector size |
<300 kb |
<300 kb |
| Typical yield |
>250 µg |
>250 µg |
| A260/280 |
1.80–1.95 |
1.80–1.95 |
| Endotoxin level |
<0.05 EU/µg DNA |
<0.05 EU/µg DNA |
| Isopropanol precipitation |
Centrifugation |
NucleoBond Finalizer |
| Preparation time |
100 min/4 preps |
45 min/4 preps |
Applications
Purified DNA is suitable for:
- Transfection of highly sensitive cells
- Lentivirus production
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tip for a successful transfection—remove endotoxins!
Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.
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