NucleoMag Plasmid

Increase plasmid DNA isolation throughput and achieve consistent results without the need for constant monitoring. NucleoMag Plasmid enables semi-automation of plasmid DNA isolation on magnetic rod systems, such as the IsoPure Mini or the IsoPure 96. The resulting high-quality plasmid DNA is suitable for typical downstream applications such as sequencing, cloning, PCR, transformation, transfection, and ligation.

Cat. #	Product	Size	Price
744750.1	NucleoMag® Plasmid	96 Preps	USD $185.00
The NucleoMag Plasmid kit is designed for the automated isolation of plasmid DNA from bacterial lysates using magnetic rod systems. Following lysis and clarification of the sample material, NucleoMag M-Beads are used to bind plasmid DNA. After the washing steps, highly pure DNA is eluted from the beads. Lysis and clarification buffers, NuceloMag M-Beads, binding buffers, and wash buffers are included. With the NucleoMag Plasmid workflow, the bacterial lysate can be cleared via centrifugation before loading into a plate for automation on a magnetic rod system. Alternatively, lysate clearing can be automated using NucleoMag Clearing Beads. NucleoMag Clearing Beads are not included with this kit and must be purchased separately. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
744750.4	NucleoMag® Plasmid	4 x 96 Preps	USD $651.00
The NucleoMag Plasmid kit is designed for the automated isolation of plasmid DNA from bacterial lysates using magnetic rod systems. Following lysis and clarification of the sample material, NucleoMag M-Beads are used to bind plasmid DNA. After the washing steps, highly pure DNA is eluted from the beads. Lysis and clarification buffers, NuceloMag M-Beads, binding buffers, and wash buffers are included. With the NucleoMag Plasmid workflow, the bacterial lysate can be cleared via centrifugation before loading into a plate for automation on a magnetic rod system. Alternatively, lysate clearing can be automated using NucleoMag Clearing Beads. NucleoMag Clearing Beads are not included with this kit and must be purchased separately. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
744751.1	NucleoMag® Clearing Beads	96 Preps	USD $59.00
NucleoMag Clearing Beads are for bacterial lysate clarification during plasmid DNA isolation with the NucleoMag Plasmid kit. They offer a simple solution for automated lysate clarification on magnetic rod systems and can replace centrifugation. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents

Overview

Save time and energy by automating plasmid DNA isolation
Choose between centrifugation-based or magnetic bead-based lysate clarification
Achieve reliable and consistent results with yields of up to 50 µg per sample

More Information

Format	Magnetic beads
Vector Size	≤25 kb
Typical yield	1–50 μg
Typical purity	A₂₆₀/A₂₈₀ ≥1.8 A₂₆₀/A₂₃₀ ≥2.0
Sample amount	≤5 ml E. coli culture
Lysate clarification	Centrifugation or magnetic beads
Typical endotoxin levels	Lysate clarification via NucleoMag Clearing Beads: ≤50 EU/µg Lysate clarification via centrifugation with 3 wash steps: ≤50 EU/µg Lysate clarification via centrifugation with 4 wash steps: ≤10 EU/µg
Automated platform compatibility	Magnetic rod system platforms such as the IsoPure Mini. NOTE: Not intended for use with liquid handling systems. For plasmid DNA isolation on liquid handling systems, we offer NucleoSpin 8 Plasmid and NucleoSpin 96 Plasmid.
Elution volume	50–200 µl
Processing time	~50 min on the IsoPure Mini

Applications

Sequencing
Cloning
PCR
Transformation
Transfection
In vitro transcription
Ligation and restriction digest

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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NucleoMag Plasmid

Notice to purchaser

Notice to purchaser

Notice to purchaser

Overview

More Information

Applications

Additional product information