BAC DNA purification—NucleoBond Xtra BAC
NucleoBond Xtra BAC provides second-generation anion exchange for large-construct plasmid DNA. The high selectivity and specific characteristics of the optimized silica matrix make this anion exchange kit ideal for efficiently purifying very large constructs, such as P1, BACs, and PACs. For example, using the NucleoBond Xtra BAC kit, it takes just 75 minutes to isolate BAC DNA free of all types of RNA, proteins, metabolites, dyes, and carbohydrates.
NucleoBond Xtra BAC provides second-generation anion exchange for large-construct plasmid DNA. The high selectivity and specific characteristics of the optimized silica matrix make this anion exchange kit ideal for efficiently purifying very large constructs, such as P1, BACs, and PACs. For example, using the NucleoBond Xtra BAC kit, it takes just 75 minutes to isolate BAC DNA free of all types of RNA, proteins, metabolites, dyes, and carbohydrates. This kit completely removes impurities which can act as strong inhibitors for enzymatic processing, transfections, transcriptions, etc., even when present in small amounts.
LyseControl, a blue pH indicator reagent present in the lysis buffer used in the NucleoBond Xtra BAC procedure, ensures complete and efficient alkaline lysis, providing optimal yields. Upon addition of lysis buffer to the resuspended cells, the cell suspension turns blue. To avoid contamination with genomic DNA, the blue solution is gently inverted up to five times until a homogeneous and 100% colorless solution is achieved. After a five-minute incubation, neutralization buffer is added and LyseControl turns colorless. Traces of blue LyseControl indicate insufficient mixing. Using LyseControl ensures sufficient mixing and complete neutralization, which are necessary to renature plasmid DNA, precipitate proteins/gDNA, and remove SDS, which inhibits plasmid binding.
- Optimized column design—complete one prep in approximately 75 minutes
- Column filter included—allows parallel lysate clearing and loading onto the column
- Optimized silica matrix—yields up to 150 μg of purified large-construct plasmid DNA
- Transfection-grade plasmid DNA—obtained using proven anion-exchange technology
- LyseControl—visually confirms completion of lysis/neutralization, maximizing yield
|Lysate clarification||Column filters|
|Starting material||250–750 ml|
|Vector size||<300 kb|
|Typical yield||10–100 µg|
|Preparation time||75 min/prep|
|Binding capacity||150 µg|
- Purification of P1, BACs, PACs, and other large constructs from E. coli
- Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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