Plasmid DNA purification using 8-well filter strips—NucleoSpin 8 Plasmid
The NucleoSpin 8 Plasmid and NucleoSpin 8 Plasmid Core Kits are designed to purify up to 20 µg of high-copy plasmids from 1–5 ml of E. coli overnight cultures (preferably grown in LB medium). The NucleoSpin Plasmid Binding Strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.
Bacteria are harvested in an initial centrifugation step. After resuspension of the pelleted bacteria and alkaline cell lysis, the crude bacterial lysates are cleared by centrifugation or loaded onto NucleoSpin Plasmid Filter Strips arranged on the NucleoVac 96 vacuum manifold.
Overview
- Time-saving parallel isolation of plasmid DNA
- Processing of up to 5 ml of bacterial culture
- NucleoSpin 8 Plasmid Filter Strips included for convenient filtration of bacterial lysates
- Processing possible under vacuum
- Innovative MN Wash Plate minimizes risk of cross-contamination
- DNA is ready-to-use for PCR, Southern blotting, or any kind of enzymatic reaction
More Information
| NucleoSpin 8 Plasmid and NucleoSpin 8 Plasmid Core Kit | |
| Technology | Silica membrane |
| Format | 8-well filter strips |
| Processing | Manual and automated, vacuum |
| Lysate clarification | 8-well filter strips |
| Starting material | 1–5 ml E. coli culture |
| Vector size | <15 kb |
| Typical yield | 4–6 µg/ml E. coli culture |
| A260/280 | 1.70–1.85 |
| Elution volume | 75–150 µl |
| Preparation time | 45 min/6 strips |
| Binding capacity | 20 µg |
Applications
- PCR
- Southern blotting
- Any kind of enzymatic reaction
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes
Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold.
Learn more Other plasmid kitsTakara Bio USA, Inc.
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