Megaprep endotoxin-free plasmid DNA purification—NucleoBond PC 2000 EF
NucleoBond PC EF Kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA. Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included NucleoBond Folded Filters—no centrifugation is required. NucleoBond PC 2000 EF is used to purify 500–2000 µg of endotoxin-free plasmid DNA.
Overview
- Endotoxins are efficiently removed (<0.1 EU/µg DNA)
- No additional incubation is required for endotoxin removal
- Purify 2,000 µg from 150–2,000 ml of overnight E. coli culture
- Patented procedure
More Information
| Technology | Anion exchange |
| Format | Mega gravity-flow columns |
| Lysate clarification | Bottle top filters |
| Starting material | 150–2,000 ml |
| Vector size | <300 kb |
| Typical yield | 1,700–2,000 µg |
| A260/280 | 1.80–1.95 |
| Endotoxin level | <0.1 EU/µg |
| Preparation time | 150 min/2 preps |
Applications
Purified DNA is suitable for:
- Transfection of highly sensitive cells
- Lentivirus production
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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