Plasmid miniprep (ultra-fast): NucleoSpin Plasmid EasyPure kit
NucleoSpin Plasmid EasyPure is a high-speed and high-yield miniprep kit that combines the highest plasmid yield with a very fast procedure. The NucleoSpin Plasmid EasyPure columns supplied in the kit provide a binding capacity of 35 µg plasmid DNA and allow reliable yields, even from higher culture volumes. Rapid purification is achieved by using a combined washing and drying step, which allows 6 minipreps to be completed within 14 min.
NucleoSpin Plasmid EasyPure is a high-speed and high-yield miniprep kit that combines the highest plasmid yield with a very fast procedure. The NucleoSpin Plasmid EasyPure columns supplied in the kit provide a binding capacity of 35 µg plasmid DNA and allow reliable yields, even from higher culture volumes. Rapid purification is achieved by using a combined washing and drying step, which allows 6 minipreps to be completed within 14 min.
This simple procedure starts with alkaline lysis of a bacterial culture. The blue LyseControl indicator reagent premixed in the Lysis Buffer allows you to easily visualize completion of neutralization and alkaline lysis. The lysate is then cleared by centrifugation and the supernatant is loaded onto a NucleoSpin Plasmid EasyPure column. Contaminants such as salts, metabolites, nucleases, and soluble macromolecular cellular components are removed by a single washing and drying step.
Overview
- Ultra-fast, small-scale preparation of up to 35 µg of high-purity plasmid DNA
- LyseControl allows visualization of completed alkaline lysis
- Liquid RNase A provides easy handling—no need to dissolve enzyme
- One combined washing and drying step enables ultra-fast purification (6 minipreps in 14 min)
More Information
| Technology | Silica membrane |
| Format | Mini spin columns |
| Starting material | 2–10 ml E. coli culture |
| Vector size | <15 kb |
| Typical yield | 15–30 µg |
| A260/280 | 1.80–1.85 |
| Elution volume | 50 µl |
| Preparation time | 14 min/6 preps |
| Binding capacity | 35 µg |
Applications
- High-copy plasmids from E. coli
- Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
- Plasmid DNA from Gram-positive bacteria and plasmid DNA cleanup from reaction mixtures
- Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tip for a successful transfection—remove endotoxins!
Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.
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