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  • NucleoBond PC 2000
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Home › Products › Nucleic acid purification › Plasmid purification kits › Megaprep and gigaprep › NucleoBond PC 2000

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Plasmid DNA megaprep—NucleoBond PC 2000

Purify up to 2 mg of plasmid DNA in just a few hours without phenol/chloroform extraction

NucleoBond PC 2000 plasmid megaprep kits allow you to purify up to 2,000 µg of plasmid DNA in just a few hours without phenol/chloroform extraction. The kits can be used to purify high- and low-copy-number plasmids, cosmids, BACs, PACs, and YACs grown from bacterial cultures. The resulting plasmids are at least as pure as plasmids isolated by traditional cesium chloride procedures and can be used for the most demanding molecular biology applications, including mammalian transfection, in vitro transcription, and automated sequencing. Also see our NucleoBond Xtra Maxi Kits.

Cat. # Product Size Price License Quantity Details
740576 NucleoBond® PC 2000 5 Preps $234.00

NucleoBond PC 2000 (5) 5 preps for the isolation of up to 2,000 µg of plasmid DNA - NucleoBond AX 2000 Columns, NucleoBond Folded Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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The NucleoBond PC plasmid purification protocol

The NucleoBond PC plasmid purification protocol
The NucleoBond PC plasmid purification protocol.

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Transfection of HeLa cells with NucleoBond-purified plasmid DNA

Transfection of HeLa cells with NucleoBond-purified plasmid DNA
Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.

Mega or giga scale plasmid purification kits Megaprep/gigaprep plasmid kits
Data showing the use of various kits to purify plasmids Plasmid kits
Overviews NucleoSnap tech note

Overview

  • Isolate highly pure plasmid DNA for transfection or sequencing
  • Rapid purification procedure using gravity flow
  • Clear lysates without centrifugation (filters provided with the kit)
  • Highly reproducible purification results

More Information

Technology Anion exchange
Format Megaprep
Lysate clarification Folded filters
Starting material 150–2,000 ml (high-copy-number)
Vector size <300 kb
Typical yield 1,700–2,000 µg
A260/280 1.80–1.95
Preparation time 90–120 min/4–6 preps

Applications

  • Transfecting sensitive cells
  • Sequencing
  • Cloning
  • PCR
  • Restriction analysis

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Isolate up to 700 μg of transfection-grade plasmid DNA in only 35 minutes

Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes

Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold. 

Learn more Other plasmid kits

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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