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  • NucleoSpin Plasmid Transfection-grade
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Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Isolate up to 700 μg of transfection-grade plasmid DNA in only 35 minutes NucleoSnap Plasmid Midi
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Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Isolate up to 700 μg of transfection-grade plasmid DNA in only 35 minutes NucleoSnap Plasmid Midi
Product Overview

NucleoSnap Plasmid Midi

  • Isolate 250 μg of plasmid DNA in only 35 minutes
  • Transfection-grade plasmid DNA for sensitive downstream applications
  • New column design (snap-off column) for vacuum processing of large sample volumes

The NucleoSnap Plasmid Midi kits are designed for the rapid purification of highly pure plasmid DNA from up to 50 ml of a standard E. coli overnight culture. Plasmid DNA isolated with this kit is suitable for all common downstream applications including enzymatic digestion, cloning, sequencing, PCR amplification, transformation, and transfection.

snap-off column design

At-a-glance Application data Vacuum processing

At-a-glance  

Technology Silica-membrane technology
Processing Vacuum processing (using NucleoVac 24 Vacuum Manifold), centrifugation for elution
Format Snap-off column
Endotoxin level <50 EU/µg DNA (EU = Endotoxin Units)
Sample material Up to 50 ml of E. coli culture (OD600 = 5)
Lysate clarification NucleoSpin Plasmid Filter Columns (2-min centrifugation)
Vector size ≤25 kbp
Typical yield 250 µg (50-ml culture, OD600 = 4, high-copy plasmid)
A260/A280 1.8–1.9
Elution volume 200–500 µl
Preparation time 35 min/6 preps
Binding capacity 1.5 mg

Application data  

Comparison of cell viability after transfection with plasmid DNA purified by silica, NucleoSnap Plasmid Midi, and anion-exchange columns.

High cell viability of eukaryotic cells. Huh-7 cells were transfected with 2.5 μg of plasmid DNA (pCMV-GFP, kindly provided by PlasmidFactory GmbH & Co. KG, Bielefeld, Germany). The plasmid DNA was prepared with a standard silica mini spin prep (such as NucleoSpin Plasmid), NucleoSnap Plasmid Midi and an anion-exchange DNA isolation kit (such as NucleoBond Xtra Midi EF).

Endotoxin levels appropriate for individual applications

Endotoxin levels appropriate for individual applications. A quantitative chromogenic LAL-test was used to assess endotoxin content. As indicated, the content of endotoxin is strongly dependent on the technology of plasmid purification. Low endotoxin levels were detected after purification with NucleoSnap Plasmid Midi resulting in a plasmid solution directly appropriate for transfection of common cells.

Vacuum processing  

Up to 24 samples can be processed in parallel with our NucleoVac 24 Vacuum Manifold, and no centrifugation steps are required during binding and washing steps.

NucleoVac 24 Vacuum Manifold

Ordering Information

Cat. # Product Size License Quantity Details
740300.50 NucleoSnap® cfDNA 50 Preps USD $965.00

The NucleoSnap cfDNA kit is designed for isolating circulating cell-free DNA (cfDNA) from plasma.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The NucleoSnap (snap-off) column splits into mini spin column and funnel components

The NucleoSnap (snap-off) column splits into mini spin column and funnel components
The NucleoSnap (snap-off) column splits into mini spin column and funnel components.

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740300.50: NucleoSnap DNA Plasma

740300.50: NucleoSnap DNA Plasma
740300.10 NucleoSnap® cfDNA 10 Preps USD $222.00

The NucleoSnap cfDNA kit is designed for isolating circulating cell-free DNA (cfDNA) from plasma.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoSnap (snap-off) column splits into mini spin column and funnel components

The NucleoSnap (snap-off) column splits into mini spin column and funnel components
The NucleoSnap (snap-off) column splits into mini spin column and funnel components.

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740300.10: NucleoSnap DNA Plasma

740300.10: NucleoSnap DNA Plasma
740299 NucleoVac 24 Vacuum Manifold Each USD $282.00

The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. The silica columns (e.g., NucleoSpin and NucleoSnap columns) can be attached to the upper Luer openings of the vacuum manifold. The binding and washing steps of the NucleoSpin or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling time-saving procedures (NucleoSpin products) and processing of large sample volumes (NucleoSnap products).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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Setting up the NucleoVac 24 Vacuum Manifold

Setting up the NucleoVac 24 Vacuum Manifold
Setting up the NucleoVac 24 Vacuum Manifold. (1) Rubber closing plug. (2) Tubing connection. (3) Upper opening.

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Processing columns with the NucleoVac 24 Vacuum Manifold

Processing columns with the NucleoVac 24 Vacuum Manifold
Processing columns with the NucleoVac 24 Vacuum Manifold. (4) NucleoSpin column. (5) Luer opening. (6) NucleoVac Mini Adapter. (7) NucleoVac Stopcock. (8) Luer plug.

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740299: NucleoVac 24 Vacuum Manifold

740299: NucleoVac 24 Vacuum Manifold
740297.100 NucleoVac Mini Adapter 100 Each USD $104.00

The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. The silica columns (e.g., NucleoSpin and NucleoSnap columns) can be attached to the upper Luer openings of the vacuum manifold. The binding and washing steps of the NucleoSpin or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling time-saving procedures (NucleoSpin products) and processing of large sample volumes (NucleoSnap products).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Setting up the NucleoVac 24 Vacuum Manifold

Setting up the NucleoVac 24 Vacuum Manifold
Setting up the NucleoVac 24 Vacuum Manifold. (1) Rubber closing plug. (2) Tubing connection. (3) Upper opening.

Back

Processing columns with the NucleoVac 24 Vacuum Manifold

Processing columns with the NucleoVac 24 Vacuum Manifold
Processing columns with the NucleoVac 24 Vacuum Manifold. (4) NucleoSpin column. (5) Luer opening. (6) NucleoVac Mini Adapter. (7) NucleoVac Stopcock. (8) Luer plug.

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740297.100: NucleoVac Mini Adapter

740297.100: NucleoVac Mini Adapter
740298.24 NucleoVac Stopcocks 24 Each USD $151.00

The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. The silica columns (e.g., NucleoSpin and NucleoSnap columns) can be attached to the upper Luer openings of the vacuum manifold. The binding and washing steps of the NucleoSpin or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling time-saving procedures (NucleoSpin products) and processing of large sample volumes (NucleoSnap products).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Setting up the NucleoVac 24 Vacuum Manifold

Setting up the NucleoVac 24 Vacuum Manifold
Setting up the NucleoVac 24 Vacuum Manifold. (1) Rubber closing plug. (2) Tubing connection. (3) Upper opening.

Back

Processing columns with the NucleoVac 24 Vacuum Manifold

Processing columns with the NucleoVac 24 Vacuum Manifold
Processing columns with the NucleoVac 24 Vacuum Manifold. (4) NucleoSpin column. (5) Luer opening. (6) NucleoVac Mini Adapter. (7) NucleoVac Stopcock. (8) Luer plug.

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740298.24: NucleoVac Stopcocks

740298.24: NucleoVac Stopcocks
740641 NucleoVac™ Vacuum Regulator Each USD $194.00

NucleoVac 96 Vacuum Regulator for use with NucleoVac 96 Vacuum Manifold, for controlling of vacuum

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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740641: NucleoVac Vacuum Regulator

740641: NucleoVac Vacuum Regulator


Tips for successully using transfection reagents

Tips for a successful transfection

This guide will help you select the right reagent and find useful tips for designing a successful transfection experiment. Tips include: how to plate your cells at the right density, how to make sure you are using plasmid DNA of sufficiently high quality, and how to assess transfection efficiency.

Transfection tips Plasmid transfection reagents

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