| Technology | Silica-membrane technology |
| Processing | Vacuum processing (using NucleoVac 24 Vacuum Manifold), centrifugation for elution |
| Format | Snap-off column |
| Endotoxin level | <50 EU/µg DNA (EU = Endotoxin Units) |
| Sample material | Up to 50 ml of E. coli culture (OD600 = 5) |
| Lysate clarification | NucleoSpin Plasmid Filter Columns (2-min centrifugation) |
| Vector size | ≤25 kbp |
| Typical yield | 250 µg (50-ml culture, OD600 = 4, high-copy plasmid) |
| A260/A280 | 1.8–1.9 |
| Elution volume | 200–500 µl |
| Preparation time | 35 min/6 preps |
| Binding capacity | 1.5 mg |
Product Overview
NucleoSnap Plasmid Midi
- Isolate 250 μg of plasmid DNA in only 35 minutes
- Transfection-grade plasmid DNA for sensitive downstream applications
- New column design (snap-off column) for vacuum processing of large sample volumes
The NucleoSnap Plasmid Midi kits are designed for the rapid purification of highly pure plasmid DNA from up to 50 ml of a standard E. coli overnight culture. Plasmid DNA isolated with this kit is suitable for all common downstream applications including enzymatic digestion, cloning, sequencing, PCR amplification, transformation, and transfection.
At-a-glance
Application data
High cell viability of eukaryotic cells. Huh-7 cells were transfected with 2.5 μg of plasmid DNA (pCMV-GFP, kindly provided by PlasmidFactory GmbH & Co. KG, Bielefeld, Germany). The plasmid DNA was prepared with a standard silica mini spin prep (such as NucleoSpin Plasmid), NucleoSnap Plasmid Midi and an anion-exchange DNA isolation kit (such as NucleoBond Xtra Midi EF).
Endotoxin levels appropriate for individual applications. A quantitative chromogenic LAL-test was used to assess endotoxin content. As indicated, the content of endotoxin is strongly dependent on the technology of plasmid purification. Low endotoxin levels were detected after purification with NucleoSnap Plasmid Midi resulting in a plasmid solution directly appropriate for transfection of common cells.
Vacuum processing
Up to 24 samples can be processed in parallel with our NucleoVac 24 Vacuum Manifold, and no centrifugation steps are required during binding and washing steps.
Ordering Information
Tips for a successful transfection
This guide will help you select the right reagent and find useful tips for designing a successful transfection experiment. Tips include: how to plate your cells at the right density, how to make sure you are using plasmid DNA of sufficiently high quality, and how to assess transfection efficiency.
Transfection tips Plasmid transfection reagentsTakara Bio USA, Inc.
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