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Transfection-grade plasmid DNA for sensitive downstream applications
New column design (snap-off column) for vacuum processing of large sample volumes
The NucleoSnap Plasmid Midi kits are designed for the rapid purification of highly pure plasmid DNA from up to 50 ml of a standard E. coli overnight culture. Plasmid DNA isolated with this kit is suitable for all common downstream applications including enzymatic digestion, cloning, sequencing, PCR amplification, transformation, and transfection.
Up to 24 samples can be processed in parallel with our NucleoVac 24 Vacuum Manifold, and no centrifugation steps are required during binding and washing steps.
Tips for a successful transfection
This guide will help you select the right reagent and find useful tips for designing a successful transfection experiment. Tips include: how to plate your cells at the right density, how to make sure you are using plasmid DNA of sufficiently high quality, and how to assess transfection efficiency.