NucleoSpin RNA Blood kits provide high yields of high-quality total RNA using a simple, direct blood cell lysis procedure which is very effective at room temperature, without the need for an upstream selective erythrocyte lysis at 4°C (Figure 1). These kits are available in convenient miniprep, midiprep, 8-well strip, and 96-well plate formats that offer high flexibility with regard to sample volume and sample type.
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NucleoSpin RNA Blood
NucleoSpin RNA Blood / 8 RNA Blood / 96 RNA Blood / RNA Blood Midi
RNA from whole blood
- Direct total blood lysis using a very simple, convenient procedure
Room-temperature protocol: no need for selective erythrocyte lysis at 4°C
- Outstanding RNA yields from up to 1.3 ml of blood
Typical yield: 3–20 μg per ml of whole blood
- Efficient DNA removal
Convenient on-column DNA digestion using high-quality recombinant DNase included with kit
- Compatible with common blood collection tubes and anticoagulants
Suitable for EDTA, citrate, and other blood collection systems
- Available in various formats
Single-tube, 8-well, 96-well, and midiprep formats are available
Higher yields from smaller sample volumes than competitor kits
NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi kits were shown to provide higher yields of high-quality RNA from smaller sample volumes than competitor kits (Figure 2).
Efficient, reliable protocol
The yield of NucleoSpin RNA Blood kits was shown to increase linearly with increased sample volume (Figure 3), indicating the efficiency and reliability of the kit protocol.
Consistent yields and quality from fresh or frozen samples
Fresh and frozen blood samples were used to purify RNA of comparably high yield and quality (Figure 4).
Analysis of RNA purified from aliquots of fresh whole blood using NucleoSpin 8 RNA Blood indicated that the kit consistently yielded high-quality samples (Figure 5).
Effective DNA removal
PCR analysis of RNA isolated manually under vacuum using NucleoSpin 96 RNA Blood indicated that inclusion of the on-column DNase treatment in the kit protocol resulted in a ~64X reduction of gDNA in the eluted RNA (Figure 6).
Direct lysis provides higher yields than selective erythrocyte lysis
Direct lysis using a NucleoSpin RNA Blood kit was shown to provide higher yields of RNA than the selective erythrocyte lysis protocol of a competitor kit (Figure 7).
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