We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to RNA purification
  • RNA purification overview
  • NucleoSpin RNA Plus
  • NucleoSpin RNA Plus XS
  • NucleoSpin RNA
  • NucleoProtect RNA
  • NucleoSpin miRNA
  • NucleoSpin RNA Blood
  • NucleoSpin miRNA Plasma
  • NucleoZOL for RNA isolation
  • High-throughput RNA isolation from FFPE samples
  • NucleoSpin RNA Plant and Fungi
RNA purification kits All RNA purification kits
Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Home › Learning centers › Nucleic acid purification › RNA purification › NucleoSpin RNA Blood

Nucleic acid purification

  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • Plasmid purification
    • NucleoSpin Plasmid Transfection-grade
    • NucleoSnap Plasmid Midi
    • NucleoBond Xtra Midi/Maxi
  • Genomic DNA purification
    • Tissue
      • NucleoSpin Tissue
      • NucleoSpin DNA RapidLyse
      • NucleoSpin Tissue XS
      • NucleoMag DNA Swab
      • NucleoBond HMW DNA
      • NucleoSpin DNA Lipid Tissue
    • FFPE
      • NucleoMag DNA FFPE
      • NucleoSpin 96 DNA FFPE
    • Blood and plasma
      • NucleoMag Blood
      • NucleoSnap cfDNA
      • NucleoSpin cfDNA XS
      • NucleoMag cfDNA
      • NucleoSpin cfDNA Midi
    • Plant
      • NucleoMag 384 Plant
      • NucleoSpin Plant II
    • Other organisms and samples
      • NucleoMag DNA Bacteria
      • NucleoMag DNA Food
      • NucleoSpin DNA Insect
      • NucleoSpin Microbial DNA
  • DNA/RNA cleanup and extraction
    • NucleoSpin Gel and PCR XS product overview
    • NucleoSpin Gel and PCR Clean-up
    • NucleoMag NGS Clean-up and Size Select
    • NucleoSpin RNA Clean-up
  • RNA purification
    • RNA purification overview
    • NucleoSpin RNA Plus
    • NucleoSpin RNA Plus XS
    • NucleoSpin RNA
    • NucleoProtect RNA
    • NucleoSpin miRNA
    • NucleoSpin RNA Blood
    • NucleoSpin miRNA Plasma
    • NucleoZOL for RNA isolation
    • High-throughput RNA isolation from FFPE samples
    • NucleoSpin RNA Plant and Fungi
  • RNA purification kit finder
  • Viral DNA and RNA purification
    • Nucleic acid detection from wastewater
    • NucleoSpin VET
    • NucleoMag VET
    • NucleoMag Virus
    • NucleoMag Pathogen
  • Parallel DNA, RNA & protein
    • NucleoSpin RNA/Protein
    • NucleoSpin TriPrep
  • Automated DNA and RNA purification
    • Application notes, by sample type
      • Plasmid purification
      • DNA cleanup
      • DNA from cells and tissues
      • RNA from cells and tissues
      • DNA from blood and bodily fluids
      • DNA and RNA from plants, fungi, and food
      • Viral RNA and DNA purification
    • Application notes, by platform
      • Eppendorf
      • Tecan
      • Hamilton
      • Thermo Fisher Scientific
      • MASMEC Biomed
  • Accessory selection guides
    • Sample homogenization beads
    • NucleoBond racks selection guide
  • Microbiome
    • NucleoMag DNA Microbiome
    • NucleoSpin Soil
    • NucleoSpin 8/96 Soil
    • NucleoSpin DNA Stool
    • NucleoSpin 96 DNA Stool
    • NucleoMag DNA/RNA Water
    • NucleoSpin eDNA Water
    • NucleoBond RNA Soil
    • NucleoSpin RNA Stool
New products
Need help?
Contact Sales
RNA purification kits All RNA purification kits
Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Tech Note

NucleoSpin RNA Blood

NucleoSpin RNA Blood / 8 RNA Blood / 96 RNA Blood / RNA Blood Midi

RNA from whole blood

  • Direct total blood lysis using a very simple, convenient procedure
    Room-temperature protocol: no need for selective erythrocyte lysis at 4°C
  • Outstanding RNA yields from up to 1.3 ml of blood
    Typical yield: 3–20 μg per ml of whole blood
  • Efficient DNA removal
    Convenient on-column DNA digestion using high-quality recombinant DNase included with kit
  • Compatible with common blood collection tubes and anticoagulants
    Suitable for EDTA, citrate, and other blood collection systems
  • Available in various formats
    Single-tube, 8-well, 96-well, and midiprep formats are available
Protocol overview Application data Product-at-a-glance

Protocol overview  

NucleoSpin RNA Blood kits provide high yields of high-quality total RNA using a simple, direct blood cell lysis procedure which is very effective at room temperature, without the need for an upstream selective erythrocyte lysis at 4°C (Figure 1). These kits are available in convenient miniprep, midiprep, 8-well strip, and 96-well plate formats that offer high flexibility with regard to sample volume and sample type.

The NucleoSpin RNA Blood procedure

Figure 1. The NucleoSpin RNA Blood procedure.

Application data  

Higher yields from smaller sample volumes than competitor kits

NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi kits were shown to provide higher yields of high-quality RNA from smaller sample volumes than competitor kits (Figure 2). 

Higher yields from smaller sample volumes than competitor kits

Figure 2. Higher yields from smaller sample volumes than competitor kits. RNA was isolated from the indicated blood volumes following each manufacturer's protocol. The quality of isolated RNA is comparable. Average RIN numbers (data not shown) were: 7.5 (Q); 7.7 (NucleoSpin RNA Blood); 8.8 (NucleoSpin RNA Blood Midi); 8.9 (Q, PAXgene).

Efficient, reliable protocol

The yield of NucleoSpin RNA Blood kits was shown to increase linearly with increased sample volume (Figure 3), indicating the efficiency and reliability of the kit protocol.

Proportional increase in yield with increasing sample volume

Figure 3. Proportional increase in yield with increasing sample volume. NucleoSpin RNA Blood Midi was used to isolate RNA from 400-µl, 700-µl, and 1,300-μl blood (EDTA) aliquots obtained from three different donors (Blood A–C). Yields increased in proportion to the sample volume used, indicating the high efficiency and reliability of this procedure.

Consistent yields and quality from fresh or frozen samples

Fresh and frozen blood samples were used to purify RNA of comparably high yield and quality (Figure 4).

Consistent RNA yield and quality from fresh or frozen blood samples

Figure 4. Consistent RNA yield and quality from fresh or frozen blood samples. NucleoSpin RNA Blood was used to isolate RNA in triplicate from 400-μl fresh and frozen blood (EDTA) samples under vacuum. Samples originated from six individual blood donors. Frozen sample aliquots were stored at –20°C. RNA yield and quality were determined by spectrophotometric measurement of absorption at 260 nm and 280 nm. The diagrams show the average yield (Panel A) and A260/A280 ratio (Panel B) calculated for each triplicate sample.

High-quality RNA

Analysis of RNA purified from aliquots of fresh whole blood using NucleoSpin 8 RNA Blood indicated that the kit consistently yielded high-quality samples (Figure 5).

High-quality RNA with NucleoSpin 8 RNA Blood

Figure 5. High-quality RNA. RNA was isolated from 400-μl aliquots of fresh whole blood from three individual blood samples using NucleoSpin 8 RNA Blood. Electropherograms were generated using an Agilent Bioanalyzer 2100 with RNA Nano 6000 reagents. The RNA integrity number (RIN) ranged from 8.0 to 8.3.

Effective DNA removal 

PCR analysis of RNA isolated manually under vacuum using NucleoSpin 96 RNA Blood indicated that inclusion of the on-column DNase treatment in the kit protocol resulted in a ~64X reduction of gDNA in the eluted RNA (Figure 6).

Efficient DNA removal

Figure 6. Effective DNA removal. Total RNA was isolated manually under vacuum from 400-μl samples of whole blood according to the standard protocol for NucleoSpin 96 RNA Blood, either with or without the inclusion of the on-column DNase treatment (+DNase or -DNase, respectively). The purified RNA samples were then analyzed by qPCR, along with a negative control sample (NTC) and a dilution series of DNA standards (0.01–100 ng) using the human elongation factor EF-1a as an amplification target. In total, blood samples from six individual donors were processed in this manner (data not shown). RNA samples treated with DNase yielded a Cp value of 31.8 on average, while untreated samples yielded an average Cp value of 25.9, suggesting that inclusion of the on-column DNase treatment in the kit protocol resulted in a ~64X reduction of gDNA in the eluted RNA.

Direct lysis provides higher yields than selective erythrocyte lysis

Direct lysis using a NucleoSpin RNA Blood kit was shown to provide higher yields of RNA than the selective erythrocyte lysis protocol of a competitor kit (Figure 7).

Direct lysis results in higher yields than selective erythrocyte lysis

Figure 7. Direct lysis results in higher yields than selective erythrocyte lysis. RNA was isolated from 400 μl of blood (EDTA) from two different donors (Blood A, B) with the NucleoSpin RNA Blood kit and a kit from Competitor Q (based on selective erythrocyte lysis). Samples were analyzed in duplicates. For both samples, Ct values were lower for NucleoSpin RNA Blood, indicating a higher RNA yield. The isolated RNA was analyzed with LightCycler RT-PCR using a β-actin-specific primer and a 73-nt amplification target.

Product-at-a-glance  

NucleoSpin RNA Blood NucleoSpin RNA Blood Midi NucleoSpin 8/96 RNA Blood
Technology Silica membrane Silica membrane Silica membrane
Format Mini spin columns Midi spin columns 8-well strips/
96-well plates
Starting material <400 µl of whole blood
(fresh or frozen)
400–1,300 µl of whole blood (fresh or frozen) <400 µl of whole blood
(fresh or frozen)
Fragment size >200 nt >200 nt >200 nt
Typical yield 1–8 µg
(400 µl of whole blood)*
4–26 µg
(1,300 µl of whole blood)*
1–8 µg
(400 µl of whole blood)*
A260/A280 1.9–2.1 1.9–2.1 1.9–2.1
Elution volume 40–120 µl 200–400 µl 50–130 µl
Preparation time 55 min/6 preps 75 min/6 preps 60 min/6 strips
100 min/plate
Binding capacity 200 µg 700 µg 100 µg

*RNA yield depends on the leukocyte number in each individual blood sample.

Related products

Cat. # Product Size License Quantity Details
740200.50 NucleoSpin® RNA Blood 50 Preps USD $306.00

NucleoSpin RNA Blood provides a simple and convenient method for purifying RNA from up to 400 µl of fresh or frozen whole blood that has been stabilized using anticoagulants such as EDTA, citrate, or heparin. RNA is isolated via direct total blood lysis without the need for selective erythrocyte lysis at 4 °C, such that the entire procedure is performed at room temperature. Following binding of RNA to NucleoSpin RNA Blood mini spin columns, DNA is efficiently removed via an on-column rDNase digestion. Contaminants and inhibitors are then removed with two different wash buffers, and RNA is eluted with RNase-free water. The highly efficient kit protocol enables processing of 6 samples in less than an hour.

Cat. # 740200.50 includes sufficient quantities of reagents and materials for performing 50 preps.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

The NucleoSpin RNA Blood procedure

The NucleoSpin RNA Blood procedure
The NucleoSpin RNA Blood procedure.

Back

NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits

NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits
NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits. RNA was isolated from the indicated blood volumes following each manufacturer's protocol. The quality of isolated RNA is comparable. Average RIN numbers (data not shown): 7.5 (Q); 7.7 (NucleoSpin RNA Blood); 8.8 (NucleoSpin RNA Blood Midi); 8.9 (Q, PAXgene).

Back

740200.50: NucleoSpin RNA Blood

740200.50: NucleoSpin RNA Blood
740210.20 NucleoSpin® RNA Blood Midi 20 Preps USD $268.00

The NucleoSpin RNA Blood Midi kit enables efficient recovery of high-quality RNA from up to 1.3 ml of whole blood using a simple and convenient procedure that can be performed at room temperature. The kit protocol is compatible with common blood collection tubes and anticoagulants—including EDTA, citrate, and heparin—and enables processing of 6 samples in 75 minutes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

The NucleoSpin RNA Blood procedure

The NucleoSpin RNA Blood procedure
The NucleoSpin RNA Blood procedure.

Back

NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits

NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits
NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits. RNA was isolated from the indicated blood volumes following each manufacturer's protocol. The quality of isolated RNA is comparable. Average RIN numbers (data not shown): 7.5 (Q); 7.7 (NucleoSpin RNA Blood); 8.8 (NucleoSpin RNA Blood Midi); 8.9 (Q, PAXgene).

Back

The NucleoSpin RNA Blood Midi procedure

The NucleoSpin RNA Blood Midi procedure
The NucleoSpin RNA Blood Midi procedure.

Back

740210.20: NucleoSpin RNA Blood Midi

740210.20: NucleoSpin RNA Blood Midi
740225.4 NucleoSpin® 96 RNA Blood 4 x 96 Preps USD $1625.00

The NucleoSpin 96 RNA Blood kit is (4 x 96 preps) is designed for purifying RNA from whole blood on 96-well plates. Samples are lysed in chaotropic salts, which immediately inactivate RNases present in virtually all biological materials and create the binding conditions favoring adsorption of RNA to the silica membrane on the plate. Contaminating DNA, which is also bound to the silica membrane, is removed by the recombinant DNase solution. Samples can be processed using either vacuum or centrifugation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Effective DNA removal

Effective DNA removal

Effective DNA removal. Total RNA was isolated manually under vacuum from 400-µl whole blood samples (EDTA) according to the standard protocol for NucleoSpin 96 RNA Blood, with and without an on-column rDNase treatment. A PCR for human elongation factor EF-1a was performed to determine the amount of residual genomic DNA in the eluted RNA using a LightCycler system and gene-specific primers designed to yield a 469-bp product. The figure shows the corresponding amplification curves of the 0.01 ng–100 ng DNA standards (dark blue lines), the NTC (negative control; green), and replicates of the blood sample 1 (1-1, 1-2), with DNase treatment (+ DNase; red lines) and without DNase treatment (-DNase; light blue lines). In total, blood samples from six individual donors were analyzed by PCR (data not shown). Samples treated with rDNase (+DNase) show an average Cp of 31.8. Samples purified without rDNase (-DNase) treatment show an average Cp of 25.9. Omitting the on-column DNA digestion results in an approximately 64 times higher amount of residual gDNA.

Back

Consistent RNA quality from fresh or frozen blood samples

Consistent RNA quality from fresh or frozen blood samples

Consistent RNA quality from fresh or frozen blood samples. NucleoSpin 96 RNA Blood was used to isolate RNA in triplicate from 400-µl fresh or frozen blood (EDTA) samples obtained from six different donors under vacuum. Frozen sample aliquots had been stored at –20°C. RNA quality was determined by spectrophotometric measurement of absorption at 260 nm and 280 nm.

Back

Consistent RNA yield from fresh or frozen blood samples

Consistent RNA yield from fresh or frozen blood samples

Consistent RNA yield from fresh or frozen blood samples. NucleoSpin 96 RNA Blood was used to isolate RNA in triplicate from 400-µl fresh or frozen blood (EDTA) samples obtained from six different donors under vacuum. Frozen sample aliquots had been stored at –20°C. RNA yield was determined by spectrophotometric measurement of absorption at 260 nm and 280 nm.

Back

740225.4: NucleoSpin 96 RNA Blood

740225.4: NucleoSpin 96 RNA Blood
740225.2 NucleoSpin® 96 RNA Blood 2 x 96 Preps USD $915.00

The NucleoSpin 96 RNA Blood kit (2 x 96 preps) is designed for purifying RNA from whole blood on 96-well plates. Samples are lysed in chaotropic salts, which immediately inactivate RNases present in virtually all biological materials and create the binding conditions favoring adsorption of RNA to the silica membrane on the plate. Contaminating DNA, which is also bound to the silica membrane, is removed by the recombinant DNase solution. Samples can be processed using either vacuum or centrifugation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Effective DNA removal

Effective DNA removal

Effective DNA removal. Total RNA was isolated manually under vacuum from 400-µl whole blood samples (EDTA) according to the standard protocol for NucleoSpin 96 RNA Blood, with and without an on-column rDNase treatment. A PCR for human elongation factor EF-1a was performed to determine the amount of residual genomic DNA in the eluted RNA using a LightCycler system and gene-specific primers designed to yield a 469-bp product. The figure shows the corresponding amplification curves of the 0.01 ng–100 ng DNA standards (dark blue lines), the NTC (negative control; green), and replicates of the blood sample 1 (1-1, 1-2), with DNase treatment (+ DNase; red lines) and without DNase treatment (-DNase; light blue lines). In total, blood samples from six individual donors were analyzed by PCR (data not shown). Samples treated with rDNase (+DNase) show an average Cp of 31.8. Samples purified without rDNase (-DNase) treatment show an average Cp of 25.9. Omitting the on-column DNA digestion results in an approximately 64 times higher amount of residual gDNA.

Back

Consistent RNA quality from fresh or frozen blood samples

Consistent RNA quality from fresh or frozen blood samples

Consistent RNA quality from fresh or frozen blood samples. NucleoSpin 96 RNA Blood was used to isolate RNA in triplicate from 400-µl fresh or frozen blood (EDTA) samples obtained from six different donors under vacuum. Frozen sample aliquots had been stored at –20°C. RNA quality was determined by spectrophotometric measurement of absorption at 260 nm and 280 nm.

Back

Consistent RNA yield from fresh or frozen blood samples

Consistent RNA yield from fresh or frozen blood samples

Consistent RNA yield from fresh or frozen blood samples. NucleoSpin 96 RNA Blood was used to isolate RNA in triplicate from 400-µl fresh or frozen blood (EDTA) samples obtained from six different donors under vacuum. Frozen sample aliquots had been stored at –20°C. RNA yield was determined by spectrophotometric measurement of absorption at 260 nm and 280 nm.

Back

740225.2: NucleoSpin 96 RNA Blood

740225.2: NucleoSpin 96 RNA Blood
740220 NucleoSpin® 8 RNA Blood 12 x 8 Preps USD $570.00

The NucleoSpin 8 RNA Blood kit is designed for purifying RNA from fresh or frozen whole blood in an 8 well-strip format. Samples are lysed in chaotropic salts, which immediately inactivate RNases present in virtually all biological materials and create binding conditions favoring adsorption of RNA to the silica membrane in each well. Contaminating DNA, which is also bound to the silica membrane, is removed by the recombinant DNase solution. Samples can be processed using either vacuum or centrifugation. NucleoSpin 8 RNA Blood typically yields 1–8 µg of total RNA from 400-µl inputs, and enables processing of 48 samples (6 strips) in 1 hour.

Cat. # 740220 includes sufficient quantities of reagents and materials for processing of 96 samples (12 x 8 preps).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

High-quality RNA

High-quality RNA

High-quality RNA. RNA was isolated from 400-µl aliquots of fresh whole blood from three individual blood samples using NucleoSpin 8 RNA Blood. Electropherograms were obtained with an Agilent Bioanalyzer 2100 using RNA Nano 6000 reagents. The RNA integrity number (RIN) ranged from 8.0 to 8.30.

Back

740220: NucleoSpin 8 RNA Blood

740220: NucleoSpin 8 RNA Blood
740220.5 NucleoSpin® 8 RNA Blood 60 x 8 Preps USD $2375.00

The NucleoSpin 8 RNA Blood kit is designed for purifying RNA from fresh or frozen whole blood in an 8 well-strip format. Samples are lysed in chaotropic salts, which immediately inactivate RNases present in virtually all biological materials and create binding conditions favoring adsorption of RNA to the silica membrane in each well. Contaminating DNA, which is also bound to the silica membrane, is removed by the recombinant DNase solution. Samples can be processed using either vacuum or centrifugation. NucleoSpin 8 RNA Blood typically yields 1–8 µg of total RNA from 400-µl inputs, and enables processing of 48 samples (6 strips) in 1 hour.

Cat. # 740220.5 includes sufficient quantities of reagents and materials for processing of 480 samples (60 x 8 preps).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

High-quality RNA

High-quality RNA

High-quality RNA. RNA was isolated from 400-µl aliquots of fresh whole blood from three individual blood samples using NucleoSpin 8 RNA Blood. Electropherograms were obtained with an Agilent Bioanalyzer 2100 using RNA Nano 6000 reagents. The RNA integrity number (RIN) ranged from 8.0 to 8.30.

Back

740220: NucleoSpin 8 RNA Blood

740220: NucleoSpin 8 RNA Blood

Back

740220.5: NucleoSpin 8 RNA Blood

740220.5: NucleoSpin 8 RNA Blood

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2023 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Diagnostic solutions
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Services & Support
  • OEM
  • Instrument services
  • Stem cell services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Capabilities and installations
  • OEM enzyme FAQs
  • Enzyme samples for commerical assay developers
  • OEM process
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • cDNA synthesis
  • Real-time PCR
  • Nucleic acid purification
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • Vaccine development
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us