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NucleoSpin 96 DNA FFPE product page NucleoSpin 96 DNA FFPE
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Home › Learning centers › Nucleic acid purification › Genomic DNA purification › FFPE › NucleoSpin 96 DNA FFPE

Genomic DNA purification

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NucleoSpin 96 DNA FFPE product page NucleoSpin 96 DNA FFPE
automated nucleic acid purification Automate your workflow

Need help automating your nucleic acid purification workflow?

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Product overview

NucleoSpin 96 DNA FFPE

The NucleoSpin 96 DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. Paraffin is first removed from the sample using odorless, xylene-free Paraffin Dissolver, which enables effective lysis in a convenient, two-phase system. Samples are then digested by Proteinase K to solubilize fixed tissues and release DNA into solution. Subsequently, crosslinks are removed via heat incubation in a specially designed buffer, and large amounts of chaotropic salt and ethanol are added to the lysate, providing conditions suitable for binding of DNA to the silica membrane in the NucleoSpin DNA FFPE Binding Plate. The binding process is reversible and specific to nucleic acids. While DNA is kept on the silica membrane, contaminants are removed by washing with two different wash buffers. Pure genomic DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer.

  • Isolation of DNA from FFPE samples in a high-throughput, 96-well plate format
  • Blue-colored, xylene-free Paraffin Dissolver for easy paraffin removal
  • Decrosslinking buffer to eliminate formalin crosslinking
  • High-quality DNA for improved performance in downstream applications
  • Suitable for manual and automated processing under centrifugation or vacuum
Procedure Reliable DNA yield Consistent PCR performance At-a-glance

Procedure  

NucleoSpin 96 DNA FFPE procedure

Figure 1. NucleoSpin 96 DNA FFPE procedure. The protocol can be performed using centrifugation or vacuum processing and can be automated on common liquid-handling platforms.

Reliable DNA yield  

Reliable DNA yield using NucleoSpin 96 DNA FFPE.

Figure 2. Reliable DNA yield with NucleoSpin 96 DNA FFPE. DNA was purified from seven FFPE samples derived from human ileum and stomach and quantified using the Quantifiler Human DNA Quantification Kit. Average DNA yields of 0.3 µg and 1.7 µg were obtained from the ileum and stomach, respectively.

Consistent PCR performance  

Consistent PCR performance with NucleoSpin 96 DNA FFPE

Figure 3. Consistent PCR performance with NucleoSpin 96 DNA FFPE. Genomic DNA was isolated from mouse liver, lung, muscle, and kidney FFPE tissues (8 samples each) with the NucleoSpin 96 DNA FFPE kit. Panel A. Analysis by qPCR. Purified DNA was quantified using Maxima SYBR Green qPCR Master Mix (amplicon size: 100 bp). The similar CT values observed for each sample type demonstrate the consistent performance of the kit in yielding DNA suitable for PCR amplification. Panel B. Analysis by gel electrophoresis. Agarose gel electrophoresis of PCR products from the 8 different samples of mouse liver genomic DNA indicated that DNA quality and yield were comparable for each.

At-a-glance  

Technology Silica membrane
Format 96-well plate
Processing Manual or automated, vacuum or centrifugation
Starting material ≤10 mg tissue
≤15 mg paraffin
Fragment size 50 bp–approx. 5 kb
Typical yield Depends on the amount and quality of the sample
A260/280 Strongly depends on sample quality
Elution volume 100 µl
Preparation time ~60 min/plate (excl. lysis)
Binding capacity 20 µg

Related Products

Cat. # Product Size License Quantity Details
740240.1 NucleoSpin® 96 DNA FFPE 1 x 96 Preps USD $585.00

The NucleoSpin 96 DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples, employing an odorless, xylene-free paraffin removal technology. After the paraffin is dissolved, Proteinase K digestion is used to solubilize the fixed tissue and release DNA into solution, and the samples are heated in an optimized buffer to remove crosslinks. DNA is then bound to the NucleoSpin silica membrane in the presence of ethanol and chaotropic salts, followed by two washing steps and elution of purified DNA under low ionic strength conditions in a slightly alkaline buffer. The kit protocol can be performed using centrifugation or vacuum processing, and can also be automated on common liquid-handling platforms.

Cat. # 740240.1 includes sufficient quantities of reagents and materials for processing of 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin 96 DNA FFPE procedure

NucleoSpin 96 DNA FFPE procedure

NucleoSpin 96 DNA FFPE procedure. The protocol can be performed using centrifugation or vacuum processing, and can be automated on common liquid-handling platforms.

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Reliable DNA yield with NucleoSpin 96 DNA FFPE

Reliable DNA yield with NucleoSpin 96 DNA FFPE

Reliable DNA yield with NucleoSpin 96 DNA FFPE. DNA was purified from seven FFPE samples derived from human ileum and stomach, respectively, and quantified using the Quantifiler Human DNA Quantification Kit. Average DNA yields of 0.3 µg and 1.7 µg were obtained from the ileum and stomach samples, respectively.

Back

Consistent PCR performance with NucleoSpin 96 DNA FFPE

Consistent PCR performance with NucleoSpin 96 DNA FFPE

Consistent PCR performance with NucleoSpin 96 DNA FFPE. Genomic DNA was isolated from mouse liver, lung, muscle, and kidney FFPE tissues (8 samples each) with the NucleoSpin 96 DNA FFPE Kit. Panel A. Analysis by qPCR. Purified DNA was quantified using Maxima SYBR Green qPCR Master Mix (amplicon size: 100 bp). The similar CT values observed for each sample type demonstrate the consistent performance of the kit in yielding DNA suitable for PCR amplification. Panel B. Analysis by gel electrophoresis. Agarose gel electrophoresis of PCR products from the 8 different samples of mouse liver genomic DNA indicated that DNA quality and yield were comparable for each.

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740240.1: NucleoSpin 96 DNA FFPE

740240.1: NucleoSpin 96 DNA FFPE
740240.4 NucleoSpin® 96 DNA FFPE 4 x 96 Preps USD $1840.00

The NucleoSpin 96 DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples, employing an odorless, xylene-free paraffin removal technology. After the paraffin is dissolved, Proteinase K digestion is used to solubilize the fixed tissue and release DNA into solution, and the samples are heated in an optimized buffer to remove crosslinks. DNA is then bound to the NucleoSpin silica membrane in the presence of ethanol and chaotropic salts, followed by two washing steps and elution of purified DNA under low ionic strength conditions in a slightly alkaline buffer. The kit protocol can be performed using centrifugation or vacuum processing, and can also be automated on common liquid-handling platforms.

Cat. # 740240.4 includes sufficient quantities of reagents and materials for processing of 4 x 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

NucleoSpin 96 DNA FFPE procedure

NucleoSpin 96 DNA FFPE procedure

NucleoSpin 96 DNA FFPE procedure. The protocol can be performed using centrifugation or vacuum processing, and can be automated on common liquid-handling platforms.

Back

Reliable DNA yield with NucleoSpin 96 DNA FFPE

Reliable DNA yield with NucleoSpin 96 DNA FFPE

Reliable DNA yield with NucleoSpin 96 DNA FFPE. DNA was purified from seven FFPE samples derived from human ileum and stomach, respectively, and quantified using the Quantifiler Human DNA Quantification Kit. Average DNA yields of 0.3 µg and 1.7 µg were obtained from the ileum and stomach samples, respectively.

Back

Consistent PCR performance with NucleoSpin 96 DNA FFPE

Consistent PCR performance with NucleoSpin 96 DNA FFPE

Consistent PCR performance with NucleoSpin 96 DNA FFPE. Genomic DNA was isolated from mouse liver, lung, muscle, and kidney FFPE tissues (8 samples each) with the NucleoSpin 96 DNA FFPE Kit. Panel A. Analysis by qPCR. Purified DNA was quantified using Maxima SYBR Green qPCR Master Mix (amplicon size: 100 bp). The similar CT values observed for each sample type demonstrate the consistent performance of the kit in yielding DNA suitable for PCR amplification. Panel B. Analysis by gel electrophoresis. Agarose gel electrophoresis of PCR products from the 8 different samples of mouse liver genomic DNA indicated that DNA quality and yield were comparable for each.

Back

740240.4: NucleoSpin 96 DNA FFPE

740240.4: NucleoSpin 96 DNA FFPE
740343.60 Paraffin Dissolver (blue) 60 mL USD $74.00

Paraffin Dissolver (blue) is an odorless, xylene-free reagent that enables efficient sample deparaffinization for purification of DNA or RNA from FFPE tissues. Cat. # 740343.60 consists of a bottle containing 60 ml of Paraffin Dissolver (blue) reagent.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740343.60: Paraffin Dissolver (blue)

740343.60: Paraffin Dissolver (blue)
740923 Buffer BQ1 125 mL USD $57.00

Buffer BQ1 (125 mL) Lysis Buffer BQ1 bottle of 125 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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740923: Buffer BQ1

740923: Buffer BQ1
740921 Buffer B5 Concentrate 20 mL USD $37.00

Buffer B5 Concentrate (25 mL) Wash Buffer B5 Concentrate, for 125 mL Wash Buffer B5 bottle of 25 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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740921: Buffer B5 Concentrate

740921: Buffer B5 Concentrate
740922 Buffer BW 100 mL USD $34.00

Buffer BW (100 mL) Wash Buffer BW bottle of 100 mL

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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740922: Buffer BW

740922: Buffer BW
740506 Proteinase K 100 mg USD $87.00

Proteinase K is a robust endopeptidase that inactivates and destroys proteins without any pronounced cleavage specificity. Cat. # 740506 consists of 100 mg of lyophilized Proteinase K (with an activity of >2.5 U/mg) plus 8 ml of a stabilizing solution (Proteinase Buffer PB).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740506: Proteinase K

740506: Proteinase K
740505 RNase A 100 mg USD $86.00

RNase A (100 mg) lyophilized RNase A pack of 100 mg no dangerous goods according transport regulations

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740505: RNase A

740505: RNase A
740505.50 RNase A 50 mg USD $49.00

RNase A (50 mg) lyophilized RNase A pack of 50 mg no dangerous goods according transport regulations

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740505.50: RNase A

740505.50: RNase A

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740505: RNase A

740505: RNase A
740681 NucleoVac™ 96 Vacuum Manifold Each USD $665.00

NucleoVac 96 Vacuum Manifold vacuum manifold for use of NucleoSpin 8/96 kits under vacuum - manifold base, manifold lid, waste containers, spacer set

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin Tissue purification in less than 1 hour

NucleoSpin Tissue purification in less than 1 hour

NucleoSpin Tissue purification in less than 1 hour. Mouse or rat tail sections, whole tissue, or cultured cells are incubated with lysis buffer and Proteinase K—no further extraction is necessary. After spinning down the lysis mixture, the clear lysates are transferred to the columns and centrifuged to bind the DNA to the column. This is followed by removal of the cellular contaminants with the provided wash buffers, and elution of the purified genomic DNA. No alcohol precipitation is required. The resulting DNA preparation is suitable for PCR, Southern blotting, or any enzymatic reaction. Panel A. NucleoSpin 8 Tissue Kit. Panel B. NucleoSpin 96 Tissue Kit.

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Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections.

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740681: NucleoVac 96 Vacuum Manifold

740681: NucleoVac 96 Vacuum Manifold
740641 NucleoVac™ Vacuum Regulator Each USD $194.00

NucleoVac 96 Vacuum Regulator for use with NucleoVac 96 Vacuum Manifold, for controlling of vacuum

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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740641: NucleoVac Vacuum Regulator

740641: NucleoVac Vacuum Regulator
740676 Self-adhering PE Foil 50 Each USD $83.00

Self-adhering PE Foil (50) adhesive tape foils for sealing 96-well elution plates for air-tight storage of DNA / RNA pack of 50

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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740676: Self-adhering PE Foil

740676: Self-adhering PE Foil


Genomic DNA purification articles

NucleoSpin Tissue

Reliably obtain high-quality genomic DNA from a wide array of sample types.

NucleoSpin DNA RapidLyse

Speed up your workflow with a unique lysis chemistry that efficiently releases gDNA from cells, tissues, and organs.

NucleoSpin Tissue XS

Get highly concentrated DNA from small inputs, such as biopsy materials, forensic samples, or FACS-sorted cells.

NucleoSnap DNA Plasma

Obtain high-quality cell-free DNA from larger sample volumes.

NucleoSpin Plasma XS

Capture circulating DNA from low inputs of plasma or serum.

NucleoMag DNA Plasma

Isolate cell-free DNA from up to 10 ml of plasma using scalable magnetic-bead technology.

NucleoMag Pathogen

Efficiently isolate viral and bacterial nucleic acids from a wide array of inputs.

NucleoMag Virus

Automate your workflow for high-throughput isolation of viral nucleic acids from serum or plasma.

NucleoMag Blood

Isolate genomic DNA from whole blood using scalable magnetic-bead technology.

NucleoBond HMW DNA

The NucleoBond HMW DNA kit enables isolation of high-quality genomic DNA up to 200 kb in size for long read-sequencing applications.

NucleoMag DNA FFPE

Purify DNA from FFPE tissue samples using paramagnetic beads.

NucleoSpin 96 DNA FFPE

High-throughput purification of DNA from FFPE samples in a 96-well format.

NucleoMag DNA Swab

The NucleoMag DNA Swab kit enables high-throughput isolation of genomic DNA from diverse swab samples.

NucleoMag VET

Efficiently isolate viral and bacterial nucleic acids from common veterinary samples.

NucleoMag 384 Plant

Scale up your plant DNA extraction workflow with parallel processing of 384 samples.

NucleoSpin DNA Plasma Midi

Avoid tedious centrifugation steps and process up to 24 samples in parallel.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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