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Magnetic-bead-based isolation of viral RNA/DNA and bacterial DNA from veterinary samples
The NucleoMag VET kit is designed for the isolation of viral RNA/DNA and bacterial DNA from common veterinary samples such as whole blood, serum/plasma, feces, ear notches, or swabs using manual or automated processing. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads. Sample lysis is achieved by incubation with a lysis buffer containing chaotropic ions and supplemented by Proteinase K digestion. Nucleic acids are bound to paramagnetic NucleoMag B-Beads in the presence of Binding Buffer VEB. After magnetic separation, the paramagnetic beads are subjected to successive wash steps to remove contaminants and salts, and highly pure RNA/DNA are eluted in low-salt Elution Buffer VEL or water.
One kit for common veterinary samples
High sensitivity, even with low viral titers
Robust, one-tube procedure minimizes risk of cross-contamination
Kit format allows for manual or automated processing on common liquid handling platforms
*For all sample types, start with a maxiumum of 200 µl liquid/homogenized sample.
The NucleoMag family of products is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA are eluted from the beads using elution buffer.
RNA and DNA isolation from pathogens
The NucleoMag Pathogen kit is designed for the rapid manual and automated small-scale isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes.