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  • NucleoSpin RNA/Protein
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Isolate RNA, DNA, and protein in separate fractions without splitting the sample prior to extraction. Parallel preps: RNA/DNA/protein
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Isolate RNA, DNA, and protein in separate fractions without splitting the sample prior to extraction. Parallel preps: RNA/DNA/protein
Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Tech Note

NucleoSpin TriPrep: parallel isolation of RNA, DNA, and protein from one sample

  • Convenient one-column preparation of RNA, DNA, and protein from diverse sample types
  • High-quality RNA and DNA, ready to use for downstream applications
  • High protein yield independent of protein size, localization, modification, etc.
  • Complete kit includes NucleoSpin Filters (shredders) for optimal lysis, rDNase for on-column DNA digestion, and Protein Solving Buffer for dissolving all types of proteins

NucleoSpin TriPrep allows you to isolate DNA, RNA, and protein without splitting the sample prior to extraction. The fractions are obtained from the exact same sample, and not from three similar portions of one sample. This is especially valuable for unique, small, and precious samples.

Product summary Diverse sample types Protocol schematic

Product summary  

Technology Silica membrane
Starting material <5 x 106 cultured cells
<30 mg human/animal tissue
<100 mg plant tissue
Typical yield DNA
RNA
Protein
<6 µg 
<70 µg
<1,200 µg
Typical A260/280 DNA
RNA
1.7–1.9
1.9–2.1
Preparation time RNA/DNA/protein
RNA/DNA
Protein
~60–75 min
~45 min
~35 min

Diverse sample types  

RNA/DNA/protein extraction from a wide variety of starting materials

RNA, DNA, and protein were isolated in parallel from the same source. The range of starting materials includes tissues, cells, plant materials, yeast, and bacteria.

Lane Starting material Sample amount
2 Human cells (HeLa) 106 cells
3 Mouse liver 3 mg
4 Fish (Zebrafish) 30 mg
5 Plant root (garden cress) 100 mg
6 Plant leaves (garden cress) 100 mg
7 Yeast 108 cells
8 Bacteria 109 cells

Total DNA

Figure 1. Total DNA fractions, 1% TAE agarose gel electrophoresis*. High-quality DNA was eluted in 100 µl DNA Elute following the NucleoSpin TriPrep procedure. The DNA is of high molecular weight and purity. A260/A280 ratios are in the range of 1.81–1.94.

Total RNA

The isolated RNA is of high integrity. RIN (RNA integrity number) was measured for mammalian samples. RIN of RNA isolated from HeLa cells was 9.5, RIN of RNA from mouse liver sample was 9.1.

Figure 2. Total RNA fractions analyzed on an Agilent 2100 Bioanalyzer/RNA 6000 Nano Kit*. The isolated RNA is of high integrity. RIN (RNA integrity number) was measured for mammalian samples. RIN of RNA isolated from HeLa cells was 9.5, RIN of RNA from mouse liver sample was 9.1.

Total Protein

Figure 3. Total protein fractions, SDS-PAGE analysis*. Isolated protein is dissolved in Protein Solving Buffer, provided with the kit. The protein is ready-to-use for SDS-PAGE analysis, as well as for Western blotting.  

* Figures are compiled from different gels and runs, aligned to the corresponding length markers.

Protocol schematic  

Samples are lysed by incubation in a buffer containing chaotropic salts. This will not only destroy the cell structure of the sample but simultaneously inactivate enzymes such as RNases, phosphatases, and proteases, and prevent degradation of nucleic acids and proteins. At the same time, the buffer creates appropriate binding conditions for RNA and DNA.

Nucleic acids and protein from the sample can be separated by simply centrifuging the lysate through the NucleoSpin TriPrep spin column: RNA and DNA are bound to the silica membrane while the protein is recovered in the column flowthrough.

The nucleic acids are separated by sequential elution steps. First the DNA is eluted with DNA Elute, a low ionic strength buffer, while RNA is still bound to the column. RNA is isolated, following wash steps, on-column DNA digestion (to remove residual DNA), and elution.

Proteins in the flowthrough are precipitated by a special buffer (Protein Precipitator), pelleted by centrifugation, washed, and dissolved in Protein Solving Buffer.

Isolated RNA and DNA are of high quality and directly suitable for all common downstream applications. Isolated protein is immediately suitable for quantification, SDS-PAGE, and Western blot analysis.

Figure 4. Outline of the NucleoSpin TriPrep workflow.

Related Products

Cat. # Product Size License Quantity Details
740966.50 NucleoSpin® TriPrep 50 Preps USD $466.00

NucleoSpin TriPrep 50 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Isolation of high-quality DNA with with the NucleoSpin TriPrep kit

Isolation of high-quality DNA with with the NucleoSpin TriPrep kit
Isolation of high-quality DNA with with the NucleoSpin TriPrep kit.* Total DNA was eluted in 100 µl DNA Elute buffer (provided with the kit) using the NucleoSpin TriPrep procedure, and fractions were analyzed using 1% TAE agarose gel electrophoresis. The DNA is of high molecular weight and purity. A260/A280 ratios are in the range of 1.81–1.94. The starting material used to isolate the DNA shown in each lane is as follows: Lane 1: DNA Ladder (Lambda/HindIII; Fermentas). Lane 2: Human cells (HeLa), 106 cells. Lane 3: Mouse liver, 3 mg. Lane 4: Fish (Zebrafish), 30 mg. Lane 5: Plant root (garden cress), 100 mg. Lane 6: Plant leaf (garden cress), 100 mg. Lane 7: Yeast, 108 cells. Lane 8: Bacteria, 109 cells.

* Figures are compiled from different gels and runs, aligned to the corresponding length markers.

.

Back

The NucleoSpin TriPrep procedure

The NucleoSpin TriPrep procedure
The NucleoSpin TriPrep procedure.

Back

Isolation of total protein with the NucleoSpin TriPrep kit

Isolation of total protein with the NucleoSpin TriPrep kit
Isolation of total protein with the NucleoSpin TriPrep kit.* Total protein was was isolated using the NucleoSpin TriPrep procedure, dissolved in Protein Solving Buffer (provided with the kit), and analyzed using SDS-PAGE. The isolated protein is ready to use for Western blotting, as well as SDS-PAGE. The starting material used to isolate the DNA shown in each lane is as follows: Lane 1: DNA Ladder (Lambda/HindIII; Fermentas). Lane 2: Human cells (HeLa),106 cells. Lane 3: Mouse liver, 3 mg. Lane 4: Fish (Zebrafish), 30 mg. Lane 5: Plant root (garden cress), 100 mg. Lane 6: Plant leaf (garden cress), 100 mg. Lane 7: Yeast, 108 cells. Lane 8: Bacteria, 109 cells.

* Figures are compiled from different gels and runs, aligned to the corresponding length markers.

.

Back

Isolation of high-integrity RNA with the NucleoSpin TriPrep kit

Isolation of high-integrity RNA with the NucleoSpin TriPrep kit
Isolation of high-integrity RNA with the NucleoSpin TriPrep kit.* Total RNA was isolated using the NucleoSpin TriPrep procedure and analyzed with an Agilent 2100 Bioanalyzer/RNA 6000 Nano Kit. The RIN (RNA integrity number) was measured for mammalian samples. The RIN of RNA isolated from HeLa cells was 9.5, and the RIN of RNA from the mouse liver sample was 9.1. The starting material used to isolate the DNA shown in each lane is as follows: Lane 1: DNA Ladder (Lambda/HindIII; Fermentas). Lane 2: Human cells (HeLa), 106 cells. Lane 3: Mouse liver, 3 mg. Lane 4: Fish (Zebrafish), 30 mg. Lane 5: Plant root (garden cress), 100 mg. Lane 6: Plant leaf (garden cress), 100 mg. Lane 7: Yeast, 108 cells. Lane 8: Bacteria, 109 cells.

* Figures are compiled from different gels and runs, aligned to the corresponding length markers.

.

Back

740966.50: NucleoSpin TriPrep

740966.50: NucleoSpin TriPrep
740966.250 NucleoSpin® TriPrep 250 Preps USD $2080.00

NucleoSpin TriPrep 250 preps for the parallel purification of RNA, DNA, and protein from one sample - NucleoSpin TriPrep Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Isolation of high-quality DNA with with the NucleoSpin TriPrep kit

Isolation of high-quality DNA with with the NucleoSpin TriPrep kit
Isolation of high-quality DNA with with the NucleoSpin TriPrep kit.* Total DNA was eluted in 100 µl DNA Elute buffer (provided with the kit) using the NucleoSpin TriPrep procedure, and fractions were analyzed using 1% TAE agarose gel electrophoresis. The DNA is of high molecular weight and purity. A260/A280 ratios are in the range of 1.81–1.94. The starting material used to isolate the DNA shown in each lane is as follows: Lane 1: DNA Ladder (Lambda/HindIII; Fermentas). Lane 2: Human cells (HeLa), 106 cells. Lane 3: Mouse liver, 3 mg. Lane 4: Fish (Zebrafish), 30 mg. Lane 5: Plant root (garden cress), 100 mg. Lane 6: Plant leaf (garden cress), 100 mg. Lane 7: Yeast, 108 cells. Lane 8: Bacteria, 109 cells.

* Figures are compiled from different gels and runs, aligned to the corresponding length markers.

.

Back

The NucleoSpin TriPrep procedure

The NucleoSpin TriPrep procedure
The NucleoSpin TriPrep procedure.

Back

Isolation of total protein with the NucleoSpin TriPrep kit

Isolation of total protein with the NucleoSpin TriPrep kit
Isolation of total protein with the NucleoSpin TriPrep kit.* Total protein was was isolated using the NucleoSpin TriPrep procedure, dissolved in Protein Solving Buffer (provided with the kit), and analyzed using SDS-PAGE. The isolated protein is ready to use for Western blotting, as well as SDS-PAGE. The starting material used to isolate the DNA shown in each lane is as follows: Lane 1: DNA Ladder (Lambda/HindIII; Fermentas). Lane 2: Human cells (HeLa),106 cells. Lane 3: Mouse liver, 3 mg. Lane 4: Fish (Zebrafish), 30 mg. Lane 5: Plant root (garden cress), 100 mg. Lane 6: Plant leaf (garden cress), 100 mg. Lane 7: Yeast, 108 cells. Lane 8: Bacteria, 109 cells.

* Figures are compiled from different gels and runs, aligned to the corresponding length markers.

.

Back

Isolation of high-integrity RNA with the NucleoSpin TriPrep kit

Isolation of high-integrity RNA with the NucleoSpin TriPrep kit
Isolation of high-integrity RNA with the NucleoSpin TriPrep kit.* Total RNA was isolated using the NucleoSpin TriPrep procedure and analyzed with an Agilent 2100 Bioanalyzer/RNA 6000 Nano Kit. The RIN (RNA integrity number) was measured for mammalian samples. The RIN of RNA isolated from HeLa cells was 9.5, and the RIN of RNA from the mouse liver sample was 9.1. The starting material used to isolate the DNA shown in each lane is as follows: Lane 1: DNA Ladder (Lambda/HindIII; Fermentas). Lane 2: Human cells (HeLa), 106 cells. Lane 3: Mouse liver, 3 mg. Lane 4: Fish (Zebrafish), 30 mg. Lane 5: Plant root (garden cress), 100 mg. Lane 6: Plant leaf (garden cress), 100 mg. Lane 7: Yeast, 108 cells. Lane 8: Bacteria, 109 cells.

* Figures are compiled from different gels and runs, aligned to the corresponding length markers.

.

Back

740966.250: NucleoSpin TriPrep

740966.250: NucleoSpin TriPrep

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