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Home › Learning centers › Nucleic acid purification › Microbiome › NucleoSpin eDNA Water

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Product overview

Rapid purification of environmental DNA from diverse water samples

The NucleoSpin eDNA Water kit enables rapid and reliable purification of environmental DNA (eDNA) from diverse water samples. The kit incorporates silica-matrix and silica-membrane technologies to allow for isolation of high-quality DNA from filters in about an hour, avoiding the long, overnight incubation steps typical of eDNA workflows. Columns included in the kit are treated with ethylene oxide in order to minimize the risk of DNA contamination, and the protocol is compatible with a variety of filters and filtration systems, including conventional bottletop round filters (e.g., 45 mm) as well as cartridge filters (e.g., Sterivex). In addition, an alternative protocol that circumvents filtration by enabling direct eDNA precipitation is provided. As demonstrated below, these properties make NucleoSpin eDNA Water an ideal solution for analyses of both freshwater and saltwater ecosystems with varying levels of organic and inorganic matter.

Key features:

  • Fastest workflow among eDNA kits currently on the market—pure eDNA in hours instead of days
  • Ethylene oxide-treated XS silica membrane columns—minimized risk of DNA contamination
  • Compatible with diverse types of water, filtration systems, and laboratory setups
At-a-glance Procedure Compatibility with different filters and filtration systems Competitor comparison Application data

At-a-glance  

Technology Silica membrane and silica matrix
Starting material Filter, water
Format XS spin columns
Handling Centrifugation (manual)
Fragment size 100 bp–approximately 50 kb
Elution volume 100 µl
Preparation time <70 min (excluding water filtration)

Procedure  

Sample preparation workflow for NucleoSpin eDNA Water

Sample preparation workflow for NucleoSpin eDNA Water. Samples can be obtained either by filtration with round filters or filter cartridges (e.g., Sterivex). DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from <40 ml of unfiltered water (using Buffer PREC, Cat. # 740568). NucleoTrap Suspension beads efficiently bind the DNA while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification steps take less than 70 min, while total preparation times vary depending on the sampling and/or filtration setup.

Compatibility with different filters and filtration systems  

Consistent performance of NucleoSpin eDNA Water with diverse round filters

Consistent performance with diverse round filters. 500-ml samples of creek water were filtered through the following different types of round filters, which were then processed using NucleoSpin eDNA Water: regenerated cellulose (RC), cellulose nitrate (NC), cellulose acetate (CA), polyester (PE), polycarbonate (PC), mixed cellulose ester (CM), and paper. As demonstrated by the resulting Ct values obtained in a downstream qPCR analysis, performance of DNA isolation was similar for each of the filter types and comparable to the performance observed with the recommended ethylene oxide-treated glass fiber filter (GF), which provides the best flow rate and lowest risk of clogging.

Compatibility of NucleoSpin eDNA Water with different water filtration systems

Compatibility with different water filtration systems. Samples of creek water were filtered through a glass fiber filter (GF) as well as several filtration systems commonly used in conjunction with eDNA purification. The NucleoSpin eDNA Water kit was then used to purify eDNA from each filter unit, and eluates were analyzed by qPCR for metazoan DNA. Purification from each filtration system yielded comparable results, as demonstrated by the consistent Ct values.

Competitor comparison  

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits

Superior downstream performance as compared to competitor kits. eDNA was purified from running water (creek) and static water (pond) using the NucleoSpin eDNA Water kit in parallel with two competitor kits (“BT” and “PW”) and analyzed by qPCR for metazoan DNA. As demonstrated by the lower Ct values, NucleoSpin eDNA Water provided higher sensitivity than the competitors for both sample types.

Application data  

Seasonal traceability of European toad in a freshwater pond

Seasonal traceability of European toad in a freshwater pond. In Germany, European toads become active after hibernation—usually in March—and typically start spawning in April. The presence of toads and/or spawn and tadpoles can be monitored by isolation of eDNA and subsequent PCR analysis. The NucleoSpin eDNA Water kit was used in combination with NucleoSpin Filter Midi to isolate DNA from a freshwater pond at the indicated timepoints, and PCR was performed to detect toad-specific or general eukaryotic DNA using corresponding primer pairs.

Related products

Cat. # Product Size License Quantity Details
740402.10 NucleoSpin® eDNA Water 10 Preps USD $142.00

The NucleoSpin eDNA Water kit enables rapid purification of environmental DNA (eDNA) from diverse water samples with reliable results and minimal risk of DNA contamination. The combination of silica matrix and silica membrane technologies allows for efficient isolation of high-quality DNA in about an hour, providing a convenient alternative to the overnight incubation steps typical of eDNA workflows. The XS columns provided with the kit are treated with ethylene oxide in order to minimize the risk of DNA contamination. NucleoSpin eDNA Water is compatible with a variety of filters and filtration systems, such as conventional bottle top filters (e.g., 45 mm) as well as cartridge filters (e.g., Sterivex). Further, an alternative protocol for direct eDNA precipitation from unfiltered water is provided, enabling isolation of free DNA. These properties make NucleoSpin eDNA Water ideal for studies of freshwater and saltwater ecosystems with varying levels of organic and inorganic matter.

Cat. # 740402.10 includes reagents and materials for processing 10 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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740402.10: NucleoSpin eDNA Water

740402.10: NucleoSpin eDNA Water

Back

Compatibility of NucleoSpin eDNA Water with different water filtration systems

Compatibility of NucleoSpin eDNA Water with different water filtration systems

Compatibility of NucleoSpin eDNA Water with different water filtration systems. Samples of creek water were filtered through a glass fiber filter (GF) as well as several filtration systems commonly used in conjunction with eDNA purification. The NucleoSpin eDNA Water kit was then used to purify eDNA from each filter unit, and eluates were analyzed by qPCR for metazoan DNA. Purification from each filtration system yielded comparable results, as demonstrated by the consistent Ct values.

Back

Consistent performance of NucleoSpin eDNA Water with diverse round filters

Consistent performance of NucleoSpin eDNA Water with diverse round filters

Consistent performance of NucleoSpin eDNA Water with diverse round filters. 500-ml samples of creek water were filtered through the following different types of round filters, which were then processed using NucleoSpin eDNA Water: regenerated cellulose (RC), cellulose nitrate (NC), cellulose acetate (CA), polyester (PE), polycarbonate (PC), mixed cellulose ester (CM) and paper. As demonstrated by the resulting Ct values obtained in a downstream qPCR analysis, performance of DNA isolation was similar for each of the filter types and comparable to the performance observed with the recommended ethylene oxide-treated glass fiber filter (GF), which provides the best flow rate and lowest risk of clogging.

Back

Sample preparation workflow for NucleoSpin eDNA Water

Sample preparation workflow for NucleoSpin eDNA Water

Sample preparation workflow for NucleoSpin eDNA Water. Samples can be obtained either by filtration with round filters or filter cartridges (e.g., Sterivex). DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from <40 ml of unfiltered water (using Buffer PREC, Cat. # 740568). NucleoTrap Suspension beads efficiently bind the DNA while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification steps take less than 70 min, while total preparation times vary depending on the sampling and/or filtration setup.

Back

Seasonal traceability of European toad in a freshwater pond

Seasonal traceability of European toad in a freshwater pond

Seasonal traceability of European toad in a freshwater pond. In Germany, European toads become active after hibernation—usually in March—and typically start spawning in April. The presence of toads and/or spawn and tadpoles can be monitored by isolation of eDNA and subsequent PCR analysis. The NucleoSpin eDNA Water kit was used in combination with NucleoSpin Filter Midi to isolate DNA from a freshwater pond at the indicated timepoints, and PCR was performed to detect toad-specific or general eukaryotic DNA using corresponding primer pairs.

Back

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits. eDNA was purified from running water (creek) and static water (pond) using the NucleoSpin eDNA Water kit in parallel with two competitor kits ("BT" and "PW") and analyzed by qPCR for metazoan DNA. As demonstrated by the lower Ct values, NucleoSpin eDNA Water provided higher sensitivity than the competitors.

740402.50 NucleoSpin® eDNA Water 50 Preps USD $555.00

The NucleoSpin eDNA Water kit enables rapid purification of environmental DNA (eDNA) from diverse water samples with reliable results and minimal risk of DNA contamination. The combination of silica matrix and silica membrane technologies allows for efficient isolation of high-quality DNA in about an hour, providing a convenient alternative to the overnight incubation steps typical of eDNA workflows. The XS columns provided with the kit are treated with ethylene oxide in order to minimize the risk of DNA contamination. NucleoSpin eDNA Water is compatible with a variety of filters and filtration systems, such as conventional bottle top filters (e.g., 45 mm) as well as cartridge filters (e.g., Sterivex). Further, an alternative protocol for direct eDNA precipitation from unfiltered water is provided, enabling isolation of free DNA. These properties make NucleoSpin eDNA Water ideal for studies of freshwater and saltwater ecosystems with varying levels of organic and inorganic matter.

Cat. # 740402.50 includes reagents and materials for processing 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

740402.50: NucleoSpin eDNA Water

740402.50: NucleoSpin eDNA Water

Back

Compatibility of NucleoSpin eDNA Water with different water filtration systems

Compatibility of NucleoSpin eDNA Water with different water filtration systems

Compatibility of NucleoSpin eDNA Water with different water filtration systems. Samples of creek water were filtered through a glass fiber filter (GF) as well as several filtration systems commonly used in conjunction with eDNA purification. The NucleoSpin eDNA Water kit was then used to purify eDNA from each filter unit, and eluates were analyzed by qPCR for metazoan DNA. Purification from each filtration system yielded comparable results, as demonstrated by the consistent Ct values.

Back

Consistent performance of NucleoSpin eDNA Water with diverse round filters

Consistent performance of NucleoSpin eDNA Water with diverse round filters

Consistent performance of NucleoSpin eDNA Water with diverse round filters. 500-ml samples of creek water were filtered through the following different types of round filters, which were then processed using NucleoSpin eDNA Water: regenerated cellulose (RC), cellulose nitrate (NC), cellulose acetate (CA), polyester (PE), polycarbonate (PC), mixed cellulose ester (CM) and paper. As demonstrated by the resulting Ct values obtained in a downstream qPCR analysis, performance of DNA isolation was similar for each of the filter types and comparable to the performance observed with the recommended ethylene oxide-treated glass fiber filter (GF), which provides the best flow rate and lowest risk of clogging.

Back

Sample preparation workflow for NucleoSpin eDNA Water

Sample preparation workflow for NucleoSpin eDNA Water

Sample preparation workflow for NucleoSpin eDNA Water. Samples can be obtained either by filtration with round filters or filter cartridges (e.g., Sterivex). DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from <40 ml of unfiltered water (using Buffer PREC, Cat. # 740568). NucleoTrap Suspension beads efficiently bind the DNA while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification steps take less than 70 min, while total preparation times vary depending on the sampling and/or filtration setup.

Back

Seasonal traceability of European toad in a freshwater pond

Seasonal traceability of European toad in a freshwater pond

Seasonal traceability of European toad in a freshwater pond. In Germany, European toads become active after hibernation—usually in March—and typically start spawning in April. The presence of toads and/or spawn and tadpoles can be monitored by isolation of eDNA and subsequent PCR analysis. The NucleoSpin eDNA Water kit was used in combination with NucleoSpin Filter Midi to isolate DNA from a freshwater pond at the indicated timepoints, and PCR was performed to detect toad-specific or general eukaryotic DNA using corresponding primer pairs.

Back

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits. eDNA was purified from running water (creek) and static water (pond) using the NucleoSpin eDNA Water kit in parallel with two competitor kits ("BT" and "PW") and analyzed by qPCR for metazoan DNA. As demonstrated by the lower Ct values, NucleoSpin eDNA Water provided higher sensitivity than the competitors.

740568 Buffer PREC 50 mL USD $47.00

Buffer PREC is a buffer for direct precipitation of eDNA from water, and is intended for use with the NucleoSpin eDNA Water kit. Cat. # 740568 consists of a 50-ml bottle of Buffer PREC. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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740568: Buffer PREC

740568: Buffer PREC
740459 MN Bead Tube Holder 5 mL Each USD $47.00

Blue rubber foam adapter designed for processing 5-ml MN bead tubes with a vortex apparatus (e.g., Vortex Genie 2). MN bead tubes are plastic tubes containing different types of beads. They are designed for the homogenization of hard-to-lyse biological samples prior to purification of nucleic acids.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data

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740459: MN Bead Tube Holder 5 mL

740459: MN Bead Tube Holder 5 mL
740408.10 NucleoSpin® Inhibitor Removal 10 Preps USD $63.00

The NucleoSpin Inhibitor Removal kit is designed for fast and efficient cleanup of pre-purified DNA samples containing substances such as humic acids, heme, polyphenols, or tannins that inhibit PCR amplification. The presence of such inhibitors may be due to insufficient purification procedures or processing of challenging sample types such as soil, blood, fruit, processed food, etc. The kit employs silica membrane technology and a simple mini-spin protocol that enables processing of 6 samples in 15 minutes, with typical recoveries >75%.

Cat. # 740408.10 includes reagents and materials for processing 10 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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Higher purity with NucleoSpin Inhibitor Removal

Higher purity with NucleoSpin Inhibitor Removal

Higher purity with NucleoSpin Inhibitor Removal. Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q). For each sample type, cleanup with NucleoSpin Inhibitor Removal was associated with higher A260/280 and A260/230 ratios as compared to the competitors.

Back

Superior DNA recovery with NucleoSpin Inhibitor Removal

Superior DNA recovery with NucleoSpin Inhibitor Removal

Superior DNA recovery with NucleoSpin Inhibitor Removal. Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q). As shown, DNA recovery was higher with NucleoSpin Inhibitor Removal as compared to the competitors.

Back

Efficient DNA amplification following cleanup with NucleoSpin Inhibitor Removal

Efficient DNA amplification following cleanup with NucleoSpin Inhibitor Removal

Efficient DNA amplification following cleanup with NucleoSpin Inhibitor Removal. Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q), and then subjected to PCR amplification followed by agarose gel electrophoresis. In contrast with untreated samples, and those processed with the competitor kits which failed to yield PCR products, samples processed using NucleoSpin Inhibitor Removal could be reliably amplified.

Back

740408.10: NucleoSpin Inhibitor Removal

740408.10: NucleoSpin Inhibitor Removal
740408.50 NucleoSpin® Inhibitor Removal 50 Preps USD $195.00

The NucleoSpin Inhibitor Removal kit is designed for fast and efficient cleanup of pre-purified DNA samples containing substances such as humic acids, heme, polyphenols, or tannins that inhibit PCR amplification. The presence of such inhibitors may be due to insufficient purification procedures or processing of challenging sample types such as soil, blood, fruit, processed food, etc. The kit employs silica membrane technology and a simple mini-spin protocol that enables processing of 6 samples in 15 minutes, with typical recoveries >75%.

Cat. # 740408.50 includes reagents and materials for processing 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

Higher purity with NucleoSpin Inhibitor Removal

Higher purity with NucleoSpin Inhibitor Removal

Higher purity with NucleoSpin Inhibitor Removal. Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q). For each sample type, cleanup with NucleoSpin Inhibitor Removal was associated with higher A260/280 and A260/230 ratios as compared to the competitors.

Back

Superior DNA recovery with NucleoSpin Inhibitor Removal

Superior DNA recovery with NucleoSpin Inhibitor Removal

Superior DNA recovery with NucleoSpin Inhibitor Removal. Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q). As shown, DNA recovery was higher with NucleoSpin Inhibitor Removal as compared to the competitors.

Back

Efficient DNA amplification following cleanup with NucleoSpin Inhibitor Removal

Efficient DNA amplification following cleanup with NucleoSpin Inhibitor Removal

Efficient DNA amplification following cleanup with NucleoSpin Inhibitor Removal. Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q), and then subjected to PCR amplification followed by agarose gel electrophoresis. In contrast with untreated samples, and those processed with the competitor kits which failed to yield PCR products, samples processed using NucleoSpin Inhibitor Removal could be reliably amplified.

Back

740408.50: NucleoSpin Inhibitor Removal

740408.50: NucleoSpin Inhibitor Removal

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That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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