Fast and reliable isolation of environmental DNA—NucleoSpin eDNA Water
Analysis of environmental DNA (eDNA) can provide a deeper understanding of freshwater and saltwater ecosystems, but common methods for eDNA isolation can be lengthy and laborious, often involving overnight incubation steps. The NucleoSpin eDNA Water kit is designed to streamline eDNA isolation workflows by providing a versatile solution that can be applied to filtered or unfiltered samples, featuring a protocol that can be completed in a little over one hour.
Analysis of environmental DNA (eDNA) can provide a deeper understanding of freshwater and saltwater ecosystems, but common methods for eDNA isolation can be lengthy and laborious, often involving overnight incubation steps. The NucleoSpin eDNA Water kit is designed to streamline eDNA isolation workflows by providing a versatile solution that can be applied to filtered or unfiltered samples, featuring a protocol that can be completed in a little over one hour.
The NucleoSpin eDNA Water workflow has been optimized for speed and simplicity without compromising the quality and purity of isolated DNA. Samples can be obtained by either filtration with round filters or filter cartridges. DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from <40 ml of unfiltered water (protocol included; requires additional buffer PREC). NucleoTrap Bead suspension efficiently binds the DNA, while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification workflow takes less than 70 min, while the full preparation time depends on the sampling and/or filtration setup.
Overview
- Fast workflow—pure eDNA in hours instead of days
- Ethylene oxide-treated XS columns—minimal risk of DNA contamination
- Compatible with diverse types of water, filtration systems, and laboratory setups
More Information
| Technology | Silica membrane and silica matrix |
| Starting material | Filter, water |
| Format | XS spin columns |
| Handling | Centrifugation (manual) |
| Fragment size | 100 bp–approximately 50 kb |
| Elution volume | 100 µl |
| Preparation time | <70 min (excluding water filtration) |
Applications
- Purification of environmental DNA (eDNA) from filters/filter cartridges or unpurified water
- Typical downstream applications: qPCR, NGS
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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