NucleoMag cfDNA
The NucleoMag cfDNA kit is designed for rapid manual or automated isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables efficient recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. cfDNA can be eluted in 50–200 µl of buffer and is ready for applications such as qPCR, ddPCR, NGS, etc.
The NucleoMag cfDNA kit is designed for rapid manual or automated isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables efficient recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. cfDNA can be eluted in 50–200 µl of buffer and is ready for applications such as qPCR, ddPCR, NGS, etc.
The protocol provided in the NucleoMag cfDNA user manual is intended for processing 2-ml inputs of blood plasma, but kit reagents are provided in sufficient quantities for processing input volumes ranging from 1–10 ml. In comparison with a competitor kit developed for cfDNA purification from plasma, NucleoMag cfDNA was shown to provide higher, more consistent yields from challenging samples containing low amounts of cfDNA.
Overview
- Consistent cfDNA recovery from 1–10 ml plasma samples
- Efficient purification of fragmented DNA as small as 50 bp
- No PCR inhibition regardless of the sample volume processed
More Information
| NucleoMag cfDNA |
|
| Technology | Magnetic bead technology |
| Format | Highly reactive superparamagnetic beads |
| Processing | Manual or automated |
| Starting material | 1–10 ml human EDTA/Cell-Free DNA BCT plasma |
| Fragment size | 50 bp |
| Typical yield | Depending on sample source, storage, and quality |
| A260/280 | 1.6–1.9 |
| Elution volume | 50–200 µl |
| Preparation time | 55 min/24 preps (2 ml; excl. lysis)* |
| Binding capacity | 0.3 µg/µl beads |
*Established on a KingFisher Flex.
Applications
- Manual or automated isolation of circulating cell-free DNA from human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes
- Typical downstream applications: NGS, qPCR, ddPCR
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Cell-free DNA-seq library prep in under 2 hours
ThruPLEX Plasma-Seq Kit
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- Three-step, single-tube protocol can be completed in less than 2 hours
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Takara Bio USA, Inc.
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