Silica-membrane-based viral RNA/DNA and bacterial DNA isolation from veterinary samples—NucleoSpin VET
NucleoSpin VET enables efficient purification of viral RNA and DNA, as well as bacterial DNA, from a broad range of veterinary sample types. This single-spin, “one-for-all” solution has been developed for nucleic acid extraction for various sample matrices including serum, plasma, animal whole blood, tissue, feces, swabs, and other cell-free biological samples. All samples can be purified following the same standard protocol after sample lysis. The sample lysis pretreatment depends on the starting material and the target viral or bacterial sequence.
NucleoSpin VET enables efficient purification of viral RNA and DNA, as well as bacterial DNA, from a broad range of veterinary sample types. This single-spin, “one-for-all” solution has been developed for nucleic acid extraction for various sample matrices including serum, plasma, animal whole blood, tissue, feces, swabs, and other cell-free biological samples. All samples can be purified following the same standard protocol after sample lysis. The sample lysis pretreatment depends on the starting material and the target viral or bacterial sequence.
For rapid, routine testing of veterinary samples, the kit includes a FastTrack protocol for easy-to-lyse, low-complexity matrices (plasma, swabs). The FastTrack protocol is focused on speed and ease of use combined with maximum sensitivity.
Overview
- Employs one standard protocol for all specimen matrices after sample pretreatment
- Removes inhibitors and contaminants, even when working with difficult sample matrices (e.g. feces or fatty raw milk)
- Yields eluates that can be directly used for all common downstream analysis methods, including RT-qPCR and NGS
- Includes FastTrack protocol for rapid, routine veterinary testing
More Information
| Technology | Silica membrane technology |
| Format | Single-spin format |
| Sample amount | 200 μl serum 200 μl cell-free biological fluids, e.g. milk 5–10 mg tissue 100 μl animal blood 100 mg feces/stool 1 dry or wet swab |
| Fragment size | Approx. 100 bp–50 kb |
| Elution column volume | 40–100 μl |
| Processing time | 20–40 min |
Applications
- Extraction of viral RNA and DNA and bacterial DNA from veterinary samples
- Veterinary testing applications
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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