Purification of circulating DNA from plasma—NucleoSpin cfDNA XS
Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.
Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.
NucleoSpin cfDNA XS is designed to isolate fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit overcomes the limitations of conventional kits, namely large membrane diameters associated with large default elution volumes, and a resulting DNA concentration that is too low for direct use as a PCR template. NucleoSpin cfDNA XS allows elution in just 5–20 µl, resulting in highly concentrated DNA.
Overview
- High recovery of fragments >50 bp
- Elution in only 5 µl
- Concentrated DNA even from dilute samples
- DNA is ready to use for real-time PCR
More Information
|
Technology |
Silica membrane |
| Format | XS spin columns |
| Starting material | 240 µl plasma or serum |
| Fragment size | >50 bp |
| Typical yield | 0.1–100 ng per ml plasma, depending on sample |
| Typical concentration | 1.2–12,000 pg/µl |
| Elution volume | 5–20 µl |
| Preparation time |
High-sensitivity procedure: 22–27 min/6 preps |
Applications
- Purify circulating DNA from plasma or serum
- Analyze fetal DNA from maternal plasma
- Detect tumor markers in plasma
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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