NucleoMag NGS Clean-up and Size Select

Magnetic-bead based DNA cleanup and size selection for NGS library preparation

Cleanup of enzymatic reactions and size selection of DNA fragments are common steps in the vast majority of NGS library preparation workflows. NucleoMag NGS Clean-up and Size Select employs scalable magnetic bead technology to enable efficient cleanup and tunable size selection of NGS libraries for an array of sequencing applications. The flexible protocol allows for single- or double-sided size selection and enrichment of fragments of a given size via adjustment of bead-to-sample volume ratios.

NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications.

NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers (e.g., AMPure XP), such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

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Cat. #	Product	Size	Price
744970.5	NucleoMag® NGS Clean-up and Size Select	5 mL	$95.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of enzymatic reactions and tunable size selection of DNA fragments generated in NGS library preparation workflows. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150–800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-µl aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/µl) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-µg input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Sequencing was performed on a HiSeq 2500 using the TruSeq Rapid SBS Kit. Panel A. Genome sequence coverage distributions for NGS libraries prepared using Illumina (red), AMPure XP (blue), or NucleoMag (green) size-selection beads. Normalized read depths for mapped bases are binned along the X-axis. Frequencies of reference bases covered at the corresponding read depths are indicated on the Y-axis. Overlap of the coverage distributions indicates comparable performance of the respective size-selection beads. Panel B. Comparison of genome sequence coverage as a function of GC content for NGS libraries prepared using NucleoMag (MN) and AMPure XP size-selection beads. Ratios of mapped-base read depths for the respective NGS libraries were analyzed as a function of GC content using 0.5-kb windows. Ratios close to 1 indicate comparable representation of mapped reads in the datasets for the respective NGS libraries. Back 744970.5: NucleoMag NGS Clean-up and Size Select
744970.50	NucleoMag® NGS Clean-up and Size Select	50 mL	$645.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of enzymatic reactions and tunable size selection of DNA fragments generated in NGS library preparation workflows. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150–800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-µl aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/µl) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-µg input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Sequencing was performed on a HiSeq 2500 using the TruSeq Rapid SBS Kit. Panel A. Genome sequence coverage distributions for NGS libraries prepared using Illumina (red), AMPure XP (blue), or NucleoMag (green) size-selection beads. Normalized read depths for mapped bases are binned along the X-axis. Frequencies of reference bases covered at the corresponding read depths are indicated on the Y-axis. Overlap of the coverage distributions indicates comparable performance of the respective size-selection beads. Panel B. Comparison of genome sequence coverage as a function of GC content for NGS libraries prepared using NucleoMag (MN) and AMPure XP size-selection beads. Ratios of mapped-base read depths for the respective NGS libraries were analyzed as a function of GC content using 0.5-kb windows. Ratios close to 1 indicate comparable representation of mapped reads in the datasets for the respective NGS libraries. Back 744970.50: NucleoMag NGS Clean-up and Size Select
744970.500	NucleoMag® NGS Clean-up and Size Select	500 mL	$4495.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of enzymatic reactions and tunable size selection of DNA fragments generated in NGS library preparation workflows. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150–800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-µl aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/µl) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-µg input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Sequencing was performed on a HiSeq 2500 using the TruSeq Rapid SBS Kit. Panel A. Genome sequence coverage distributions for NGS libraries prepared using Illumina (red), AMPure XP (blue), or NucleoMag (green) size-selection beads. Normalized read depths for mapped bases are binned along the X-axis. Frequencies of reference bases covered at the corresponding read depths are indicated on the Y-axis. Overlap of the coverage distributions indicates comparable performance of the respective size-selection beads. Panel B. Comparison of genome sequence coverage as a function of GC content for NGS libraries prepared using NucleoMag (MN) and AMPure XP size-selection beads. Ratios of mapped-base read depths for the respective NGS libraries were analyzed as a function of GC content using 0.5-kb windows. Ratios close to 1 indicate comparable representation of mapped reads in the datasets for the respective NGS libraries. Back 744970.500: NucleoMag NGS Clean-up and Size Select

Overview

Processing of inputs as low as 17.5 pg and input volumes ranging from 50–150 µl
Tunable selection of DNA fragments ranging in size from 150–800 bp
Scalable, automation-friendly magnetic bead technology
Comparable performance and dilution ratios to beads from other providers (e.g., AMPure XP)

More Information

Technology	Magnetic bead technology
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Input material	Reaction mixtures from common NGS library preparation kits
Amount of input material	17.5 pg–5 µg
Input volume	50–150 µl
Typical recovery	80%
Elution volume	10–100 µl

Applications

Cleanup and size selection of DNA fragments during NGS library construction
Next-generation sequencing

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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NucleoMag NGS Clean-up and Size Select

Notice to purchaser

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.5: NucleoMag NGS Clean-up and Size Select

Notice to purchaser

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.50: NucleoMag NGS Clean-up and Size Select

Notice to purchaser

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.500: NucleoMag NGS Clean-up and Size Select

Overview

More Information

Applications

Additional product information