NucleoMag NGS Clean-up and Size Select
Cleanup of enzymatic reactions and size selection of DNA fragments are common steps in the vast majority of NGS library preparation workflows. NucleoMag NGS Clean-up and Size Select employs scalable magnetic bead technology to enable efficient cleanup and tunable size selection of NGS libraries for an array of sequencing applications. The flexible protocol allows for single- or double-sided size selection and enrichment of fragments of a given size via adjustment of bead-to-sample volume ratios.
Cleanup of enzymatic reactions and size selection of DNA fragments are common steps in the vast majority of NGS library preparation workflows. NucleoMag NGS Clean-up and Size Select employs scalable magnetic bead technology to enable efficient cleanup and tunable size selection of NGS libraries for an array of sequencing applications. The flexible protocol allows for single- or double-sided size selection and enrichment of fragments of a given size via adjustment of bead-to-sample volume ratios.
NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications.
NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers (e.g., AMPure XP), such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.
Overview
- Processing of inputs as low as 17.5 pg and input volumes ranging from 50–150 µl
- Tunable selection of DNA fragments ranging in size from 150–800 bp
- Scalable, automation-friendly magnetic bead technology
- Comparable performance and dilution ratios to beads from other providers (e.g., AMPure XP)
More Information
Technology | Magnetic bead technology |
Format | Highly reactive superparamagnetic beads |
Processing | Manual or automated |
Input material | Reaction mixtures from common NGS library preparation kits |
Amount of input material | 17.5 pg–5 µg |
Input volume | 50–150 µl |
Typical recovery | 80% |
Elution volume | 10–100 µl |
Applications
- Cleanup and size selection of DNA fragments during NGS library construction
- Next-generation sequencing
Studies performed using NucleoMag NGS Clean-up and Size Select
Tian, L. et al. Benchmarking single cell RNA-sequencing analysis pipelines using mixture control experiments. Nat Methods. 16, 479-487 (2019).
McKenzie, MD. et al. Interconversion between Tumorigenic and Differentiated States in Acute Myeloid Leukemia. Cell Stem Cell. 25, 258-272 (2019).
Kubik, S. et al. Opposing chromatin remodelers control transcription initiation frequency and start site selection. Nat Struct Mol Biol. 26, 744-754 (2019).
Alcala, N. et al. Integrative and comparative genomic analyses identify clinically relevant pulmonary carcinoid groups and unveil the supra-carcinoids. Nat Commun. 10, 3407 (2019).
Keller, A. et al. Wild bees and their nests host Paenibacillus bacteria with functional potential of avail. Microbiome. 6, 229 (2018).
Derks, JL. et al. Molecular Subtypes of Pulmonary Large-cell Neuroendocrine Carcinoma Predict Chemotherapy Treatment Outcome. Clin Cancer Res. 24, 33-42 (2018).
Challal, D. et al. General Regulatory Factors Control the Fidelity of Transcription by Restricting Non-coding and Ectopic Initiation. Mol Cell. 72, 955-969 (2018).
Chauvin, C. et al. NKG2D controls natural reactivity of Vγ9Vδ2 T lymphocytes against mesenchymal glioblastoma cells. Clin Cancer Res. 25, 7218-28 (2019).
Villalobos, A. et al. Systematic Affiliation and Genome Analysis of Subtercola vilae DB165T with Particular Emphasis on Cold Adaptation of an Isolate from a High-Altitude Cold Volcano Lake. Microorganisms. 7, 107 (2019).
Dagher, D. et al. Plant Identity Shaped Rhizospheric Microbial Communities More Strongly Than Bacterial Bioaugmentation in Petroleum Hydrocarbon-Polluted Sediments. Front Microbiol. 10, 2144 (2019).
Simon, B. et al. Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016-2017 Season. Viruses. 11, 108 (2019).
Wemheuer, F. et al. Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean. Archaea. 2019, 3717239 (2019).
Prazeres, M. Bleaching-Associated Changes in the Microbiome of Large Benthic Foraminifera of the Great Barrier Reef, Australia. Front Microbiol. 9, 2404 (2018).
Pohlner, M. et al. The Biogeographical Distribution of Benthic Roseobacter Group Members along a Pacific Transect Is Structured by Nutrient Availability within the Sediments and Primary Production in Different Oceanic Provinces. Front Microbiol. 8, 2550 (2017).
Horn, H. et al. Draft genome of the Arabidopsis thaliana phyllosphere bacterium, Williamsia sp. ARP1. Stand Genomic Sci. 11, 8 (2016).
Massilani, D. et al. Past climate changes, population dynamics and the origin of Bison in Europe. BMC Biol. 14, 93 (2016).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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