NucleoMag DNA Microbiome
The NucleoMag DNA Microbiome kit enables high-throughput DNA isolation for the study of microbial communities found in samples such as stool, soil, and biofilms. The kit employs a proprietary inhibitor removal technology to avoid challenges associated with PCR amplification of extracted DNA in downstream applications, and allows for the incorporation of a sample homogenization step to ensure sufficient DNA yields from hard-to-lyse samples.
The NucleoMag DNA Microbiome kit enables high-throughput DNA isolation for the study of microbial communities found in samples such as stool, soil, and biofilms. The kit employs a proprietary inhibitor removal technology to avoid challenges associated with PCR amplification of extracted DNA in downstream applications, and allows for the incorporation of a sample homogenization step to ensure sufficient DNA yields from hard-to-lyse samples.
The magnetic bead-based format of the kit enables easy automation of the sample processing workflow, and the protocol can be scaled up or down according to the demands of the application. Automated processing of 96 samples on the KingFisher Flex can be completed in ~30 min (excluding sample lysis). Automation scripts are available for common platforms and can be requested from our technical support team.
Overview
- Efficient processing of stool, soil, and biofilm samples—obtain higher yields than with competitor kits
- Proprietary PCR inhibitor removal technology—avoid additional processing steps and troubleshooting of downstream assays
- Accompanying homogenization solutions available—overcome challenges associated with hard-to-lyse samples through the incorporation of MN bead tubes or MN 96 bead plates
- Automation-friendly format—save time on high-throughput applications
More Information
| Technology | Magnetic-bead technology |
| Processing | Manual or automated |
| Sample material | Stool, soil, biofilms (e.g., swabs) |
| Sample amount | 50–200 mg |
| Elution volume | 50–100 µl |
| Preparation time | 30 min on KingFisher Flex (excluding sample lysis) |
Applications
- Isolation of DNA from stool, soil, and biofilm samples
- Typical downstream applications: PCR, qPCR, NGS
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Product demonstration
Learn how NucleoMag DNA Microbiome can be used to obtain pure, high-quality DNA by processing multiple soil samples manually in combination with the NucleoMag SEP Mini magnetic stand.
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