Rapid PCR cleanup: NucleoFast 96-well plates

96-well PCR clean-up kits and plates using ultrafiltration for fast and reliable purification of nucleic acids

The NucleoFast 96 PCR Clean-Up Kit and NucleoFast 96 PCR Plates use ultrafiltration for high recovery of nucleic acids in less than 20 minutes. The nucleic acids from PCR are collected on the filter membrane while the contaminants are filtered directly to the waste. The NucleoFast protocol is faster because it eliminates the intermediate wash steps required with silica-based purification systems. The 96 PCR Clean-Up Kit includes buffers and an elution plate. The sturdy, one-piece plate is easily adapted to automated processing. The purified PCR products are suitable for most common downstream applications, including capillary sequencing, microarray spotting, restriction analysis, labeling, or cloning.

Cat. #	Product	Size
743500.4	NucleoFast® 96 PCR Clean-Up Kit	4 x 96 Preps	USD $575.00
NucleoFast 96 PCR Clean-up Kit (4 x 96) 4 x 96 preps for the purification of PCR products. Includes 4 NucleoFast 96 PCR Plates, Elution Plates U-bottom, Self-adhering Foils, buffers Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoFast purification NucleoFast purification. The reaction mixture is loaded directly to the well. The DNA binds, while the contaminants (dNTPs, primers, salts, and other additives) pass through the filter membrane. No washing step required. The purified DNA is then recovered by resuspending with buffer or water. Back High recovery of purified DNA High recovery of purified DNA. The NucleoFast ultrafiltration membrane has a high rate of recovery of even small PCR products. The NucleoFast filter's most notable feature is a high recovery rate—40–95% for PCR products of 150-10,000 bp. Recovery rate depends on the PCR product length. Back 743500.4: NucleoFast 96 PCR Clean-Up Kit
743100.50	NucleoFast® 96 PCR Plates	50 Plates	USD $1745.00
NucleoFast 96 PCR Plates (50) 96-well plates for the purification of PCR products pack of 50 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoFast purification NucleoFast purification. The reaction mixture is loaded directly to the well. The DNA binds, while the contaminants (dNTPs, primers, salts, and other additives) pass through the filter membrane. No washing step required. The purified DNA is then recovered by resuspending with buffer or water. Back High recovery of purified DNA High recovery of purified DNA. The NucleoFast ultrafiltration membrane has a high rate of recovery of even small PCR products. The NucleoFast filter's most notable feature is a high recovery rate—40–95% for PCR products of 150-10,000 bp. Recovery rate depends on the PCR product length. Back 743100.50: NucleoFast 96 PCR Plates
743100.10	NucleoFast® 96 PCR Plates	10 Plates	USD $499.00
NucleoFast 96 PCR Plates (10) 96-well plates for the purification of PCR products pack of 10 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoFast purification NucleoFast purification. The reaction mixture is loaded directly to the well. The DNA binds, while the contaminants (dNTPs, primers, salts, and other additives) pass through the filter membrane. No washing step required. The purified DNA is then recovered by resuspending with buffer or water. Back High recovery of purified DNA High recovery of purified DNA. The NucleoFast ultrafiltration membrane has a high rate of recovery of even small PCR products. The NucleoFast filter's most notable feature is a high recovery rate—40–95% for PCR products of 150-10,000 bp. Recovery rate depends on the PCR product length. Back 743100.10: NucleoFast 96 PCR Plates
743100.01	NucleoFast® 96 PCR Plate	1 Plate	USD $59.00
A single NucleoFast 96 PCR Plate (1 x 96) for the purification of PCR products. Does not include buffers or other accessories. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back High recovery of purified DNA High recovery of purified DNA. The NucleoFast ultrafiltration membrane has a high rate of recovery of even small PCR products. The NucleoFast filter's most notable feature is a high recovery rate—40–95% for PCR products of 150-10,000 bp. Recovery rate depends on the PCR product length. Back 743100.01: NucleoFast 96 PCR Plate

Overview

Rapid, cost-efficient, 96-well ultrafiltration kit for PCR cleanup—NucleoFast 96 PCR

More Information

	NucleoFast 96 PCR
Technology	Ultrafiltration technology
Format	96-well plates
Processing	Manual or automated
Starting material	20–300 µl
Fragment size	>150 bp
Typical recovery	40–95%
A_260/280	1.70–1.80
Recovery volume	25–100 µl
Preparation time	20 min/plate (for 25-µl PCR reactions)

Applications

Ready-to-use DNA for sequencing and microarray spotting
Manual or automated use

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Rapid PCR cleanup: NucleoFast 96-well plates

Notice to purchaser

NucleoFast purification

High recovery of purified DNA

743500.4: NucleoFast 96 PCR Clean-Up Kit

Notice to purchaser

NucleoFast purification

High recovery of purified DNA

743100.50: NucleoFast 96 PCR Plates

Notice to purchaser

NucleoFast purification

High recovery of purified DNA

743100.10: NucleoFast 96 PCR Plates

Notice to purchaser

High recovery of purified DNA

743100.01: NucleoFast 96 PCR Plate

Overview

More Information

Applications

Additional product information