Rapid PCR cleanup: NucleoFast 96-well plates
The NucleoFast 96 PCR Clean-Up Kit and NucleoFast 96 PCR Plates use ultrafiltration for high recovery of nucleic acids in less than 20 minutes. The nucleic acids from PCR are collected on the filter membrane while the contaminants are filtered directly to the waste. The NucleoFast protocol is faster because it eliminates the intermediate wash steps required with silica-based purification systems. The 96 PCR Clean-Up Kit includes buffers and an elution plate. The sturdy, one-piece plate is easily adapted to automated processing. The purified PCR products are suitable for most common downstream applications, including capillary sequencing, microarray spotting, restriction analysis, labeling, or cloning.
Overview
Rapid, cost-efficient, 96-well ultrafiltration kit for PCR cleanup—NucleoFast 96 PCR
More Information
| NucleoFast 96 PCR | |
| Technology | Ultrafiltration technology |
| Format |
96-well plates |
| Processing | Manual or automated |
| Starting material | 20–300 µl |
| Fragment size | >150 bp |
| Typical recovery | 40–95% |
| A260/280 | 1.70–1.80 |
| Recovery volume | 25–100 µl |
| Preparation time | 20 min/plate (for 25-µl PCR reactions) |
Applications
- Ready-to-use DNA for sequencing and microarray spotting
- Manual or automated use
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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