Magnetic bead-based isolation of viral RNA/DNA and bacterial DNA from veterinary samples—NucleoMag VET
NucleoMag VET enables manual or automated purification of viral RNA and DNA and bacterial DNA using proven NucleoMag magnetic bead technology. The kit is designed for common veterinary sample material, such as animal whole blood, serum/plasma, feces, ear notches, tissue, or swabs. After a sample-specific pre-lysis procedure, microbial nucleic acids are purified from 200 µl of liquid or homogenized sample. The entire NucleoMag VET protocol can be automated on common liquid handling platforms.
NucleoMag VET enables manual or automated purification of viral RNA and DNA and bacterial DNA using proven NucleoMag magnetic bead technology. The kit is designed for common veterinary sample material, such as animal whole blood, serum/plasma, feces, ear notches, tissue, or swabs. After a sample-specific pre-lysis procedure, microbial nucleic acids are purified from 200 µl of liquid or homogenized sample. The entire NucleoMag VET protocol can be automated on common liquid handling platforms.
The NucleoMag principle is similar to silica membrane technology: nucleic acids are bound to magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA/DNA are eluted from the beads using elution buffer. NucleoMag technology can be used either with static-pin separators (e.g., NucleoMag SEP) or with magnetic separators integrated into robotic workstations.
Figure 1. Sensitive detection of viral DNA from animal tissue samples. Viral DNA was purified from PCV2-positive animal tissue samples (n = 22, 20 mg each) using NucleoMag VET and a kit from Competitor Q, respectively, and analyzed by qPCR. Of the 22 samples tested, 20 were found to be positive for PCV2 using NucleoMag VET, whereas only 14 were found to be positive using the kit from Competitor Q. The graph depicts mean CT values for amplification of the PCV2 sequence from viral DNA purified with either kit.
Figure 2. Sensitive detection of viral RNA and DNA from animal blood samples. Purified Viral RNA and DNA obtained from 200 µl blood serum samples of PRRSV- and PCV2-positive animals using NucleoMag VET was diluted 10-fold and analyzed by qPCR. For both RNA and DNA (blue and gray bars, respectively), CT values correlate with dilution factor. Sequences corresponding to PRRSV and PCV2 were detectable across the range of dilutions tested.
Overview
- One kit for common veterinary samples
- High sensitivity, even with low viral titers
- Robust, one-tube procedure minimizes risk of cross-contamination
- Reliable RNA/DNA isolation from different viral titers
More Information
| Technology | Magnetic bead technology |
| Format | Highly reactive superparamagnetic beads |
| Processing | Manual or automated |
| Starting material | <200 µl whole blood, serum, plasma <30 mg tissue (e.g., ear notches) <200 µl feces, <200 µl swab wash solution |
| Maximum amount of starting material in purification procedure |
200 µl liquid/homogenized sample |
| Fragment size | 300 bp–approximately 50 kb |
| Elution volume | 50–100 µl |
| Preparation time | 45–120 min/96 preps (45 min for KingFisher) |
| Binding capacity | 0.4 μg/μl beads |
Applications
- Extraction of viral RNA and DNA and bacterial DNA from veterinary samples
- Veterinary testing applications
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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