PCR cleanup: NucleoSpin 96-well plates
The NucleoSpin 96 PCR Clean-Up kits combine an improved buffer chemistry with the design of 96-well plates and allow the direct purification of PCR products using vacuum manifolds or benchtop centrifuges. The kits allow the rapid cleanup of up to 15 µg of DNA (65 bp–10 kb) per preparation with high DNA recovery and reproducible yields. After purification, the highly pure PCR products are ready for immediate use in many applications.
The NucleoSpin 96 PCR Clean-Up kits combine an improved buffer chemistry with the design of 96-well plates and allow the direct purification of PCR products using vacuum manifolds or benchtop centrifuges. The kits allow the rapid cleanup of up to 15 µg of DNA (65 bp–10 kb) per preparation with high DNA recovery and reproducible yields. After purification, the highly pure PCR products are ready for immediate use in many applications.
NucleoSpin PCR Clean-Up Binding Plates can be used under vacuum or by centrifugation. The NucleoSpin 96 PCR Clean-Up kit uses advanced silica-membrane technology. The addition of a binding buffer containing chaotropic ions leads to a reversible adsorption of DNA to the silica membrane. PCR products bind to the silica membrane under optimized conditions, while contaminants like primers, salts, nucleotides, and proteins are completely removed by effective washing. Highly pure PCR products are eluted with low-salt elution buffer (5 mM Tris/HCl pH 8.5) or water (pH 8.0–8.5) and are ready to use directly in subsequent applications.
Overview
- Time-saving parallel cleanup of PCR products
- Complete removal of primers and primer-dimers
- Optimal recovery
- Suitable for processing under vacuum and centrifugation
- Innovative MN Wash Plate minimizes risk of cross-contamination
- Suitable for manual or automated use
More Information
| Technology | Silica membrane |
| Format | 96-well plates |
| Processing | Manual or automated, vacuum or centrifugation |
| Starting material | <100 µl PCR reaction mixture |
| Fragment size | 65 bp–10 kb |
| Typical recovery | 75–95% |
| A260/280 | 1.70–1.80 |
| Elution volume | 75–150 µl |
| Preparation time | 45 min/plate |
| Binding capacity | 15 µg |
Applications
- Radioactive and fluorescent dye sequencing, including capillary sequencing
- Restriction analysis
- Ligation
- Microarray analysis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Detection of influenza A virus subtype H1N1
In this study, we describe the automated use of the NucleoMag Virus kit on the KingFisher Flex magnetic separator for the isolation of viral nucleic acids and subsequent detection by PCR. For kit evaluation, A/H1N1 samples from an interlaboratory test, as well as clinical samples, were used for kit evaluation. The data demonstrates the excellent quality of the eluted viral RNA which is suitable for downstream RT-PCR.
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