Endotoxin-free plasmid DNA purification—NucleoBond EF and RS buffers
Buffers needed for endotoxin-free plasmid isolation using NucleoBond Xtra Midi/Maxi*, NucleoBond AX†, or NucleoBond RS columns. Please click "More" for listings of the required and recommended buffers for each column type.
*These columns are used in the NucleoBond Xtra EF kits.
†These columns are used in the NucleoBond PC kits.
Buffers needed for endotoxin-free plasmid isolation or post-IVT RNA cleanup using NucleoBond Xtra Midi/Maxi*, NucleoBond AX†, or NucleoBond RS columns. Please click "More" for listings of the required and recommended buffers for each column type.
*These columns are used in the NucleoBond Xtra EF kits.
†These columns are used in the NucleoBond PC kits.
NucleoBond Xtra Midi/Maxi column buffers
| Product name | Size | Specification | Cat. # |
| Buffer H2O-EF | 1,000 mL | Water for reconstituting DNA | 740798.1 |
| Buffer TE-EF | 1,000 mL | Slightly alkaline, low-salt buffer for reconstituting DNA | 740797.1 |
| Buffer ELU-EF | 900 mL | Elution buffer | 740393.900 |
| Buffer WASH-EF | 1,000 mL | Wash buffer | 740392.1000 |
| Buffer ENDO-EF | 1,000 mL | Wash buffer ENDO | 740391.1000 |
| Buffer FIL-EF | 600 mL | Filter wash buffer | 740390.600 |
| Buffer EQU-EF | 1,000 mL | Equilibration buffer | 740389.1000 |
| Buffer NEU-EF | 1,000 mL | Neutralization buffer | 740388.1000 |
| Buffer LYS-EF | 1,000 mL | Lysis buffer | 740387.1000 |
| Buffer RES-EF | 1,000 mL | Resuspension buffer, without RNase A | 740386.1000 |
NucleoBond AX column buffers
| Product name | Size | Specification | Cat. # |
| Buffer S1-EF | 1,000 mL | Resuspension buffer, without RNase A | 740790.1 |
| Buffer S2-EF | 1,000 mL | Lysis buffer | 740791.1 |
| Buffer S3-EF | 1,000 mL | Neutralization buffer | 740792.1 |
| Buffer N2-EF | 1,000 mL | Equilibration buffer | 740793.1 |
| Buffer N3-EF | 1,000 mL | Wash buffer | 740794.1 |
| Buffer N4-EF | 1,000 mL | Wash buffer | 740795.1 |
| Buffer N5-EF | 600 mL | Elution buffer | 740796.1 |
NucleoBond RS column buffers
| Product name | Size | Specification | Cat. # |
| Buffer LYS-EF | 1,000 mL | Lysis buffer | 740387.1000 |
| Buffer NEU-EF | 1,000 mL | Neutralization buffer | 740388.1000 |
| Buffer EQ-EF | 1,000 mL 3,000 mL |
Equilibration buffer | 740380.1000 740380.3000 |
| Buffer ENTO-EF | 1,000 mL 3,000 mL |
Endotoxin removal buffer | 740381.1000 740381.3000 |
| Buffer WASH-EF | 1,000 mL | Wash buffer | 740392.1000 |
| Buffer ELU-EF | 900 mL | Elution buffer | 740393.900 |
| Buffer BMC-EF | 1,000 mL 3,000 mL |
Binding mediating reconstitution buffer for RNAse-free plasmid purification | 740382.1000 740382.3000 |
| Buffer NRB | 1,000 mL 3,000 mL |
Binding buffer for RNA cleanup | 740371.1000 740371.3000 |
| Buffer EQU* | 1,000 mL | Equilibration buffer for RNA cleanup | 740317.1000 |
| Buffer NRW | 1,000 mL 3,000 mL |
Wash buffer for RNA cleanup | 740372.1000 740372.3000 |
| Buffer NRE | 1,000 mL 3,000 mL |
Elution buffer for RNA cleanup | 740373.1000 740373.3000 |
| Buffer RES-EF | 1,000 mL | Resuspension buffer, without RNase A | 740386.1000 |
| Buffer TE-EF | 1,000 mL | Slightly alkaline, low-salt buffer for reconstituting DNA | 740797.1 |
| Buffer H2O-EF | 1,000 mL | Water for reconstituting DNA | 740798.1 |
*To purchase Buffer EQU, please refer to the NucleoBond buffers product page.
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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