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NucleoSpin consumables

Columns, collection tubes, and filters for use with NucleoSpin kits.

Cat. #	Product	Size	Price
740959	NucleoSpin® Funnel Column	30 Sets	USD $215.00
NucleoSpin Funnel Columns (30 sets) set of NucleoSpin Funnel Column, Collection Tube (50 mL), Collection Tube (0.5 mL) pack of 30 sets Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740959: NucleoSpin Funnel Column
740955.50S	NucleoSpin® RNA II Columns	50 Columns	USD $150.00
Columns for NucleoSpin RNA kits. NucleoSpin RNA is ideal for rapid purification of total RNA using a tabletop centrifuge. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin RNA II procedure NucleoSpin RNA II procedure. Back Residual Genomic DNA Comparison on an Agarose Gel Residual Genomic DNA Comparison on an Agarose Gel. 10 μl of each eluate was treated with RNase A and subsequently loaded onto a 1% TAE agarose gel to reveal any contaminating genomic DNA.The NucleoSpin RNA II kit contains DNase I for on-column digestion. For Competitor Q’s kit, an additional on-column DNA digestion set was purchased separately in order to perform the digest. Lane M: λ/HindIII DNA size marker. Note: Optimized DNase I treatment has eliminated genomic DNA in total RNA samples purified with the NucleoSpin RNA II kit. Even after DNA digestion, Competitor Q’s kit showed evidence of genomic DNA contamination. Back 740955.50S: NucleoSpin RNA II Columns
740955.250S	NucleoSpin® RNA II Columns	250 Columns	USD $705.00
Columns for NucleoSpin RNA kits. NucleoSpin RNA is ideal for rapid purification of total RNA using a tabletop centrifuge. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin RNA II procedure NucleoSpin RNA II procedure. Back Residual Genomic DNA Comparison on an Agarose Gel Residual Genomic DNA Comparison on an Agarose Gel. 10 μl of each eluate was treated with RNase A and subsequently loaded onto a 1% TAE agarose gel to reveal any contaminating genomic DNA.The NucleoSpin RNA II kit contains DNase I for on-column digestion. For Competitor Q’s kit, an additional on-column DNA digestion set was purchased separately in order to perform the digest. Lane M: λ/HindIII DNA size marker. Note: Optimized DNase I treatment has eliminated genomic DNA in total RNA samples purified with the NucleoSpin RNA II kit. Even after DNA digestion, Competitor Q’s kit showed evidence of genomic DNA contamination. Back 740955.250S: NucleoSpin RNA II Columns
740952.250S	NucleoSpin® Tissue Columns	250 Columns	USD $336.00
NucleoSpin Tissue Columns are mini spin columns intended for use with the NucleoSpin Tissue kits for the isolation of genomic DNA from a wide array of cells and tissues. Cat. # 740952.250S consists of a pack of 250 columns. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections. Back The NucleoSpin Tissue procedure The NucleoSpin Tissue procedure. Back 740952.250S: NucleoSpin Tissue Columns
740770.250S	NucleoSpin® Plant II Columns	250 Columns	USD $444.00
Columns for NucleoSpin Plant II kits. NucleoSpin Plant II is ideal for rapid purification of plant genomic DNA using a tabletop centrifuge. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue. The two different lysis buffers included in the kit allow you to purify a wide range of starting material. Back 740770.250S: NucleoSpin Plant II Columns
740705	bondEX™ Folded Filters XL	50 Filters	USD $51.00
bondEX Folded Filters XL (50) pack of 50 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740705: bondEX Folded Filters XL
740609.50S	NucleoSpin® Gel and PCR Clean-Up Columns	50 Columns	USD $52.00
NucleoSpin Gel and PCR Clean-up Columns (50) binding columns for gel extraction and PCR clean-up pack of 50 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5). Back 740609.50S: NucleoSpin Gel and PCR Clean-Up Columns
740609.250S	NucleoSpin® Gel and PCR Clean-Up Columns	250 Columns	USD $243.00
NucleoSpin Gel and PCR Clean-up Columns (250) binding columns for gel extraction and PCR clean-up pack of 250 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes. Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products. The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5). Back 740609.250S: NucleoSpin Gel and PCR Clean-Up Columns
740606	NucleoSpin® Filter Units	50 Each	USD $98.00
NucleoSpin Filters (50) columns for filtration of cell homogenates (50) and collection tubes (50) Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Image Data Back 740606: NucleoSpin Filter Units
740600	NucleoSpin® Collection Tubes	1,000 Tubes	USD $117.00
Collection Tubes (1000) Collection Tubes 2 mL, without lid pack of 1000 Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Image Data Back 740600: NucleoSpin Collection Tubes
740588.50S	NucleoSpin® Plasmid Binding Columns	50 Columns	USD $43.00
Columns for NucleoSpin Plasmid kits. NucleoSpin Plasmid is ideal for rapid purification of plasmid DNA using a tabletop centrifuge. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back The NucleoSpin Plasmid miniprep protocol The NucleoSpin Plasmid miniprep protocol. Back 740588.50S: NucleoSpin Plasmid Binding Columns
740588.250S	NucleoSpin® Plasmid Binding Columns	250 Columns	USD $172.00
Columns for NucleoSpin Plasmid kits. NucleoSpin Plasmid is ideal for rapid purification of plasmid DNA using a tabletop centrifuge. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back The NucleoSpin Plasmid miniprep protocol The NucleoSpin Plasmid miniprep protocol. Back 740588.250S: NucleoSpin Plasmid Binding Columns
740499.50S	NucleoSpin® Plasmid Binding Columns (No Lid)	50 Columns	USD $43.00
Columns for NucleoSpin Plasmid kits. NucleoSpin Plasmid is ideal for rapid purification of plasmid DNA using a tabletop centrifuge. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back The NucleoSpin Plasmid miniprep protocol The NucleoSpin Plasmid miniprep protocol. Back 740499.50S: NucleoSpin Plasmid Binding Columns (No Lid)
740499.250S	NucleoSpin® Plasmid Binding Columns (No Lid)	250 Columns	USD $172.00
Columns for NucleoSpin Plasmid kits. NucleoSpin Plasmid is ideal for rapid purification of plasmid DNA using a tabletop centrifuge. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back The NucleoSpin Plasmid miniprep protocol The NucleoSpin Plasmid miniprep protocol. Back 740499.250S: NucleoSpin Plasmid Binding Columns (No Lid)
740304	NucleoSpin miRNA Column/Buffer Set	50 Preps	USD $238.00
The NucleoSpin miRNA Column/Buffer Set consists of 50 NucleoSpin columns, 35 ml of Wash Buffer MW1, and 20 ml of Wash Buffer MW2. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740304: NucleoSpin miRNA Column/Buffer Set
740952.50S	NucleoSpin® Tissue Columns	50 Columns	USD $78.00
NucleoSpin Tissue Columns are mini spin columns intended for use with the NucleoSpin Tissue kits for the isolation of genomic DNA from a wide array of cells and tissues. Cat. # 740952.50S consists of a pack of 50 columns. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg. Up to 35 µg of pure genomic DNA can be prepared from two 0.5-cm mouse tail tip sections. Back The NucleoSpin Tissue procedure The NucleoSpin Tissue procedure. Back 740952.50S: NucleoSpin Tissue Columns
740956.250S	NucleoSpin® RNA Virus Columns	250 Columns	USD $487.00
Columns for isolation of viral RNA from serum, plasma, or any cell-free biological fluid. Also provided as a component of NucleoSpin RNA Virus kits. Cat. # 740956.250S consists of 250 silica membrane spin columns. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740956.250S: NucleoSpin RNA Virus Columns
740401.50	MN Sterilizer CA	50 Preps	USD $147.00
MN Sterilizer CA syringe filters enable sterile filtration and removal of particles from aqueous solutions. The cellulose acetate filter membrane has a diameter of 28 mm and a pore size of 0.2 μm. The syringe filters are sterilized with ethylene oxide and are stable at temperatures up to 50°C and pressures up to 4.5 bar. Cat. # 740401.50 includes 50 syringe filters. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740401.50: MN Sterilizer CA

Overview

In accordance with Macherey-Nagel´s Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.

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Product name	Quantity	Specification	Cat. #
NucleoSpin Gel and PCR Clean-Up Columns	50 250	Columns only (no buffers) for PCR purification or gel extraction applications	740609.50S 740609.250S
NucleoSpin Plasmid Binding Columns	50 250	Columns only (no buffers) for miniprep plasmid purification	740588.50S 740588.250S
NucleoSpin Tissue Columns	50 250	Columns only (no buffers) for gDNA purification from tissues	740952.50S 740952.250S
NucleoSpin Plant II Columns	250	Columns only (no buffers) for gDNA purification from plant tissue	740770.250S
NucleoSpin RNA II Columns	50 250	Columns only (no buffers) for total RNA isolation	740955.50S 740955.250S
NucleoSpin RNA Virus Columns	250	Columns only (no buffers) for viral RNA isolation	740956.250S
Collection Tubes (2 ml)	1,000		740600
NucleoSpin Filters (shredders)	50	For filtration of cell and tissue homogenates	740606
NucleoSpin Funnel Column	30 sets	Set of NucleoSpin Funnel Column, Collection Tube (50 ml), and Collection Tube (0.5 ml)	740959
BondEx Folded Filters XL	50		740705
MN Sterilizer CA	50	Syringe filters for sterile filtration and removal of particles from aqueous solutions; 28-mm membrane diameter, 0.2-µm pore size	740401.50
NucleoSpin Plasmid Binding Columns (No Lid)	50 250	Columns only (no buffers) for plasmid purification	740499.50S 740499.250S
NucleoSpin miRNA Column/Buffer Set	50	NucleoSpin columns, 35 ml of Wash Buffer MW1, and 20 ml of Wash Buffer MW2	740304

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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NucleoSpin consumables

Notice to purchaser

740959: NucleoSpin Funnel Column

Notice to purchaser

NucleoSpin RNA II procedure

Residual Genomic DNA Comparison on an Agarose Gel

740955.50S: NucleoSpin RNA II Columns

Notice to purchaser

NucleoSpin RNA II procedure

Residual Genomic DNA Comparison on an Agarose Gel

740955.250S: NucleoSpin RNA II Columns

Notice to purchaser

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

The NucleoSpin Tissue procedure

740952.250S: NucleoSpin Tissue Columns

Notice to purchaser

NucleoSpin Plant II is designed for the rapid isolation of genomic DNA from plant cells and tissue

740770.250S: NucleoSpin Plant II Columns

Notice to purchaser

740705: bondEX Folded Filters XL

Notice to purchaser

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

740609.50S: NucleoSpin Gel and PCR Clean-Up Columns

Notice to purchaser

Schematic of the NucleoSpin and NucleoTrap extraction protocols for isolation of DNA fragments from agarose gels or PCR mixes

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products

740609.250S: NucleoSpin Gel and PCR Clean-Up Columns

Notice to purchaser

740606: NucleoSpin Filter Units

Notice to purchaser

740600: NucleoSpin Collection Tubes

Notice to purchaser

The NucleoSpin Plasmid miniprep protocol

740588.50S: NucleoSpin Plasmid Binding Columns

Notice to purchaser

The NucleoSpin Plasmid miniprep protocol

740588.250S: NucleoSpin Plasmid Binding Columns

Notice to purchaser

The NucleoSpin Plasmid miniprep protocol

740499.50S: NucleoSpin Plasmid Binding Columns (No Lid)

Notice to purchaser

The NucleoSpin Plasmid miniprep protocol

740499.250S: NucleoSpin Plasmid Binding Columns (No Lid)

Notice to purchaser

740304: NucleoSpin miRNA Column/Buffer Set

Notice to purchaser

Typical yields of pure genomic DNA from NucleoSpin Tissue range from 15–25 µg

The NucleoSpin Tissue procedure

740952.50S: NucleoSpin Tissue Columns

Notice to purchaser

740956.250S: NucleoSpin RNA Virus Columns

Notice to purchaser

740401.50: MN Sterilizer CA

Overview

More Information

Additional product information