Plasmid DNA purification—NucleoBond buffers
Choose buffers for anion exchange columns for plasmid and BAC isolation using NucleoBond kits.
More Information
In accordance with Macherey-Nagel´s Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.
NucleoBond Xtra buffers (for Xtra Midi/Maxi preps)
| Product name | Size | Specification | Cat. # |
| NucleoBond Xtra Buffer Set I | 1 set | For isolation of low-copy plasmids, cosmids, BACs, PACs & P1 constructs. Only applicable to NucleoBond Xtra kits. Sufficient for 10 Xtra Maxi preps or 20 Xtra Midi preps when used in conjunction with the buffers included in the prep kit. Set includes buffers RES, LYS, NEU, and RNase A. |
740417 |
| Buffer RES | 1,000 ml | Resuspension buffer, without RNase A | 740363.1000 |
| Buffer LYS | 1,000 ml | Lysis buffer | 740329.1000 |
| Buffer NEU | 1,000 ml | Neutralization buffer | 740348.1000 |
| Buffer EQU | 1,000 ml | Equilibration buffer | 740317.1000 |
| Buffer WASH | 1,000 ml | Wash buffer | 740375.1000 |
| Buffer ELU | 600 ml | Elution buffer | 740316.600 |
NucleoBond buffers (for AX Columns and PC Midi/Maxi preps)
| Product name | Size | Specification | Cat. # |
| NucleoBond Buffer Set I |
1 set | For isolation of low-copy plasmids, cosmids, BACs, PACs & P1 constructs. Only applicable to NucleoBond PC kits. Sufficient for 10 AX 500 columns (Maxi preps) or 20 AX 100 columns (Midi preps) when used in conjunction with the buffers included with the columns. |
740601 |
| NucleoBond Buffer N2 |
500 ml | Equilibration buffer | 740527.1 |
| NucleoBond Buffer N3 |
1 L | Wash buffer | 740528.1 |
| NucleoBond Buffer N5 |
500 ml | Elution buffer | 740529.1 |
| NucleoBond Buffer S1 |
500 ml | Resuspension buffer, without RNase A | 740516.1 |
| NucleoBond Buffer S2 |
500 ml | Lysis buffer | 740517.1 |
| NucleoBond Buffer S3 |
500 ml | Neutralization buffer | 740518.1 |
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the product User Manual for protocols. Certificates of Analysis and User Manuals are located under the Documents tab.
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