One-step, universal lysis for direct PCR/qPCR—NucleoType DirectLyse Reagent
Streamline and simplify direct PCR/qPCR workflows with NucleoType DirectLyse Reagent, a universal lysis buffer for DNA extraction that eliminates the need for column-based isolation. NucleoType DirectLyse Reagent effectively releases DNA from a wide variety of samples while reducing PCR inhibitors in as little as 10–15 min. Extracted DNA can be used directly for PCR or qPCR analysis without the need for additional cleanup.
Overview
- Prepare samples for direct PCR/qPCR from a wide range of materials, including ear punches, tail clips, blood, hair, hair follicles, plant tissue, and swabs
- Simplify sample processing with a single-tube, column-free workflow
- Save time with an extremely fast (10–15 min) protocol
More Information
| Format | Column-free lysis reagent for manual use |
| Sample material | Animal tissue, blood, blood spots, blood storage cards, cells, ear punches, food, hair samples, plant tissue, seeds, swabs |
| Sample amount | Up to ~200 mg biological sample material |
| Elution/final volume | 20–500 µl |
| Preparation time | 10–15 min |
Applications
- Genotyping
- Knockout analysis
- Transgene detection
- GMO and pathogen detection
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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