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TCR and BCR profiling tips 4 factors to consider for TCR/BCR profiling
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TCR and BCR profiling tips 4 factors to consider for TCR/BCR profiling
NGS product line overview NGS portfolio overview

Monitoring the immune response to viral infection

Immune repertoire profiling

During viral pandemics, next-generation sequencing (NGS) can be a valuable tool to help researchers and clinicians understand immune response to infection and treatments. We offer a variety of RNA-sequencing kits for unmatched sensitivity and reproducibility for all of your demanding sequencing applications, including COVID-19 research.

To understand host response when a virus enters the body and later when a treatment is administered, it is important to understand how the transcriptional profiling of a host cell changes. For SARS-CoV-2, in particular, it will be important to see how the B cells and T cells are affected to understand what is going on with the immune system. Several questions remain to be answered, such as: are there changes in the clonotype numbers? What clonotypes are present? What effect does the infection have on the diversity of these clonotypes? When treatment is administered, how does the clonotype diversity change? Our human BCR and TCR kits enable you to answer these and other questions and help ensure we can respond properly now and are prepared for the future.

Cat. # Product Size Price License Quantity Details
634466 SMARTer® Human BCR IgG IgM H/K/L Profiling Kit 12 Rxns USD $1122.00

License Statement

ID Number  
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
455 This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit enables users to analyze B-cell receptor (BCR) repertoires from total RNA samples. This kit can generate libraries from 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) and 1 to 100 ng of total RNA from B cells. This kit may be used for other sample types, but the protocol may need to be modified. The kit can be used to generate data for both heavy and light chains (kappa and lambda) of human immunoglobulin IgG and IgM.

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of BCR transcripts. It also incorporates unique molecular identifiers (UMI) to facilitate PCR error correction and clonotype quantification during data analysis.

Included in the kit are primers that incorporate Illumina-specific adapter sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms. This kit supports up to 12 rxns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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634466: SMARTer Human BCR IgG IgM H/K/L Profiling Kit

634466: SMARTer Human BCR IgG IgM H/K/L Profiling Kit
634478 SMARTer® Human TCR a/b Profiling Kit v2 12 Rxns USD $1578.00

License Statement

ID Number  
455 This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

The SMARTer Human TCR a/b Profiling Kit v2 enables users to analyze T-cell receptor (TCR) repertoires from bulk RNA samples. This kit can generate libraries from total RNA (10 ng to 1 µg from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, or 1 ng to 100 ng T cells) or purified T cells (1,000 to 10,000 cells) and can be used to generate data for both TCR-alpha or TCR-beta subunits. Unique molecular identifiers (UMIs) are incorporated to facilitate PCR error correction and clonotype quantification during data analysis, and unique dual indexes (UDIs) allow users to multiplex more samples and to provide confidence when sequencing on patterned flow cells. At the end, generated indexed libraries are ready for sequencing on Illumina platforms.

This kit supports up to 12 rxns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Sensitive and reproducible clonotype detection from a broad range of sample types and RNA amounts.

Sensitive and reproducible clonotype detection from a broad range of sample types and RNA amounts.

Variable and highly complex samples are accommodated without the need for RNA purification or T-cell enrichment. TRA and TRB libraries were generated from 1, 10, and 100 ng of human CD3+ T‑cell total RNA (Panel A), 1,000 and 10,000 CD3+ T cells (Panel B), and 20 ng of whole-blood RNA extracted from three different samples (Panel C). The sequence reads were processed by Cogent NGS Immune Profiler.

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The Takara Bio TCR profiling kit identifies a wide range of clonotype counts.

The Takara Bio TCR profiling kit identifies a wide range of clonotype counts.

The number of clonotypes detected reveals biological variation. Duplicate libraries were generated from 10 ng RNA extracted from single-donor PBMC samples (P1-P6) and sequenced on an Illumina MiSeq system using 300-bp paired-end reads to obtain approximately 1.5 million reads per sample. Resulting sequencing reads were processed with Cogent NGS Immune Profiler. Clonotype numbers from different donors for TRA (blue) and TRB (orange) are shown. Error bars shown represent the standard error between the duplicates.

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The Takara Bio TCR profiling kit generates data with sensitivity superior to competitors

The Takara Bio TCR profiling kit generates data with sensitivity superior to competitors
A dramatically higher clonotype number was observed for TRB after downsampling. (TRA results were similar; data not shown.) 5M PBMC cells were split from two different healthy donors for RNA and gDNA extraction. 1.6 g of gDNA (15% of the total amount of extracted gDNA) was used for library preparation according to manufacturer's instructions. 100 ng of RNA (2% of the total amount of extracted RNA) was used for library preparation.

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634478: SMARTer Human TCR a/b Profiling Kit v2

634478: SMARTer Human TCR a/b Profiling Kit v2
634479 SMARTer® Human TCR a/b Profiling Kit v2 48 Rxns USD $4187.00

License Statement

ID Number  
455 This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

The SMARTer Human TCR a/b Profiling Kit v2 enables users to analyze T-cell receptor (TCR) repertoires from bulk RNA samples. This kit can generate libraries from total RNA (10 ng to 1 µg from peripheral blood leukocytes, 20 ng to 200 ng of total RNA obtained from whole blood, or 1 ng to 100 ng T cells) or purified T cells (1,000 to 10,000 cells) and can be used to generate data for both TCR-alpha or TCR-beta subunits. Unique molecular identifiers (UMIs) are incorporated to facilitate PCR error correction and clonotype quantification during data analysis, and unique dual indexes (UDIs) allow users to multiplex more samples and to provide confidence when sequencing on patterned flow cells. At the end, generated indexed libraries are ready for sequencing on Illumina platforms.

This kit supports up to 48 rxns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Sensitive and reproducible clonotype detection from a broad range of sample types and RNA amounts.

Sensitive and reproducible clonotype detection from a broad range of sample types and RNA amounts.

Variable and highly complex samples are accommodated without the need for RNA purification or T-cell enrichment. TRA and TRB libraries were generated from 1, 10, and 100 ng of human CD3+ T‑cell total RNA (Panel A), 1,000 and 10,000 CD3+ T cells (Panel B), and 20 ng of whole-blood RNA extracted from three different samples (Panel C). The sequence reads were processed by Cogent NGS Immune Profiler.

Back

The Takara Bio TCR profiling kit identifies a wide range of clonotype counts.

The Takara Bio TCR profiling kit identifies a wide range of clonotype counts.

The number of clonotypes detected reveals biological variation. Duplicate libraries were generated from 10 ng RNA extracted from single-donor PBMC samples (P1-P6) and sequenced on an Illumina MiSeq system using 300-bp paired-end reads to obtain approximately 1.5 million reads per sample. Resulting sequencing reads were processed with Cogent NGS Immune Profiler. Clonotype numbers from different donors for TRA (blue) and TRB (orange) are shown. Error bars shown represent the standard error between the duplicates.

Back

The Takara Bio TCR profiling kit generates data with sensitivity superior to competitors

The Takara Bio TCR profiling kit generates data with sensitivity superior to competitors
A dramatically higher clonotype number was observed for TRB after downsampling. (TRA results were similar; data not shown.) 5M PBMC cells were split from two different healthy donors for RNA and gDNA extraction. 1.6 g of gDNA (15% of the total amount of extracted gDNA) was used for library preparation according to manufacturer's instructions. 100 ng of RNA (2% of the total amount of extracted RNA) was used for library preparation.

Back

634479: SMARTer Human TCR a/b Profiling Kit v2

634479: SMARTer Human TCR a/b Profiling Kit v2

Overview

For additional product information and a full listing of available sizes, please visit:

  • SMARTer Human (bulk) BCR profiling product page
  • SMARTer human bulk TCRv2 and single-cell TCR profiling product pages
  • Cogent NGS Immune Profiler Software (bulk) and SMARTer Human scTCR Demultiplexer (single-cell) bioinformatics information pages

More Information

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Profiling human B-cell receptors with SMART technology

Analyze BCR repertoires from human PBMCs or purified B cells.

Human TCRv2 profiling kit for Illumina sequencing

SMARTer TCR profiling with optimized chemistry, UMIs, UDIs, and bioinformatics support for more accurate, reliable clonotype calling and quantification.


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
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  • cDNA synthesis kits
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  • Purified total RNA and mRNA
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  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
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