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  • hiPSC-based viral infection models
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ADME-Tox COVID-19 drug discovery: ADME-Tox studies
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hiPSC-based viral infection models

SARS-CoV-2 infection

SARS-CoV-2 primarily infects human airway epithelial cells, but there is increasing evidence that the virus can also infect other cell types that express the ACE2 receptor, such as cardiomyocytes, hepatocytes, and pancreatic endocrine cells (Yang et al. 2020). As you work tirelessly to understand COVID-19 pathogenesis, we can support your research with our hiPSC-derived cell models. These complete culture systems, including hiPSC-derived hepatocytes, beta cells, cardiomyocytes, and intestinal epithelial cells, aid in understanding viral infection and transmission mechanisms as well as identifying novel methods for blocking COVID-19 progression. Our hiPSC-derived cells display functional and mature characteristics of their primary-cell counterparts. They are ideal model systems for generating meaningful, reproducible results so you can quickly advance your COVID-19 research.

Hepatocytes | Beta cells | Cardiomyocytes | Intestinal epithelial cells | All products


Hepatocytes

Cellartis enhanced hiPS-HEP v2 cells provide an inexhaustible, consistent supply of functional and mature hepatocytes that can be used to study hepatocyte function over a 14-day assay window, making them ideal for metabolic disease modeling, drug discovery, and studies of drug metabolism and toxicity. They display important characteristics of mature hepatocytes, including expression of key hepatocyte markers (see figure below), secretion of albumin and urea, and functional regulation of glucose and lipids. If you want to study the effects of genetic background on infection and transmission, you can generate hepatocytes from your own hiPSC lines using our Cellartis iPS Cell to Hepatocyte Differentiation System.

Enhanced hiPS-HEP cells display markers of mature hepatocytes.

Enhanced hiPS-HEP cells display markers of mature hepatocytes. mRNA expression analysis of key markers of hepatocyte function, as quantified by RNA-seq. Panel A. ALB and α1AT levels were measured in enhanced hiPS-HEP cells derived from three different iPS cell lines (C12, C18, and C22) on Day 12 post-thawing (n = 2 batches per line) and compared to cryopreserved primary hepatocytes (hphep cells) on Day 1 post-thawing (n = 3 donors). Panel B. Urea cycle enzyme mRNA levels were measured from the same cell lines at the same timepoints and sample sizes. Abbreviations: ALB = albumin; α1AT = alpha-1-antitrypsin; CPS1 = carbamoyl-phosphate synthase; OTC = ornithine carbamoyltransferase; ASS1 = argininosuccinate synthase; ASL = argininosuccinate lyase; and ARG1 = arginase-1. Data are presented as mean values ± SEM.

View the full dataset »


Beta cells

Cellartis hiPS beta cells are generated using a standardized differentiation protocol that mimics embryonic development. These cells have been used for applications including compound screening for insulin secretion and regulation, GSIS analysis, and incretin response studies. We offer two complete kits, each containing cells that originate from healthy donors, including one with a diabetes-susceptible HLA type (HLA-A*02:01). Both kits offer a virtually unlimited source of cells that recapitulate key beta cell functions; they express beta cell-specific genes (see figure below), express insulin and C-peptide, and display an appropriate response to drugs that target the insulin pathway, allowing a more realistic study of beta-cell function in the face of a viral challenge.

hiPS cell-derived beta cells consistently express key beta cell mRNAs.

hiPS cell-derived beta cells consistently express key beta cell mRNAs. mRNA expression analysis, as quantified by RNA-seq, was performed on Cellartis beta cells derived from two different iPS cell lines (ChiPSC12 and ChiPSC22) on Day 14 post-thawing (n = 3 batches per cell line) and on primary islets (n = 5 donors). Abbreviations: PDX1 = pancreatic and duodenal homeobox 1; GCK = glucokinase; SLC2A1, SLC2A3 = Solute Carrier Family 2 Member 1 and 3; MAFB = MAF BZIP Transcription Factor B; NKX2.2 = NK2 homeobox 2; NKX6.1 = NK6 homeobox 1; and NEUROD1 = Neurogenic differentiation 1. Data are presented as the mean values ± SEM.

View the full dataset »


Cardiomyocytes

Cellartis cardiomyocytes are a highly homogeneous population of cardiomyocytes derived from human iPS cells that have been used for applications such as phenotypic screening, discovery of novel drug targets, safety pharmacology, and cardiotoxicity testing. The cells display an electrophysiological profile that highly resembles adult human primary cardiomyocytes; respond as expected to cardiac stimuli; and are derived with a robust, efficient cardiac differentiation protocol, without genetic engineering or selection. These cells have been used successfully in the generation of human-engineered heart tissue and for high-throughput electrophysiological analysis.

Cellartis cardiomyocytes can be used to generate engineered heart tissue (EHT).

Cellartis cardiomyocytes can be used to generate engineered heart tissue (EHT). Panel A. Live image of an EHT during contraction analysis. The EHT is spanning between two flexible silicone posts where optical figure recognition (blue crosses) is based and illuminated by an LED. Scale bar = 1 mm. Panel B. Exemplary contraction peaks of a spontaneously beating EHT showing contraction force over time.

View the full dataset »


Intestinal epithelial cells

Cellartis intestinal epithelial cells are small intestinal epithelial cells derived from human iPS cells that have been used for evaluation of drug absorption and metabolism in the small intestine, ADME profiling in drug discovery, and drug permeability assays. The cells form a functional, permeable barrier and express villin and CDX2. In addition, the cells have higher expression of the metabolic enzyme CYP3A4 and transporter PEPT1 compared to the human colon cancer Caco-2 cell line. This allows for better predictive results when evaluating metabolic absorption and drug interactions.

Cellartis intestinal epithelial cells recapitulate the microenvironment of the small intestine.

Cellartis intestinal epithelial cells recapitulate the microenvironment of the small intestine. Immunostaining for tight junction protein ZO-1 shows the formation of intercellular tight junctions (i.e., zonula occludens) by Day 5 post-thawing.

View the full dataset (Image Data tab in product table) »


Featured products

Product Cat. # View data
Cellartis Enhanced hiPS-HEP v2 Y10133
Y10134
Y10135
Technical note
Cellartis iPS Cell to Hepatocyte Differentiation System Y30055 Technical note
Webinar
Cellartis hiPS Beta Cells Y10100
Y10106
Technical note
Webinar
Cellartis Cardiomyocytes Y10075 Technical note
Brochure
Cellartis Intestinal Epithelial Cells Y50035 Image Data tab in product table

References

Yang, L. et al. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids. Cell Stem Cell 27, 125–136 (2020).

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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