We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Clontech Takara Cellartis

Close

  • ‹ Back to Hepatocytes
  • hiPS-HEP cells for disease modeling
  • hiPS-HEP cells for drug metabolism studies
  • Power medium for long-term human primary hepatocyte culture
  • iPS cell to hepatocyte differentiation system
Home › Learning centers › Stem cell research › Technical notes › Hepatocytes › iPS cell to hepatocyte differentiation system

Technical notes

  • Pluripotent stem cells
    • Using the DEF-CS system to culture human iPS cells
    • Comparison of the Cellartis DEF-CS system with other vendors' human iPS cell culture systems
    • Reprogramming PBMCs
    • Reprogramming fibroblasts
  • Gene editing in hiPS cells
    • Tagging an endogenous gene with AcGFP1 in hiPS cells
    • Tagging an endogenous gene with a myc tag in hiPS cells
    • Generating clonal hiPS cell lines deficient in CD81
    • Introducing a tyrosinemia-related SNP in hiPS cells
    • Inserting an expression cassette into the AAVS1 locus in hiPS cells
    • Editing hiPS cells using electroporation
    • Editing hiPS cells using gesicle technology
    • Single-cell cloning of hiPS cells
  • Organoids
    • Retinal organoid differentiation from iPSCs cultured in the Cellartis DEF-CS 500 Culture System
    • Liver organoid differentiation from iPSCs for prediction of drug-induced liver injury
    • Generation of embryonic organoids using NDiff 227 neural differentiation medium
  • Beta cells
    • Beta cells for disease modeling
  • Hepatocytes
    • hiPS-HEP cells for disease modeling
    • hiPS-HEP cells for drug metabolism studies
    • Power medium for long-term human primary hepatocyte culture
    • iPS cell to hepatocyte differentiation system
  • Cardiomyocytes
    • Making engineered heart tissue with cardiomyocytes
  • Neural stem cells
    • RHB-A neural stem cell medium
New products
Need help?
Contact Sales
Tech Note

A simple and complete system to generate mature hepatocytes from various iPS cell sources

  • Follow a standardized protocol with ready-to-use media and supplements to create panels of iPSC-derived hepatocytes
  • Generate >90% pure hepatocytes that express mature hepatic markers and show relevant CYP activities
  • Start with any patient-specific or disease-relevant iPS cell line
Introduction Results Conclusions Methods References

Introduction  

Human hepatocytes are highly attractive candidates for cell-based therapy for chronic liver disease and are essential to many studies in areas such as disease modeling, hepatitis research, and alcoholic fatty liver disease. However, the use of human hepatocytes is limited by their lack of availability and large inter-individual variation (Behbahan et al. 2011). These challenges also affect drug development, where there is a lack of robust and predictive cellular models during preclinical development and safety and toxicology evaluation. This, in turn, results in an enormous failure rate for newly developed drugs and substantial loss of both time and money for the pharmaceutical industry.

One way to circumvent the dearth of primary human hepatocytes is to generate them from induced pluripotent stem (iPS) cells. This method offers the unique possibility of an unlimited source of hepatocytes from various human donors. However, current hepatocyte differentiation protocols exhibit varying differentiation efficiencies across diverse iPS cell lines derived from multiple donor sources (Kajiwara et al. 2012). Moreover, in order to be useful, iPS-derived hepatocytes should recapitulate the diversity of metabolic phenotypes observed in human populations (Li 2008), express drug-metabolizing enzymes at equivalent levels to primary human hepatocytes, and maintain stable functionality in vitro (Sartipy and Björquist 2011). The Cellartis iPS Cell to Hepatocyte Differentiation System addresses all these challenges and allows for the simple generation of mature hepatocytes in vitro.

Results  

A simple and universal system for differentiation of human iPS cells into hepatocytes

The Cellartis Cell iPS to Hepatocyte Differentiation System is a complete system that includes all media, supplements, and a protocol to differentiate any iPS cell line into hepatocytes (Laydon, Bangham, and Asquith 2015). The protocol mimics liver development in vivo: iPS cells are first differentiated into definitive endoderm (DE) cells and are then further differentiated into mature hepatocytes. Differentiated mature hepatocytes are ready for your research applications at differentiation day 21, and have an experimental time window of at least 11 days.

iPS Cell to Hepatocyte Differentiation workflow

iPS Cell to Hepatocyte Differentiation System Overview. The complete system includes media, supplements, and a protocol to differentiate any iPS cell line into hepatocytes.

A homogeneous culture of highly pluripotent iPS cells is an essential starting point for efficient and reproducible hepatocyte differentiation. We have developed an optimized iPS cell culture system, the Cellartis DEF-CS Culture System, which is included in the Cellartis iPS to Hepatocyte Differentiation System. DEF-CS enables monolayer culture of iPS cells that are highly pluripotent and exhibit low spontaneous differentiation and stable karyotypes, thereby providing iPS cells that are highly amenable to downstream differentiation (Laydon, Bangham, and Asquith 2015).

Undifferentiated iPS cells culture/DEF-CS system

Culture of undifferentiated iPS cells in the Cellartis DEF-CS Culture System. Panel A. DEF-CS facilitates the expansion of iPS cell line ChiPSC4 in a non-colony, 2D monolayer. Panel B. In iPS cell line ChiPSC18, the karyotype remains stable through 20 passages when cultured in DEF-CS. Panel C. iPS cells cultured in DEF-CS highly express pluripotency markers OCT4 (green, top panel) and SSEA-4 (green, bottom panel). Nuclei are stained with DAPI (blue). Panel D. Multiple iPS cell lines exhibit robust cell growth and a uniform doubling time in DEF-CS. Data adapted from Asplund et al. 2016 (Laydon, Bangham, and Asquith 2015).

Using a protocol that combines media, supplements, and coating reagents optimized for 2D monolayer culture, the pluripotent stem cells are then differentiated into definitive endoderm, which is an essential embryonic mid-stage for all endodermal cell types (Zaret and Grompe 2008). This process takes 7 days, during which the pluripotency transcription factor OCT4 is downregulated, and several markers of DE cells (SOX17, FOXA2, CXCR4, and HHEX) are upregulated (Laydon, Bangham, and Asquith 2015). DE cells are then passaged, re-plated, and prompted to become hepatocytes. Functional hepatocytes are available for experimentation within three weeks.

Homogeneous definitive endoderm cell derivation

Derivation of homogenous definitive endoderm cells from hiPS cell lines. Panel A. RT-qPCR analysis indicates down-regulation of the expression of stem cell markers OCT4 (A1) and NANOG (A2) in DE cells. In contrast, mRNA expression of DE markers CXCR4 (A3) and SOX17 (A4) is strongly up-regulated in DE cells compared to undifferentiated hiPS cells. Low expression of the extraembryonic marker SOX7 (A5) indicates minimal occurrence of extraembryonic endoderm cells. Panel B. Immunocytochemical staining for the DE marker SOX17 (B2, B5) and the stem cell marker OCT4 (B3, B6) reveal minimal presence of OCT4 immunopositive nuclei and a majority of SOX17 immunopositive nuclei in DE cells derived from hiPS cell lines ChiPSC6b and P11025. Nuclei are stained with DAPI (B1, B4).

iPS cell-derived hepatocytes resemble primary hepatocytes and express mature hepatocyte markers

To evaluate the robustness and applicability of our protocol, we tested hepatic differentiation from 20 different iPS cell lines. Hepatocytes generated using this system morphologically resemble primary hepatocytes in that they are polygonal cells, bi-nucleated, and have distinct cell borders. We also tested for expression of mature hepatocyte markers and loss of the OCT4 transcription factor. Hepatocytes derived from all 20 iPS cell sources were immunopositive for the transcription factor HNFα, which is a master transcription factor responsible for hepatocyte fate specification. The vast majority—93.8% ± 0.7 (mean ± SEM) of DAPI-stained hepatocyte nuclei—also stained positive for HNFα, and no OCT4-positive cells were observed, indicating the absence of any residual pluripotent cells (Laydon, Bangham, and Asquith 2015).

iPS cell donor-dervied hepatocyte morphology

Hepatocytes derived from multiple iPS cell donors exhibit expected morphology. Panel A. 20 different iPS cell lines representing 19 different donors are successfully differentiated using the iPS Cell to Hepatocyte Differentiation System. Representative images of 16 lines, taken 21–25 days after the start of differentiation, show that the iPS cell-derived hepatocytes morphologically resemble primary hepatocytes and exhibit consistent differentiation patterns. Panel B. At differentiation day 28, iPS cell-derived hepatocytes are immunostained with HNF4α and nuclei are stained with DAPI. On average, 93.8% of cells are HNF4α-positive, as indicated by the horizontal line.

We also tested for expression of fetal and mature hepatocyte markers during differentiation into hepatocytes using RT-qPCR. Interestingly, the levels for the fetal hepatic marker AFP are highest at day 21 and gradually decrease as the hepatocytes mature. The opposite is true for the adult hepatocyte markers albumin, PXR, CYP1A2, CYP2C9 and CYP3A4, which are gradually upregulated in the maturing hepatocytes. CYP1A2, 2C9, and 3A4 are important drug metabolizing enzymes detected in adult liver tissue but not in fetal liver tissue. Taken together, these data indicate a homogeneous population of mature hepatocytes derived from multiple iPS cell lines.

Human iPS hepatocytes human metabolic diversity

iPS cell-derived hepatocytes reflect maturing hepatocyte mRNA expression profiles. mRNA expression of several hepatocyte markers was analyzed between day 11 and 42 after the start of differentiation using RT-qPCR. mRNA levels were normalized to the reference gene CREBBP. The levels for the fetal hepatocyte marker AFP are highest at day 21 and gradually decrease as the hepatocytes mature, while the opposite is true for the adult hepatocyte markers albumin, PXR, CYP1A2, CYP2C9, and CYP3A4, which are gradually upregulated in the maturing hepatocytes.

iPS cell-derived hepatocytes exhibit metabolic activity and reflect metabolic diversity

Drug metabolism is a central hepatocyte function. A critical metric for terminal hepatocyte differentiation is the expression and activity of drug metabolizing enzymes in the cytochrome P450 (CYP) family. Therefore, in addition to RT-qPCR analysis of the enzymes CYP1A2, CYP2C9 and CYP3A4 shown above, we measured the activities of key CYP enzymes by LC/MS. We detected CYP1A, CYP3A, CYP2C9 and CYP2C19 activity at levels comparable to human primary hepatocytes cultured for 20 hr post-thawing (cryo hpep data, below). Interestingly, we observed variation in CYP activities (Phase I metabolism) between hepatocytes derived from different iPS cell lines or from different primary hepatocytes. We then determined if the foregoing variation was due to inter-experimental variation or differences in the donor source. Hepatocytes were repeatedly derived from ChiPSC18 (n=4), and CYP activity assays were performed on the different batches. We observed very small batch-to-batch variation in CYP activity (ChiPSC18 data, below). These data strongly indicate that the variability in CYP activity between cell lines was not due to an artifact of the differentiation protocol, but instead are representative of genuine phenotypic variation in iPS cell-derived hepatocytes from various human sources.

Human iPS cell-derived hepatocytes CYP activity

CYP activity of human iPS cell-derived hepatocytes recapitulates the inter-individual variation of the human population. CYP activity was measured by LC/MS and normalized to the protein content per well in iPS cell-derived hepatocytes (29 days after the start of differentiation). Activities were comparable with cryopreserved human hepatocytes (cryo hphep) from four different donors. Hepatocytes derived from five different hiPS cell lines show diverse CYP activity profiles, reflecting the metabolic diversity found in human primary hepatocytes from different donors. For example, CYP2C19 activity is low in ChiPSC18, but high in ChiPSC6b, reflecting naturally occurring inter-individual variation.

Conclusions  

The robust and reproducible generation of hepatocytes from iPS cells has enormous implications for basic and clinical research, drug development, and cell-based therapy. The Cellartis iPS to Hepatocyte Differentiation System is a complete system with a universal differentiation protocol to make hepatocytes from various iPS cell lines. It includes the DEF-CS media for maintenance of iPS cells in monolayer culture in order to generate optimal starting material with low spontaneous differentiation and high pluripotency for subsequent differentiation into DE cells and hepatocytes. Our system allows for directed differentiation of 20 different iPS cells resulting in cells with a clear hepatocyte morphology with no residual pluripotent cells. The iPS-derived hepatocytes express markers typical of mature hepatocytes such as HNFα, albumin, PXR, and Phase I metabolizing enzymes. Finally, the iPS cell-derived hepatocytes faithfully recapitulate the metabolic diversity found in the human population.

The Cellartis iPS to Hepatocyte Differentiation System has powerful utility for a breadth of applications from basic liver biology research, disease modeling, hepatitis studies, and pre-clinical work, to cell-based therapy for chronic liver diseases. Importantly, hepatocytes made using this system reflect the metabolic diversity inherent to human cells. Therefore, the Cellartis iPS to Hepatocyte Differentiation System will allow a researcher to make panels of iPS cell-derived human hepatocytes to interrogate potential patient-specific phenotypes. Safety and toxicology labs interested in developing in-house hepatocyte generation methods would also be interested in such cellular panels. In all, the Cellartis iPS to Hepatocyte Differentiation System greatly simplifies the generation of hepatocytes from various iPS cells, providing any customer a complete solution for their research.

Methods  

Differentiation of iPS cells into hepatocytes

The differentiation protocol is as published by Asplund et al. 2016 with some modification to the maturation stage (Laydon, Bangham, and Asquith 2015). Briefly, iPS cells are cultured in DEF-CS at a defined cell density in day 0 cell culture medium. Medium changes over the next seven days yield homogenous DE cells that are then enzymatically dissociated and re-plated for further hepatocyte differentiation. The hepatocyte differentiation media guides the DE cells through the same developmental stages that occur during liver development in vivo: ventral foregut, hepatoblast, fetal-like hepatocyte and mature hepatocyte. This process takes another two weeks. At the end of this process, hepatocyte maintenance media provided allows for maintenance of function for at least another 11 days.

Immunocytochemistry

Cells were stained with primary anti-HNF4α antibody (Santa Cruz Biotechnology) followed by donkey anti-rabbit secondary antibody and counterstained with DAPI (Laydon, Bangham, and Asquith 2015). Representative HNFα pictures were merged with DAPI, and the number of HNFα-DAPI double-positive nuclei was evaluated.

RT-qPCR

Analyses were performed using OCT4 (Hs01654807_s1), NANOG-1 (Hs02387400_g1), SOX17(Hs00751752_s1), CXCR4(Hs00237052_m1), SOX7(Hs00846731_s1), CREBBP (Hs00231733_m1), AFP (Hs00173490_m1), Albumin (Hs00910225_m1), CYP1A2 (Hs01070374_m1), CYP2C9 (Hs004260376_m1), and CYP3A4 (Hs00604506_m1) TaqMan probes (Applied Biosystems). Expression levels were calculated using the ΔΔCt method, normalized to CREBBP expression.

CYP activity assay

Cells were washed twice with warm WME without phenol red (Thermo Fisher Scientific). 26 µM phenacetin (CYP1A), 50 µM mephenytoin (CYP2C19), 9 µM diclofenac (CYP2C9) and 3 µM midazolam (CYP3A) were added to the plates in warm WME supplemented with 0.1% PEST, 25 mM HEPES, and 2 mM L-glutamine. After 2h, 100 µl of supernatant was collected and stored at –80°C until LC/MS analysis of paracetamol, hydroxy-mephenytoin, hydroxy-diclofenac, and hydroxy-midazolam was performed. Protein amount per well was quantified by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Metabolite concentrations were normalized to protein amount per well and assay duration.

References  

Asplund, A. et al. One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev. Reports 12, 90-104 (2016).

Behbahan, I. S. et al. New Approaches in the Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Hepatocytes. Stem Cell Rev. Reports 7, 748-759 (2011).

Kajiwara, M. et al. Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells. Proc. Natl. Acad. Sci. U. S. A. 109, 12538-43 (2012).

Laydon, D. J., Bangham, C. R. M. & Asquith, B. Estimating T-cell repertoire diversity: limitations of classical estimators and a new approach. Philos. Trans. R. Soc. B Biol. Sci. 370, 20140291 (2015).

Li, A. P. Human hepatocytes as an effective alternative experimental system for the evaluation of human drug properties: general concepts and assay procedures. ALTEX 25, 33-42 (2008).

Sartipy, P. & Björquist, P. Concise Review: Human Pluripotent Stem Cell-Based Models for Cardiac and Hepatic Toxicity Assessment. Stem Cells 29, 744-748 (2011).

Zaret, K. S. & Grompe, M. Generation and Regeneration of Cells of the Liver and Pancreas. Science 322, 1490-1494 (2008).      

Related products

Cat. # Product Size Price License Quantity Details
Y30055 Cellartis® iPS Cell to Hepatocyte Differentiation System 1 Kit USD $1512.00

License Statement

ID Number  
C007 This product is manufactured and sold by Takara Bio Inc. and/or its affiliate(s) (“Takara”) under a license from ViaCyte Inc. (“ViaCyte”). This product is covered by one or more claims of patents owned by ViaCyte, including WO2005063971, WO2006017134, WO2006071911, WO2005116073 and WO200616999, and their foreign counterparts. The use of this product is strictly limited to purchaser's own internal research. Purchaser has no right to use this product or its components in humans for any purposes including but not limited to diagnostics, therapeutics, or clinical trials. Purchaser has no right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact Takara. No express or implied license is granted to the purchaser.
C001 This product is manufactured and sold by Takara Bio Europe SAS based on a commercial license to certain intellectual property rights held by Wisconsin Alumni Research Foundation (“WARF”). This product is covered by one or more claims of U.S. Patent No. 7,514,260 and its foreign counterparts. The purchase of this product conveys to the buyer the non-transferable right to use the product for its intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us.

The Cellartis iPS Cell to Hepatocyte Differentiation System is a complete system for differentiation of human induced pluripotent stem (iPS) cells to hepatocytes via definitive endoderm (DE). This kit includes the Cellartis DEF-CS Culture System for expansion of undifferentiated iPS cells, the Cellartis Definitive Endoderm Differentiation Kit for differentiation to DE cells, and the Cellartis Hepatocyte Differentiation Kit for subsequent differentiation to hepatocytes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Immunocytochemistry analysis of hepatocyte differentiation

Immunocytochemistry analysis of hepatocyte differentiation
Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

logo strip white

©2025 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Immune profiling
  • Real-time PCR
  • Great value master mixes
  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
  • Stem cell research
  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Automated platforms
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • OEM
  • Portfolio
  • Process
  • Facilities
  • Request samples
  • FAQs
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip ND system services
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • Shasta Single Cell System introduction
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • RNA-seq
  • Technical notes
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Spatial biology
  • Real-time PCR
  • Download qPCR resources
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • mRNA and cDNA synthesis
  • mRNA synthesis
  • cDNA synthesis
  • PCR
  • Citations
  • PCR selection guide
  • Technical notes
  • FAQ
  • Cloning
  • Automated In-Fusion Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Contact us
  • mRNA and protein therapeutics
  • Characterizing the viral genome and host response
  • Identifying and cloning protein targets
  • Expressing and purifying protein targets
  • Immunizing mice and optimizing vaccines
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Kickstart your cancer research with long-read sequencing
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer transcriptome analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Embgenix FAQs
  • Preimplantation genetic testing
  • ESM partnership program
  • ESM Collection Kit forms
  • Infectious diseases
  • Develop vaccines for HIV
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Our history
  • In the news
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • HQ-grade reagents
  • International Contacts by Region
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop exceptional products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
Takara Bio
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Immune profiling
  • Real-time PCR
  • Great value master mixes
  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
  • Stem cell research
  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Automated platforms
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Services & Support
  • OEM
  • Instrument services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Portfolio
  • Process
  • Facilities
  • Request samples
  • FAQs
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip ND system services
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Spatial biology
  • Real-time PCR
  • Nucleic acid purification
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • Shasta Single Cell System introduction
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • RNA-seq
  • Technical notes
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Download qPCR resources
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • mRNA and cDNA synthesis
  • mRNA synthesis
  • cDNA synthesis
  • PCR
  • Citations
  • PCR selection guide
  • Technical notes
  • FAQ
  • Cloning
  • Automated In-Fusion Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • mRNA and protein therapeutics
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Infectious diseases
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Contact us
  • mRNA and protein therapeutics
  • Characterizing the viral genome and host response
  • Identifying and cloning protein targets
  • Expressing and purifying protein targets
  • Immunizing mice and optimizing vaccines
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Kickstart your cancer research with long-read sequencing
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer transcriptome analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Embgenix FAQs
  • Preimplantation genetic testing
  • ESM partnership program
  • ESM Collection Kit forms
  • Infectious diseases
  • Develop vaccines for HIV
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • In the news
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • HQ-grade reagents
  • International Contacts by Region
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • International Contacts by Region
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors
  • Need help?
  • Privacy request
Takara Bio
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us