Gene editing in hiPS cells
At Takara Bio, we have expertise in both human pluripotent stem cell culture and gene editing. With over 15 years of stem cell experience, we offer products like the DEF-CS culture system, which you can rely on for high survival of your single-cell clones, stable karyotype, and maintenance of pluripotency—all of which are essential for downstream applications using edited hiPS cells. Our gene editing and single-cell cloning experts have developed powerful gene editing and screening tools as well as reliable workflows to successfully and efficiently edit hiPS cells.
Explore our technical notes and case studies to learn more about our technologies.
Tagging an endogenous gene with AcGFP1 in hiPS cells
Workflow for tagging endogenous genes to generate reporter lines.
Tagging an endogenous gene with a myc tag in hiPS cells
Workflow for tagging endogenous genes with an epitope tag.
Knocking out an endogenous gene (CD81) in hiPS cells
Workflow for knocking out endogenous genes while maintaining pluripotency and stable karyotype.
Introducing a tyrosinemia-related SNP in hiPS cells
Workflow for knocking in disease-related SNPs.
Inserting an expression cassette into the AAVS1 locus in hiPS cells
Workflow for knocking in an expression cassette into a safe-harbor locus.
Editing hiPS cells using electroporation
Workflow for generating edited, clonal hiPS cell lines using electroporation-based delivery of editing machinery.
Editing hiPS cells using gesicle technology
Workflow for generating edited, clonal hiPS cell lines using gesicle-based delivery of editing machinery.
Single-cell cloning of hiPS cells
Workflow for establishing robust, clonal hiPS cell lines.
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