Differentiation into cells of neural lineage is a challenging and time-consuming process. Conventional differentiation media initially provided a baseline culture system for generating neural cells. However, these media were relatively inefficient, giving low percentages of neural cells, and cumbersome, in that the type of medium used for differentiation had to be changed completely before expansion of intermediate neural stem (NS) cells. Furthermore, these media were often specific only to mouse cells, necessitating the development of a medium compatible with human cells.
RHB-A medium is the next-generation formulation of N2B27-based medium for NS cell culture and neural differentiation. This optimized medium was developed to both improve the differentiation of human pluripotent stem cells into neurons, while also enabling the maintenance and expansion of NS cells upon supplementation with epidermal growth factor (EGF) and fibroblast growth factor-basic (FGF-basic).
In the following experiments, RHB-A medium was directly compared to different vendors' media for the expansion of human NS cells and was further tested for the differentiation of those expanded cells into both neurons and astrocytes.