Pluripotent stem cells
At Takara Bio, we are experts in human pluripotent stem cell culture. We developed the Cellartis DEF-CS 500 Culture System, an easy-to-use, complete culture system for efficient iPS cell expansion in a feeder-free, defined environment. With this system, cells are maintained in an undifferentiated state with virtually no background spontaneous differentiation. The DEF-CS culture system allows for easy culture of iPS cells as a non-colony type (2D) monolayer. The highly reproducible nature of the system, coupled with its ability to ensure an efficient and predictable growth rate, makes the DEF-CS culture system ideal for the expansion and scale-up of a homogeneous population of iPS cells.
Explore our technical notes to learn more about the benefits of the DEF-CS 500 Culture System and the applications it supports.
Using the DEF-CS system to culture human iPS cells
Learn how human pluripotent stem cells are cultured in the DEF-CS culture system.
Direct comparison of the Cellartis DEF-CS system with different vendors' systems for culturing human pluripotent stem cells
Learn how the DEF-CS culture system outperforms other culture systems.
Comparison of the Cellartis DEF-CS system with other vendors' human iPS cell culture systems
Single-cell cloning of hiPS cells
Workflow for establishing robust, clonal hiPS cell lines.
Reprogramming of human PBMCs using Sendai virus and the Cellartis DEF-CS 500 Culture System
View data on reprogramming PBMCs to iPSCs with a high success rate.
Reprogramming of human fibroblasts using Sendai virus and the Cellartis DEF-CS 500 Culture System
View data on reprogramming fibroblasts to iPSCs with a high success rate.
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