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Technical note icon Beta cells for disease modeling technical note
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Technical note icon Beta cells for disease modeling technical note

Pancreatic beta cells derived from human induced pluripotent stem cells

hiPSC-derived beta cells

Cellartis hiPS beta cells have been differentiated from our ChiPSC12 and ChiPSC22 lines of human induced pluripotent stem (hiPS) cells, dissociated into a single-cell suspension, and frozen in vials. Each kit contains beta cells, coating substrate, basal media, and a supplement.

Cellartis hiPS beta cells have been differentiated from our ChiPSC12 and ChiPSC22 lines of control human induced pluripotent stem (hiPS) cells, dissociated into a single-cell suspension, and frozen in vials. Each kit contains beta cells, coating substrate, basal media, and a supplement.

Cellartis hiPS cell-derived beta cell kits contain beta cells generated in vitro using a standardized protocol that mimics embryonic development. These cells secrete high amounts of insulin and C-peptide, and they show substantial protein expression of insulin/C-peptide, MAFA, NKX6-1, PDX1, and UCN3. We offer two complete kits, each containing cells that originate from healthy human donors, including one with a diabetes-susceptible HLA type (HLA-A*02:01). The kits are ideal for investigating beta cell function, diabetes/disease modeling, and compound screening for insulin secretion and regulation.

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Cat. # Product Size License Quantity Details
Y10100 Cellartis® hiPS Beta Cells (from ChiPSC12) Kit 1 Kit USD $1782.00

License Statement

ID Number  
C005 This product is manufactured and sold by Takara Bio Europe AB based on a license to Cellectis SA. The use of this product is strictly limited to purchaser’s own internal research, including basic research, drug discovery research up to and including IND-enabling preclinical toxicological studies or equivalents thereof, excluding any use in the T-cell field. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us. No express or implied license is granted to the purchaser.
L67 This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 9,938,585 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This Product is manufactured and sold by Takara Bio Inc. and/or its affiliate(s) based on a patent license from iPS Academia Japan, Inc.

No express or implied license is granted to the purchaser.

"Product" means the differentiated cells manufactured and sold by Takara Bio Inc.

"Internal research purpose" means the use of this Product as a tool of internal research for the sole benefit of the purchaser or purchaser's contract research customer, (including screening of compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use), provided, however, that the following activities using this Product are strictly prohibited and expressly excluded from Internal research purposes:

With the exemption for academic and non-commercial clinical research done by non-profit institutions;

1) Administration of this Product to humans.

2) Use of this Product for the diagnostic testing, prevention, or treatment of human and/or animal diseases.

3) Use of this Product in studies involving administration to humans for cell therapy, regenerative medicine etc.

Purchaser may use this Product for its own Internal research purposes.

Purchaser shall not use the Products for its commercial purposes.

Notwithstanding the foregoing, the purchaser of this Product may provide third parties with contract research services by using this Product only for such third party's Internal research purposes.

Cellartis hiPS Beta Cells (from ChiPSC12), derived from human induced pluripotent stem cells, are ideal for diabetes modeling and drug discovery.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Donor information and HLA typification data.

Donor information and HLA typification data.
Donor information and HLA typification data. The complete kits each contain cells that originate from a different donor/HLA type. ChiPSC12 was sourced from skin fibroblasts from a healthy human 24-year-old adult male volunteer (European/North African; 77 kg/177 cm). ChiPSC22 was sourced from skin fibroblasts from a healthy human 32-year-old adult male volunteer (European/North African; 74 kg/179 cm). ChiPSC22-derived beta cells originate from a human donor with a diabetes-susceptible HLA type, HLA-A*02:01. Beta cells from ChiPSC22 can be used alone or in combination with beta cells from ChiPSC12, which have an alternate HLA type.

Back

Cellartis hiPS Beta Cells (from ChiPSC12) were exposed to incretin (100 nM GLP-1 or 100 nM exenatide) for 45 min in 5.5 mM glucose.

Cellartis hiPS Beta Cells (from ChiPSC12) were exposed to incretin (100 nM GLP-1 or 100 nM exenatide) for 45 min in 5.5 mM glucose.
Cellartis hiPS Beta Cells (from ChiPSC12) were exposed to incretin (100 nM GLP-1 or 100 nM exenatide) for 45 min in 5.5 mM glucose. The content of secreted C-peptide was analyzed using the Mercodia C-peptide ELISA kit. The results are mean � SEM (n=5, GLP-1; n=4, exenatide). *p<0.05.

Back

Human iPS cell-derived beta cells synthesize and secret insulin.

Human iPS cell-derived beta cells synthesize and secret insulin.
Human iPS cell-derived beta cells synthesize and secret insulin. Staining for C-peptide and insulin was performed by immunofluorescence on beta cells at Day 14 post-thawing. Colocalized insulin and C-peptide indicate intracellular insulin synthesis. Scale bar = 100 �m.

Back

Cellartis beta cells were fixed 14 days post-thaw and co-stained for C-peptide (green) and MAFA (red).

Cellartis beta cells were fixed 14 days post-thaw and co-stained for C-peptide (green) and MAFA (red).
Cellartis beta cells were fixed 14 days post-thaw and co-stained for C-peptide (green) and MAFA (red).
Y10106 Cellartis® hiPS Beta Cells (from ChiPSC22) Kit 1 Kit USD $1782.00

License Statement

ID Number  
C005 This product is manufactured and sold by Takara Bio Europe AB based on a license to Cellectis SA. The use of this product is strictly limited to purchaser’s own internal research, including basic research, drug discovery research up to and including IND-enabling preclinical toxicological studies or equivalents thereof, excluding any use in the T-cell field. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us. No express or implied license is granted to the purchaser.
L67 This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 9,938,585 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This Product is manufactured and sold by Takara Bio Inc. and/or its affiliate(s) based on a patent license from iPS Academia Japan, Inc.

No express or implied license is granted to the purchaser.

"Product" means the differentiated cells manufactured and sold by Takara Bio Inc.

"Internal research purpose" means the use of this Product as a tool of internal research for the sole benefit of the purchaser or purchaser's contract research customer, (including screening of compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use), provided, however, that the following activities using this Product are strictly prohibited and expressly excluded from Internal research purposes:

With the exemption for academic and non-commercial clinical research done by non-profit institutions;

1) Administration of this Product to humans.

2) Use of this Product for the diagnostic testing, prevention, or treatment of human and/or animal diseases.

3) Use of this Product in studies involving administration to humans for cell therapy, regenerative medicine etc.

Purchaser may use this Product for its own Internal research purposes.

Purchaser shall not use the Products for its commercial purposes.

Notwithstanding the foregoing, the purchaser of this Product may provide third parties with contract research services by using this Product only for such third party's Internal research purposes.

Cellartis hiPS Beta Cells (from ChiPSC22), derived from human induced pluripotent stem cells, are ideal for diabetes modeling and drug discovery.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Donor information and HLA typification data.

Donor information and HLA typification data.
Donor information and HLA typification data. The complete kits each contain cells that originate from a different donor/HLA type. ChiPSC12 was sourced from skin fibroblasts from a healthy human 24-year-old adult male volunteer (European/North African; 77 kg/177 cm). ChiPSC22 was sourced from skin fibroblasts from a healthy human 32-year-old adult male volunteer (European/North African; 74 kg/179 cm). ChiPSC22-derived beta cells originate from a human donor with a diabetes-susceptible HLA type, HLA-A*02:01. Beta cells from ChiPSC22 can be used alone or in combination with beta cells from ChiPSC12, which have an alternate HLA type.

Back

Human iPS cell-derived beta cells synthesize and secret insulin.

Human iPS cell-derived beta cells synthesize and secret insulin.
Human iPS cell-derived beta cells synthesize and secret insulin. Staining for C-peptide and insulin was performed by immunofluorescence on beta cells at Day 14 post-thawing. Colocalized insulin and C-peptide indicate intracellular insulin synthesis. Scale bar = 100 �m.

Back

Cellartis beta cells were fixed 14 days post-thaw and co-stained for C-peptide (green) and MAFA (red).

Cellartis beta cells were fixed 14 days post-thaw and co-stained for C-peptide (green) and MAFA (red).
Cellartis beta cells were fixed 14 days post-thaw and co-stained for C-peptide (green) and MAFA (red).
Y10108 Cellartis® hiPS Beta Cell Media Kit 1 Kit USD $1027.00

The Cellartis hiPS Beta Cell Media Kit is a complete system for the maintenance of beta cells provided within the Cellartis hiPS Directed Differentiation to Beta Cells Services (Cat. Nos. Y60230, Y60232, and Y60234). One Cellartis hiPS Beta Cell Media Kit is enough to culture one vial of beta cells. The Cellartis hiPS Beta Cell Media Kit contains a supplement, a coating substrate, and two types of basal media.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Overview

  • Beta cells from ChiPSC12 and ChiPSC22 originate from healthy human donors
  • Beta cells from ChiPSC22 originate from a donor with a diabetes-susceptible HLA type, HLA-A*02:01 (Marron et al. 2002)
  • Beta cells from ChiPSC22 can be used alone or in combination with beta cells from ChiPSC12, which have an alternate HLA type
  • Cells show protein expression of insulin/C-peptide, MAFA, NKX6-1, PDX1, and UCN3
  • Cells demonstrate insulin and C-peptide secretion
  • Cells were derived with a protocol that mimics embryonic development
  • Cells provide reproducible and biologically relevant data

More Information

Donor information

The tissues used for the reprogramming of somatic cells into hiPSCs are from donors who have signed informed consent which outlines in detail the purpose of the donation and the procedure for processing of the donated tissue. In order to protect the privacy and the confidentiality of the donors, all identifiers associated with the donors have been removed. The donor consent was obtained for commercial use. The donation did not result in any financial gain for the donors.

  • ChiPSC12 was sourced from skin fibroblasts from a healthy 24-year-old European/North African male volunteer (77 kg/177 cm).
  • ChiPSC22 was sourced from skin fibroblasts from a healthy 32-year-old European/North African male volunteer (74 kg/179 cm) with a diabetes-susceptible HLA type, HLA-A*02:01.

HLA typification data for each line is shown below.

Cellartis hiPS Beta Cells (from ChiPSC12) (Cat. # Y10100) Cellartis hiPS Beta Cells (from ChiPSC22) (Cat. # Y10106)
HLA-A*01:01 HLA-A*02:01
HLA-B*08:01, HLA-B*37:01 HLA-B*07:02, HLA-B*40:01
HLA-C*06:02, HLA-C*07:01 HLA-C*03:04, HLA-C*07:02
HLA-DRB1*03:01, HLA-DRB1*11:04 HLA-DRB1*13:02, HLA-DRB1*14:01
HLA-DQB1*02:01, HLA-DQB1*03:01 HLA-DQB1*05:03, HLA-DQB1*06:04
HLA-DPB1*01:01, HLA-DPB1*04:01 HLA-DPB1*03:01, HLA-DPB1*04:01

Applications

  • Disease modeling for diabetes
  • Compound screening for insulin secretion and regulation
  • Beta-cell functionality
  • GSIS (glucose-stimulated insulin secretion) analysis
  • Incretin response studies
  • Autoimmune beta-cell destruction
  • Pancreatitis and beta-cell function
  • Transdifferentiation

Components

  • 1 vial (4.8 x 106 viable cells) of Cellartis hiPS beta cells from ChiPSC12 or ChiPSC22
  • 11 vials (360 µl/vial) of Cellartis Beta Cell Supplement
  • 1 tube (4 ml) of Cellartis Beta Cell Coating
  • 1 bottle (160 ml) of Cellartis Beta Cell Basal Medium
  • 1 bottle (30 ml) of Cellartis Beta Cell Basal Medium 2

References

Marron, M. et al. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Nat Acad Sci U. S. A. 99, 13753–8 (2002).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Cellartis human iPS cell-derived beta cells for diabetes drug discovery and disease modeling

Studying diabetes and metabolic disorders using hiPSC-derived beta cells.

Read the technical note

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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