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  • Direct SARS-CoV-2 detection from saliva
  • Viral detection via qPCR
  • Direct viral detection via qPCR
  • High-throughput viral detection via qPCR
SARS-CoV-2 image Direct viral detection via qPCR
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COVID-19 research

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  • Viral detection with qPCR
    • Direct SARS-CoV-2 detection from saliva
    • Viral detection via qPCR
    • Direct viral detection via qPCR
    • High-throughput viral detection via qPCR
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SARS-CoV-2 image Direct viral detection via qPCR
SmartChip system dispensing reagents High-throughput viral detection via qPCR
processing respiratory samples Processing respiratory samples
Wastewater Webinar: Resistomap tracks AR and SARS-CoV-2 in wastewater

Real-time qPCR for COVID-19 research

The recent emergence of the coronavirus SARS-CoV-2 (the virus that causes COVID-19) has led to a need for rapid and reliable detection. Takara Bio offers solutions to support researchers and clinicians studying this novel coronavirus, such as viral RNA purification kits, NGS kits, and RT-qPCR kits that can be used with established primers for sensitive detection of SARS-CoV-2 down to five viral copies (Figure 1).

Takara Bio reagents in FDA Emergency Use Authorizations 

Our high-quality reagents, manufactured under ISO 13485:2016 conditions, have been utilized in multiple Emergency Use Authorizations issued by the Federal Drug Administration (FDA) for COVID-19 testing:

  • Ipsum Diagnostics, LLC
  • Hackensack University Medical Center
  • LifeHope Laboratory

COVID-19 detection using PrimeDirect RT-qPCR

Figure 1. Reliable detection of SARS-CoV-2 at 5, 50, 500, 5,000, and 50,000 copies with our one-step RT-qPCR kits. These data were generated by Takara Bio using publicly available protocols from the CDC. These data have not been validated by the CDC.


Publications: highly sensitive, fast SARS-CoV-2 detection with PrimeScript III one-step RT-qPCR

In an April, 2020 bioRxiv paper, Brown et al., from the Great Ormond Street Hospital (NHS, London, UK), compared One Step PrimeScript III RT-qPCR Mix to QuantiFast Multiplex PCR +R master mix (Qiagen), TaqMan Fast Virus 1-Step Master Mix (Thermo), and TaqPath One Step RT-qPCR Master Mix (Thermo) in order to test compatibility and sensitivity for SARS-CoV-2 detection.  

In the initial experiment to test the limit of detection, One Step PrimeScript III RT-qPCR Mix showed superior sensitivity by consistently detecting 1 viral copy/µl with all three primer/probe assays. The authors showed that the lower limit of detection for this kit is 10 to 100 times greater than the competitors' solutions.

Next, the authors tested 74 real-life samples from nose and/or throat swabs from patients with suspected cases of SARS-CoV-2 infection. They compared PrimeScript III mix to the Quantifast multiplex kit (Qiagen). Of the 74 swab samples tested, 67/74 showed concordant results between both master mixes. However, among the discordant results, PrimeScript III mix could detect 7 patient samples with low RNA sample inputs (median Ct = 40.9 [range 38.5–41.9]), whereas these samples were undetected by Quantifast, showing that the increased sensitivity of PrimeScript III one-step RT-qPCR reduces the number of false negatives.

Furthermore, the authors compared the overall reaction speed for all mixes. The PrimeScript III one-step RT-qPCR experiment can be run in less than 1 hour (33% faster than Quantifast Multiplex PCR +R master mix), meaning even faster detection of SARS-CoV-2 infection. Faster reaction speeds will help increase the throughput for COVID-19 diagnostic labs.

Brown et al. conclusively showed that the PrimeScript III One Step RT-qPCR Mix outperforms other one-step RT-qPCR solutions in both sensitivity and speed. In a time where screening for SARS-CoV-2 infection is of utmost importance, the efficiency and rapidity of One Step PrimeScript III RT-qPCR Mix plays a critical role in the optimal management and control of this pandemic.

In a July, 2020 medRxiv paper, Reijns et al., from the University of Edinburgh, also found that One-Step PrimeScript III RT-qPCR Mix demonstrated superior sensitivity when performing a multiplex reaction on patient samples. Critically, they report that this mix was able to detect positive samples that were previously found negative using the TaqPath COVID-19 Combo Kit. Moreover, they reported that the assay developed using the Takara Bio mix was 1/10th the price of the other kits. These data reinforce the exceptional performance and value of our mixes for SARS-CoV-2 detection.


Compatibility with primer/probe sets from published SARS-CoV-2 detection protocols

We tested qPCR detection of SARS-CoV-2 using the One Step PrimeScript III RT-PCR Kit and PrimeDirect Probe RT-qPCR Mix in publicly available protocols with primer sets from the US and China CDCs, WHO, and Japan NIID. Sensitivity of the assay varies depending on the nature of the template. The template used in the Japan NIID protocol was provided by the NIID, and the templates used in the other protocols were synthesized RNA prepared by Takara Bio. You can download our results by clicking on the PDF thumbnail images below.

The China CDC has also published a paper using our kits for SARS-CoV-2 detection (Zhu et al. 2019).

US CDC

Flyer thumbnail

WHO

Flyer thumbnail

China CDC

Flyer thumbnail

Japan NIID

Flyer thumbnail

The results were generated by Takara Bio by following the publicly available protocols. Use of our products in these protocols has not been validated by external agencies nor optimized by our R&D team. These data are provided for illustrative purposes only. Please refer to published literature for more information about these protocols.


We have bulk formats of our one-step RT-qPCR kits. If you are interested, please contact us with your inquiry.


Explore industry-leading products that can advance your COVID-19 research:

Cat. # Product Size Price License Quantity Details
RR600A One Step PrimeScript™ III RT-PCR Kit 200 Rxns USD $342.00

One Step PrimeScript III RT-qPCR Mix is a dedicated reagent for one-step real-time, probe-based RT-qPCR (using the 5’ nuclease method). This 2X premix does not freeze at its storage temperature of –20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube. The reverse transcription reaction uses the novel PrimeScript III RTase, which displays increased heat tolerance (up to 55℃) while maintaining the specificity and extensibility of PrimeScript RTase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis, TaKaRa Taq HS performs highly specific and efficient PCR amplification, while the fluorescence emitted by the probe is detected in real-time. One Step PrimeScript III RT-qPCR Mix is also highly resistant to a wide variety of inhibitory substances in blood and soil, allowing stable one-step real-time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc.

Cat. # RR600A contains sufficient reagent for performing 200 reactions in a volume of 25 µl per reaction.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR600A: One Step PrimeScript III RT-PCR Kit

RR600A: One Step PrimeScript III RT-PCR Kit
RR600B One Step PrimeScript™ III RT-PCR Kit 1,000 Rxns USD $1538.00

One Step PrimeScript III RT-qPCR Mix is a dedicated reagent for one-step real-time, probe-based RT-qPCR (using the 5’ nuclease method). This 2X premix does not freeze at its storage temperature of –20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube. The reverse transcription reaction uses the novel PrimeScript III RTase, which displays increased heat tolerance (up to 55℃) while maintaining the specificity and extensibility of PrimeScript RTase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis, TaKaRa Taq HS performs highly specific and efficient PCR amplification, while the fluorescence emitted by the probe is detected in real-time. One Step PrimeScript III RT-qPCR Mix is also highly resistant to a wide variety of inhibitory substances in blood and soil, allowing stable one-step real-time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc.

Cat. # RR600B contains five of Cat. # RR600A. Please refer to Cat. # RR600A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

RR064A One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) 100 Rxns USD $337.00

The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

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RR064A: One Step PrimeScript RT-PCR Kit (Perfect Real Time)

RR064A: One Step PrimeScript RT-PCR Kit (Perfect Real Time)
RR064B One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) 500 Rxns USD $1336.00

The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR. Cat. # RR064B contains 5 of Cat. # RR064A. Please refer to Cat. # RR064A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

More Information

Citations for SARS-CoV-2 detection using Takara Bio RT-PCR kits

  • CDI Enhanced COVID-19 Test EUA Summary, Hackensack University Medical Center (HUMC) Molecular Pathology Laboratory (April 15, 2020). Available at: https://www.fda.gov/media/137036/download
  • COV-19 IDx assay EUA Summary, Ipsum Diagnostics, LLC (April 1, 2020). Available at: https://www.fda.gov/media/136621/download
  • Brown, J. R. et al. Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes. bioRxiv 2020.04.17.047118; doi: https://doi.org/10.1101/2020.04.17.047118
  • Reijns, M. AM. et al. A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection. medRxiv 2020.07.14.20154005; doi: https://doi.org/10.1101/2020.07.14.20154005
  • Zhu, N. et al. A novel coronavirus from patients with pneumonia in China, 2019. N. Engl. J. Med. NEJMoa2001017 (2020).
    • Zhu et al. supplementary appendix: RT-qPCR protocol and primers used 

For product information and a full listing of available sizes, please visit:

  • One Step PrimeScript III RT-PCR Kit product page
  • One Step PrimeScript RT-PCR Kit (Perfect Real Time) product page
  • PrimeDirect Probe RT-qPCR Mix product page

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


SmartChip system dispensing reagents

Save resources and increase sample throughput for COVID-19 detection

The SmartChip Real-Time PCR System is a complete, high-throughput solution with unrivaled flexibility in assay and sample formats. This system supports:

  • 100-nl reaction volumes that decrease costs up to 200X vs. traditional plates
  • 5,184 wells for processing up to 384 samples per chip
  • Automated dispensing that reduces hands-on time
  • 14 different dispense patterns that enable rapid switching, adding, or removing assays and replicates
Learn more

Find more products for COVID-19 research

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  • Direct viral detection via qPCR
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  • Immune profiling
  • Vaccine development
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Helping researchers tackle COVID-19

Know your enemy: finding certainty in uncertain times

This blog highlights one of the first studies identifying and characterizing the novel coronavirus, ultimately contributing to the viral genome sequence being shared worldwide.

Screening for the novel coronavirus with one-step RT-qPCR

Learn how researchers identified a novel, highly contagious coronavirus and developed a one-step RT-qPCR screening protocol.

Takara Bio plays a crucial role in fighting the novel coronavirus

Learn how Takara employees in Dalian, China, are working tirelessly to keep up with the demand for the one-step PrimeScript RT-PCR kit, a crucial component in the detection of the novel coronavirus.

Entering new worlds while staying in place

Hear how a Product Manager in Europe is adjusting to the changing research market in light of COVID-19.

Working hard to meet production demands for SARS-CoV-2 testing

Hear how a Takara Bio employee of 27 years is contributing to the COVID-19 effort.

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Our hiPSC-derived cells and other cell biology research products help you study SARS-CoV-2 infection, identify potential targets for drug discovery, evaluate the metabolism and safety of candidate drugs, and develop therapeutic approaches for COVID-19.

Automated viral detection via high-throughput qPCR

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Takara Bio products in COVID-19 publications

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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