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  • Human BCR profiling kit for Illumina sequencing
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Human BCR profiling kit data View data for this product
Software for BCR/TCR sequencing analysis Cogent NGS Immune Profiler software tool
TCR and BCR profiling tips 4 factors to consider for TCR/BCR profiling
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Human BCR profiling kit data View data for this product
Software for BCR/TCR sequencing analysis Cogent NGS Immune Profiler software tool
TCR and BCR profiling tips 4 factors to consider for TCR/BCR profiling

SMARTer Human BCR IgG IgM H/K/L Profiling Kit—capturing complete V(D)J variable regions of BCR transcripts

SMARTer Human BCR IgG H/K/L Profiling Kit

The new SMARTer Human BCR IgG IgM H/K/L Profiling Kit pairs 5' RACE with NGS technology to provide a sensitive, accurate, and optimized approach to BCR profiling from RNA input samples. The 5' RACE method reduces variability and allows for priming from the constant region of BCR heavy or light chains. This kit combines these benefits with gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts and provide a highly sensitive and reproducible method for profiling B-cell repertoires.

The new SMARTer Human BCR IgG IgM H/K/L Profiling Kit pairs 5' RACE with NGS technology to provide a sensitive, accurate, and optimized approach to BCR profiling from RNA input samples. The 5' RACE method reduces variability and allows for priming from the constant region of BCR heavy or light chains. This kit combines these benefits with gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts and provide a highly sensitive and reproducible method for profiling B-cell repertoires.

The kit is designed to work with a range of RNA input amounts depending on the sample type, and has been shown to generate high-quality, Illumina-ready libraries from as little as 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) and 1 to 100 ng of total RNA from B cells. Data can be generated for both heavy and light chains (kappa and lambda) of human immunoglobulin IgG and IgM. This latest profiling kit also includes unique molecular identifiers (UMI), making it possible to remove reads derived from PCR errors or duplication and/or sequencing errors, thus ensuring more accurate and reliable results

Upon completion of a sequencing run, data can be analyzed with our Cogent NGS Immune Profiler Software, which uses UMI-based error correction. This software enables high-quality BCR profiling analysis, including clonotype count and identification and sequence information for V(D)J regions.

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Cat. # Product Size Price License Quantity Details
634467 SMARTer® Human BCR IgG IgM H/K/L Profiling Kit 48 Rxns USD $3456.00

License Statement

ID Number  
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit enables users to analyze B-cell receptor (BCR) repertoires from total RNA samples. This kit can generate libraries from 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) and 1 to 100 ng of total RNA from B cells. This kit may be used for other sample types, but the protocol may need to be modified. The kit can be used to generate data for both heavy and light chains (kappa and lambda) of human immunoglobulin IgG and IgM.

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of BCR transcripts. It also incorporates unique molecular identifiers (UMI) to facilitate PCR error correction and clonotype quantification during data analysis.

Included in the kit are primers that incorporate Illumina-specific adapter sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms. This kit supports up to 48 rxns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634467: SMARTer Human BCR IgG IgM H/K/L Profiling Kit

634467: SMARTer Human BCR IgG IgM H/K/L Profiling Kit
634466 SMARTer® Human BCR IgG IgM H/K/L Profiling Kit 12 Rxns USD $1190.00

License Statement

ID Number  
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit enables users to analyze B-cell receptor (BCR) repertoires from total RNA samples. This kit can generate libraries from 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) and 1 to 100 ng of total RNA from B cells. This kit may be used for other sample types, but the protocol may need to be modified. The kit can be used to generate data for both heavy and light chains (kappa and lambda) of human immunoglobulin IgG and IgM.

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit leverages SMART technology and employs a 5' RACE-like approach to capture complete V(D)J variable regions of BCR transcripts. It also incorporates unique molecular identifiers (UMI) to facilitate PCR error correction and clonotype quantification during data analysis.

Included in the kit are primers that incorporate Illumina-specific adapter sequences during cDNA amplification. The protocol generates indexed libraries that are ready for sequencing on Illumina platforms. This kit supports up to 12 rxns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

634466: SMARTer Human BCR IgG IgM H/K/L Profiling Kit

634466: SMARTer Human BCR IgG IgM H/K/L Profiling Kit

Overview

  • Input: 10 ng to 1 µg of total RNA from peripheral blood mononuclear cells (PBMCs) or 1 to 100 ng of total RNA from B cells
  • UMI-based correction for removal of reads derived from PCR error or duplication and/or sequencing errors
  • Sensitive and specific clonotype detection with optimized cDNA library generation
  • Accurate amplification of human IgG subclasses and identification via sequencing in a majority of cases
  • Optimized PCR pooling strategy for highly sensitive detection of different chains from the same sample
  • Flexible pooling strategies accommodate different experimental requirements regarding alignment and identification of primary chain sequences
  • Complete workflow with the use of our Cogent NGS Immune Profiler Software for sequencing data analysis
     - The Cogent NGS Immune Profiler Software is free to use

More Information

Applications

  • Human BCR repertoire analysis (heavy and light [kappa and lambda] chains)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Human BCR profiling data

Profiling human B-cell receptors with a 5' RACE approach and SMART technology

The SMARTer Human BCR IgG IgM H/K/L Profiling Kit captures entire V(D)J variable regions of BCR transcripts, allowing you to reach a complete and accurate view of human BCR repertoires. Incorporation of UMIs further improves accuracy and reliability by enabling the removal of reads derived from PCR errors or duplication and/or sequencing errors.

View the data

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