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  • SMART-Seq Mouse BCR (with UMIs)
  • SMART-Seq Mouse TCR (with UMIs)
  • Mouse BCR profiling kit for Illumina sequencing
  • Mouse TCR profiling kit for Illumina sequencing
thumbnail of data generated with the SMART-Seq Mouse BCR (with UMIs) kit Track B-cell changes in your mouse model
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Immune profiling

  • Human repertoire
    • SMART-Seq Human BCR (with UMIs)
    • Human BCR profiling kit for Illumina sequencing
    • SMART-Seq Human TCR (with UMIs)
    • Human TCRv2 profiling kit for Illumina sequencing
    • Human scTCR profiling kit for Illumina sequencing
  • Mouse repertoire
    • SMART-Seq Mouse BCR (with UMIs)
    • SMART-Seq Mouse TCR (with UMIs)
    • Mouse BCR profiling kit for Illumina sequencing
    • Mouse TCR profiling kit for Illumina sequencing
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thumbnail of data generated with the SMART-Seq Mouse BCR (with UMIs) kit Track B-cell changes in your mouse model

SMART-Seq Mouse BCR (with UMIs)

SMART-Seq Mouse BCR (with UMIs) enables detection of mouse BCR sequences from all BCR isotypes (IgA/D/E/G/M on heavy chain; IgK/L on light chain). Powered by SMART (Switching Mechanism at 5' end of RNA Template) technology, the kit combines NGS with a 5’-RACE like approach to capture the full-length V(D)J sequences of mouse B-cell receptors.

SMART-Seq Mouse BCR (with UMIs) enables detection of mouse BCR sequences from all BCR isotypes (IgA/D/E/G/M on heavy chain; IgK/L on light chain). Powered by SMART (Switching Mechanism at 5' end of RNA Template) technology, the kit combines NGS with a 5’-RACE like approach to capture the full-length V(D)J sequences of mouse B-cell receptors.

The kit is designed to work with high-quality mouse RNA (RIN>7) spanning a wide range: 10 ng–1 μg of total RNA from mouse spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs). It also works with other sample types such as RNA extracted from mouse blood or lymph nodes.

BCR library preparation can now be streamlined with provided primer pools and enzyme premix, reducing the number of tubes, reagents, and steps. Unique molecular identifiers (UMIs) are also incorporated into the workflow to remove PCR bias and sequencing errors during data analysis, thus guaranteeing accuracy. Moreover, this kit is compatible with the Unique Dual Index Kits (Cat. # 634752–56; sold separately) so up to 384 BCR libraries can be pooled together in a single flow cell to save sequencing cost. Protocol is also automation friendly, so you can easily miniaturize to reduce the cost-per-reaction and increase throughput.

With this kit, you can also enjoy sequencing flexibility and the library products can be sequenced on any Illumina sequencer. The full-length V(D)J area can be sequenced if more data is desired, or you can focus on just the CDR3 region to save on sequencing costs.

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Cat. # Product Size Price License Quantity Details
634352 SMART-Seq® Mouse BCR (with UMIs) 24 Rxns USD $1406.00

License Statement

ID Number  
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 
455 This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

SMART-Seq Mouse BCR (with UMIs) enables users to analyze B-cell receptor (BCR) repertoires from 10 ng–1 µg high-integrity (RIN>7) bulk total RNA samples from spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs) or RNA extracted from mouse whole blood (RNA input ≥100 ng) or lymph nodes. The kit can be used to generate data for both heavy (IgG, IgM, IgA, IgD, and IgE) and light chains (IgK, IgL) of mouse B-cell receptors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634352: SMART-Seq Mouse BCR (with UMIs)

634352:  SMART-Seq Mouse BCR (with UMIs)

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Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

Schematic of technology and workflow for SMART-Seq Muman BCR (with UMIs). Input RNA or cells are oligo-dT primed using the dT Primer (dark blue). Following oligo dT-priming, SmartScribe Reverse Transcriptase performs first strand cDNA synthesis on input RNA or cells and adds non-templated nucleotides to the 5′ end of each cDNA molecule (XXXXX). Upon reaching the 5′ end of the RNA template, the SMART UMI Oligo anneals to the non-templated nucleotides (XXXXX), incorporating the UMI (yellow) and partial Illumina adapter (light green) complementary to the mBCR PCR1 Universal Forward primer. Following reverse transcription, semi-nested PCR is performed to amplify BCR cDNAs. In PCR1, the mBCR PCR1 Universal Forward primer anneals to the complementary sequence carried by the SMART UMI Oligo (light green), incorporating Illumina Read2 sequence (dark green). The mBCR PCR1 Reverse primer (orange) anneals to sequences in the constant regions of BCR cDNAs to amplify the entire BCR V(D)J region. During the PCR2, the nested mBCR LC (light chain) or mBCR HC (heavy chain) PCR2 Reverse primers anneal to sequences in BCR constant regions that are internal to the sequences bound by the mBCR PCR1 Reverse primer and add the Illumina Read 1 sequence (light purple). In the same PCR2 reaction, Unique Dual Index Kit primers anneal to sequence added by mBCR PCR1 Universal Forward primer or the mBCR LC or HC PCR2 Reverse primers to add Illumina P7-i7 and P5-i5 index sequences (dark blue). The result is a sequencing-ready library that contains the entire BCR light chain and/or heavy chain variable regions with a small portion of the constant region.

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mBCRv2 detected all BCR isotypes with outstanding sensitivity

 mBCRv2 detected all BCR isotypes with outstanding sensitivity

Panel A. Detection of all BCR isotypes for both heavy chain (IgA/D/G/M, except IgE due to its very low expression in normal mouse samples) and light chain (IgK/L) by SMART-Seq Mouse BCR (with UMIs) in 10, 100, and 1,000 ng input of mouse spleen RNA. Comparison across various RNA inputs shows a consistent increase in clonotype counts with higher input. Panel B. Sensitivity of SMART-Seq Mouse BCR (with UMIs) ensures detection of more heavy-chain and light-chain clonotypes with more input RNA. Bar graphs illustrate both heavy-chain and light-chain clonotype counts consistently increase with a higher amount of input RNA.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

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634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
744970.5 NucleoMag® NGS Clean-up and Size Select 5 mL USD $113.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension.

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744970.5: NucleoMag NGS Clean-up and Size Select

744970.5: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

Back

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

Back

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

Back

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Back

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
744970.50 NucleoMag® NGS Clean-up and Size Select 50 mL USD $770.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension.

Documents Image Data

Back

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

Back

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

Back

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

Back

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Back

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Back

744970.50: NucleoMag NGS Clean-up and Size Select

744970.50: NucleoMag NGS Clean-up and Size Select
744970.500 NucleoMag® NGS Clean-up and Size Select 500 mL USD $5305.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension.

Documents Image Data

Back

744970.500: NucleoMag NGS Clean-up and Size Select

744970.500: NucleoMag NGS Clean-up and Size Select

Back

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

Back

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

Back

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

Back

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Back

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
634353 SMART-Seq® Mouse BCR (with UMIs) 96 Rxns USD $4983.00

License Statement

ID Number  
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 
455 This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

SMART-Seq Mouse BCR (with UMIs) enables users to analyze B-cell receptor (BCR) repertoires from 10 ng–1 µg high-integrity (RIN>7) bulk total RNA samples from spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs) or RNA extracted from mouse whole blood (RNA input ≥100 ng) or lymph nodes. The kit can be used to generate data for both heavy (IgG, IgM, IgA, IgD, and IgE) and light chains (IgK, IgL) of mouse B-cell receptors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

Schematic of technology and workflow for SMART-Seq Muman BCR (with UMIs). Input RNA or cells are oligo-dT primed using the dT Primer (dark blue). Following oligo dT-priming, SmartScribe Reverse Transcriptase performs first strand cDNA synthesis on input RNA or cells and adds non-templated nucleotides to the 5′ end of each cDNA molecule (XXXXX). Upon reaching the 5′ end of the RNA template, the SMART UMI Oligo anneals to the non-templated nucleotides (XXXXX), incorporating the UMI (yellow) and partial Illumina adapter (light green) complementary to the mBCR PCR1 Universal Forward primer. Following reverse transcription, semi-nested PCR is performed to amplify BCR cDNAs. In PCR1, the mBCR PCR1 Universal Forward primer anneals to the complementary sequence carried by the SMART UMI Oligo (light green), incorporating Illumina Read2 sequence (dark green). The mBCR PCR1 Reverse primer (orange) anneals to sequences in the constant regions of BCR cDNAs to amplify the entire BCR V(D)J region. During the PCR2, the nested mBCR LC (light chain) or mBCR HC (heavy chain) PCR2 Reverse primers anneal to sequences in BCR constant regions that are internal to the sequences bound by the mBCR PCR1 Reverse primer and add the Illumina Read 1 sequence (light purple). In the same PCR2 reaction, Unique Dual Index Kit primers anneal to sequence added by mBCR PCR1 Universal Forward primer or the mBCR LC or HC PCR2 Reverse primers to add Illumina P7-i7 and P5-i5 index sequences (dark blue). The result is a sequencing-ready library that contains the entire BCR light chain and/or heavy chain variable regions with a small portion of the constant region.

Back

mBCRv2 detected all BCR isotypes with outstanding sensitivity

 mBCRv2 detected all BCR isotypes with outstanding sensitivity

Panel A. Detection of all BCR isotypes for both heavy chain (IgA/D/G/M, except IgE due to its very low expression in normal mouse samples) and light chain (IgK/L) by SMART-Seq Mouse BCR (with UMIs) in 10, 100, and 1,000 ng input of mouse spleen RNA. Comparison across various RNA inputs shows a consistent increase in clonotype counts with higher input. Panel B. Sensitivity of SMART-Seq Mouse BCR (with UMIs) ensures detection of more heavy-chain and light-chain clonotypes with more input RNA. Bar graphs illustrate both heavy-chain and light-chain clonotype counts consistently increase with a higher amount of input RNA.

Back

634353: SMART-Seq Mouse BCR (with UMIs)

634353:  SMART-Seq Mouse BCR (with UMIs)

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

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634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
744970.5 NucleoMag® NGS Clean-up and Size Select 5 mL USD $113.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension.

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744970.5: NucleoMag NGS Clean-up and Size Select

744970.5: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
744970.50 NucleoMag® NGS Clean-up and Size Select 50 mL USD $770.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension.

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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744970.50: NucleoMag NGS Clean-up and Size Select

744970.50: NucleoMag NGS Clean-up and Size Select
744970.500 NucleoMag® NGS Clean-up and Size Select 500 mL USD $5305.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension.

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744970.500: NucleoMag NGS Clean-up and Size Select

744970.500: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

Back

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

Back

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
634351 SMART-Seq® Mouse BCR (with UMIs) 4 x 96 Rxns USD $18159.00

License Statement

ID Number  
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 

SMART-Seq Mouse BCR (with UMIs) enables users to analyze B-cell receptor (BCR) repertoires from 10 ng–1 µg high-integrity (RIN>7) bulk total RNA samples from spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs) or RNA extracted from mouse whole blood (RNA input ≥100 ng) or lymph nodes. The kit can be used to generate data for both heavy (IgG, IgM, IgA, IgD, and IgE) and light chains (IgK, IgL) of mouse B-cell receptors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634351: SMART-Seq Mouse BCR (with UMIs)

634351:  SMART-Seq Mouse BCR (with UMIs)

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Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

Schematic of technology and workflow for SMART-Seq Muman BCR (with UMIs). Input RNA or cells are oligo-dT primed using the dT Primer (dark blue). Following oligo dT-priming, SmartScribe Reverse Transcriptase performs first strand cDNA synthesis on input RNA or cells and adds non-templated nucleotides to the 5′ end of each cDNA molecule (XXXXX). Upon reaching the 5′ end of the RNA template, the SMART UMI Oligo anneals to the non-templated nucleotides (XXXXX), incorporating the UMI (yellow) and partial Illumina adapter (light green) complementary to the mBCR PCR1 Universal Forward primer. Following reverse transcription, semi-nested PCR is performed to amplify BCR cDNAs. In PCR1, the mBCR PCR1 Universal Forward primer anneals to the complementary sequence carried by the SMART UMI Oligo (light green), incorporating Illumina Read2 sequence (dark green). The mBCR PCR1 Reverse primer (orange) anneals to sequences in the constant regions of BCR cDNAs to amplify the entire BCR V(D)J region. During the PCR2, the nested mBCR LC (light chain) or mBCR HC (heavy chain) PCR2 Reverse primers anneal to sequences in BCR constant regions that are internal to the sequences bound by the mBCR PCR1 Reverse primer and add the Illumina Read 1 sequence (light purple). In the same PCR2 reaction, Unique Dual Index Kit primers anneal to sequence added by mBCR PCR1 Universal Forward primer or the mBCR LC or HC PCR2 Reverse primers to add Illumina P7-i7 and P5-i5 index sequences (dark blue). The result is a sequencing-ready library that contains the entire BCR light chain and/or heavy chain variable regions with a small portion of the constant region.

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mBCRv2 detected all BCR isotypes with outstanding sensitivity

 mBCRv2 detected all BCR isotypes with outstanding sensitivity

Panel A. Detection of all BCR isotypes for both heavy chain (IgA/D/G/M, except IgE due to its very low expression in normal mouse samples) and light chain (IgK/L) by SMART-Seq Mouse BCR (with UMIs) in 10, 100, and 1,000 ng input of mouse spleen RNA. Comparison across various RNA inputs shows a consistent increase in clonotype counts with higher input. Panel B. Sensitivity of SMART-Seq Mouse BCR (with UMIs) ensures detection of more heavy-chain and light-chain clonotypes with more input RNA. Bar graphs illustrate both heavy-chain and light-chain clonotype counts consistently increase with a higher amount of input RNA.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
744970.5 NucleoMag® NGS Clean-up and Size Select 5 mL USD $113.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension.

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744970.5: NucleoMag NGS Clean-up and Size Select

744970.5: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

Back

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
744970.50 NucleoMag® NGS Clean-up and Size Select 50 mL USD $770.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension.

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

Back

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

Back

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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744970.50: NucleoMag NGS Clean-up and Size Select

744970.50: NucleoMag NGS Clean-up and Size Select
744970.500 NucleoMag® NGS Clean-up and Size Select 500 mL USD $5305.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension.

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744970.500: NucleoMag NGS Clean-up and Size Select

744970.500: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Back

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Unique Dual Index Kits
Cogent NGS Immune Viewer

Overview

  • Complete BCR repertoire profiling: includes all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains
  • Supports various inputs: 10 ng–1 μg of high-integrity RNA (RIN>7) from mouse spleen, bone marrow, lymph nodes, whole blood, and PBMCs
  • Streamlined workflow: primer pools for heavy and light immunoglobulin chains and enzyme premixes provided
  • Increased accuracy: UMI-based analysis controls for PCR duplicates and errors, correcting for artifacts in clonotype diversity and abundance assessments
  • Flexible: sequence either the full-length V(D)J region or the CDR3 only and multiplex with up to 384 UDIs
  • Economical and high-throughput friendly: can be miniaturized to reduce costs or automated

Achieve sensitive and reproducible BCR clonotype detection

SMART-Seq Mouse BCR (with UMIs) detected all BCR isotypes with outstanding sensitivity. Mouse BCR heavy chain (IgA/D/G/E/M) and light chain (IgK/L) libraries were generated from 10, 100, and 1,000 ng of mouse spleen RNA using SMART-Seq Mouse BCR (with UMIs). The sequencing reads were processed using Cogent NGS Immune Profiler Software.


More Information

Applications

  • Analyze B-cell clonal organization and diversity in tissues and circulation to understand how they affect B-cell differentiation pathway and functionalities.
  • Find BCRs with high affinity to inform better vaccination or therapy against infectious diseases.
  • Track changes and migration of antigen-specific B cells post adoptive transfer in mouse disease models for HIV and other diseases.
  • Check BCR sequences to study development of autoantibodies against new epitopes over time and how that correlates with severity of autoimmune diseases like lupus.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Clontech, TaKaRa, cellartis

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  • Most popular polymerases
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