Vaccine-grade plasmid DNA purification—NucleoBond RS

NucleoBond RS columns enable large-scale isolation of vaccine-grade, endotoxin-free plasmid DNA from bacterial cultures utilizing peristaltic pumps or FPLC systems. The NucleoBond RS workflow achieves endotoxin levels ≤0.01 EU/μg DNA, ensuring compliance for processes with high regulatory standards such as mRNA-based vaccine production. After two efficient washing steps that remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. In addition, RS columns are suitable for preparative scale RNA Clean-up from pre-purified RNA or IVT reactions.

NucleoBond RS columns enable large-scale isolation of vaccine-grade, endotoxin-free plasmid DNA from bacterial cultures utilizing peristaltic pumps or FPLC systems. The NucleoBond RS workflow achieves endotoxin levels ≤ 0.01 EU/μg DNA, ensuring compliance for processes with high regulatory standards such as mRNA-based vaccine production. After two efficient washing steps that remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H₂O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. In addition, RS columns are suitable for preparative scale RNA clean-up from pre-purified RNA or IVT reactions.

For maximum flexibility, all components needed for plasmid purification or RNA clean-up using NucleoBond RS columns are offered as standalone products. For more detailed information regarding equipment and quantities of individual components, please refer to recommended reagents and equipment below.

More Less

Loading product information...

Overview

A modular system for vaccine-grade plasmid purification or post-IVT RNA clean-up

Achieve endotoxin levels ≤0.01 EU/μg DNA
Process plasmids or post-IVT RNA using closed columns compatible with peristaltic pumps or FPLC
Scale up from 10 mg to 200 mg bacterial culture (or 1 mg to 140 mg post-IVT RNA) with four column sizes

Recommended reagents for plasmid purification

Product	Size	Specification	Cat. #
Buffer RES-EF	1,000 mL	Resuspension buffer	740386.1000
Buffer LYS-EF	1,000 mL	Lysis buffer	740387.1000
Buffer NEU-EF	1,000 mL	Neutralization buffer	740388.1000
Buffer EQ-EF	1,000 mL 3,000 mL	Equilibration buffer	740380.1000 740380.3000
Buffer BMC-EF*	1,000 mL 3,000 mL	Binding mediating reconstitution buffer	740382.1000 740382.3000
Buffer ENTO-EF	1,000 mL 3,000 mL	Endotoxin removal buffer	740381.1000 740381.3000
Buffer WASH-EF	1,000 mL	Wash buffer	740392.1000
Buffer ELU-EF	900 mL	Elution buffer	740393.900

*Buffer BMC-EF is needed for the alternative RNAse-free NucleoBond RS plasmid purification workflow. Please refer to the NucleoBond RS Endotoxin-Free Plasmid DNA Purification User Manual for more information.

Product	Size	Specification	Cat. #
Buffer NRB	1,000 mL 3,000 mL	Binding buffer	740371.1000 740371.3000
Buffer EQU	1,000 mL	Equilibration buffer	740317.1000
Buffer NRW	1,000 mL 3,000 mL	Wash buffer	740372.1000 740372.3000
Buffer NRE	1,000 mL 3,000 mL	Elution buffer	740373.1000 740373.3000

Additional recommended reagents and equipment

Product	Size	Specification	Cat. #
Buffer TE-EF	1,000 mL	Endotoxin-free TE buffer	740797.1
Buffer H₂O-EF	1,000 mL	Endotoxin-free water	740798.1
RNase A	50 mg 100 mg	Enzymatic degradation and removal of RNA	740505.50 740505.100
NucleoBond Bottle Top Filter Type 2	5 Filters	Lysate filtration	740553.5
NucleoVac 96 Vacuum Regulator	1 piece	Vacuum manifold for controlling vacuum	740641

More Information

Specifications of plasmid DNA isolated using NucleoBond RS columns

Parameter	Method	Criterion
Integrity	Agarose gel electrophoresis/fragment analyzer	≥90% supercoiled form
Purity	UV spectrum	A₂₆₀/A₂₈₀ 1.8–2.0 A₂₆₀/A₂₃₀ 1.9–2.3
Endotoxin levels	LAL/rFC	≤0.01 EU/μg DNA
Host DNA	gDNA PCR	≤1%
Host RNA	Agarose gel electrophoresis Fluorometric quantification	Not visible at 200 ng DNA ≤2%
Host proteins	BCA	≤1%

Applications

Large-scale, endotoxin-free plasmid purification
Preparation of vaccine-grade plasmid DNA
Post-IVT RNA clean-up

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2026 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.